Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5650 and Pf1N1B4_5651 are separated by 184 nucleotides Pf1N1B4_5651 and Pf1N1B4_5652 are separated by 119 nucleotides Pf1N1B4_5652 and Pf1N1B4_5653 are separated by 149 nucleotides
Pf1N1B4_5650 - Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes, at 77,942 to 79,588
_5650
Pf1N1B4_5651 - ChlD component of cobalt chelatase involved in B12 biosynthesis, at 79,773 to 80,318
_5651
Pf1N1B4_5652 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 80,438 to 81,448
_5652
Pf1N1B4_5653 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 81,598 to 85,464
_5653
Position (kb) 80
81
82 Strain fitness (log2 ratio) -2
-1
0
1
2 at 79.539 kb on + strand at 79.540 kb on - strand at 79.797 kb on - strand at 80.247 kb on + strand, within Pf1N1B4_5651 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.171 kb on - strand, within Pf1N1B4_5652 at 81.614 kb on - strand at 81.614 kb on - strand at 81.768 kb on - strand at 81.768 kb on - strand at 81.870 kb on + strand at 81.870 kb on + strand at 81.871 kb on - strand at 81.871 kb on - strand at 82.058 kb on + strand, within Pf1N1B4_5653
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 79,539 + -2.4 79,540 - -2.2 79,797 - -0.4 80,247 + Pf1N1B4_5651 0.87 +1.9 81,141 + Pf1N1B4_5652 0.70 +1.6 81,141 + Pf1N1B4_5652 0.70 -1.4 81,142 - Pf1N1B4_5652 0.70 +0.2 81,142 - Pf1N1B4_5652 0.70 -0.7 81,142 - Pf1N1B4_5652 0.70 -0.8 81,142 - Pf1N1B4_5652 0.70 -2.0 81,142 - Pf1N1B4_5652 0.70 -1.0 81,171 - Pf1N1B4_5652 0.73 +0.2 81,614 - -0.8 81,614 - -1.4 81,768 - +0.0 81,768 - -0.4 81,870 + -2.4 81,870 + -0.8 81,871 - +0.1 81,871 - +0.1 82,058 + Pf1N1B4_5653 0.12 -1.6
Or see this region's nucleotide sequence