Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5255 and Pf1N1B4_5256 are separated by 149 nucleotides Pf1N1B4_5256 and Pf1N1B4_5257 are separated by 546 nucleotides Pf1N1B4_5257 and Pf1N1B4_5258 are separated by 78 nucleotides Pf1N1B4_5258 and Pf1N1B4_5259 are separated by 314 nucleotides
Pf1N1B4_5255 - hypothetical protein, at 14,928 to 15,152
_5255
Pf1N1B4_5256 - hypothetical protein, at 15,302 to 15,460
_5256
Pf1N1B4_5257 - hypothetical protein, at 16,007 to 16,306
_5257
Pf1N1B4_5258 - hypothetical protein, at 16,385 to 16,576
_5258
Pf1N1B4_5259 - hypothetical protein, at 16,891 to 17,250
_5259
Position (kb)
16
17 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 15.028 kb on + strand, within Pf1N1B4_5255 at 15.073 kb on + strand, within Pf1N1B4_5255 at 15.074 kb on - strand, within Pf1N1B4_5255 at 15.125 kb on + strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.249 kb on - strand at 15.298 kb on - strand at 15.303 kb on + strand at 15.332 kb on + strand, within Pf1N1B4_5256 at 15.333 kb on - strand, within Pf1N1B4_5256 at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.499 kb on - strand at 15.499 kb on - strand at 15.581 kb on - strand at 15.604 kb on + strand at 15.757 kb on + strand at 15.758 kb on - strand at 15.758 kb on - strand at 15.794 kb on + strand at 15.794 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.037 kb on + strand at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.213 kb on + strand, within Pf1N1B4_5257 at 16.213 kb on + strand, within Pf1N1B4_5257 at 16.213 kb on + strand, within Pf1N1B4_5257 at 16.213 kb on + strand, within Pf1N1B4_5257 at 16.214 kb on - strand at 16.214 kb on - strand, within Pf1N1B4_5257 at 16.268 kb on + strand, within Pf1N1B4_5257 at 16.268 kb on + strand, within Pf1N1B4_5257 at 16.280 kb on + strand at 16.280 kb on + strand at 16.280 kb on + strand at 16.384 kb on + strand at 16.384 kb on + strand at 16.384 kb on + strand at 16.409 kb on + strand, within Pf1N1B4_5258 at 16.434 kb on + strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.509 kb on + strand, within Pf1N1B4_5258 at 16.510 kb on - strand, within Pf1N1B4_5258 at 16.510 kb on - strand, within Pf1N1B4_5258 at 16.563 kb on - strand at 16.575 kb on - strand at 16.623 kb on + strand at 16.623 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.628 kb on - strand at 16.628 kb on - strand at 16.648 kb on + strand at 16.648 kb on + strand at 16.676 kb on + strand at 16.676 kb on + strand at 16.676 kb on + strand at 16.747 kb on + strand at 16.761 kb on + strand at 16.762 kb on - strand at 16.809 kb on - strand at 16.918 kb on + strand at 16.918 kb on + strand at 16.918 kb on + strand at 16.918 kb on + strand at 16.918 kb on + strand at 16.919 kb on - strand at 16.940 kb on + strand, within Pf1N1B4_5259 at 16.940 kb on + strand, within Pf1N1B4_5259 at 16.941 kb on - strand, within Pf1N1B4_5259 at 16.941 kb on - strand, within Pf1N1B4_5259 at 16.941 kb on - strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.151 kb on - strand, within Pf1N1B4_5259 at 17.151 kb on - strand, within Pf1N1B4_5259 at 17.151 kb on - strand, within Pf1N1B4_5259 at 17.151 kb on - strand, within Pf1N1B4_5259 at 17.163 kb on + strand, within Pf1N1B4_5259 at 17.164 kb on - strand, within Pf1N1B4_5259 at 17.244 kb on + strand at 17.244 kb on + strand at 17.245 kb on - strand at 17.268 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 15,028 + Pf1N1B4_5255 0.44 +0.7 15,073 + Pf1N1B4_5255 0.64 +0.6 15,074 - Pf1N1B4_5255 0.65 -1.2 15,125 + Pf1N1B4_5255 0.88 -0.8 15,126 - Pf1N1B4_5255 0.88 +0.0 15,126 - Pf1N1B4_5255 0.88 +0.0 15,126 - Pf1N1B4_5255 0.88 -1.0 15,249 - -0.5 15,298 - -1.0 15,303 + +1.0 15,332 + Pf1N1B4_5256 0.19 -0.4 15,333 - Pf1N1B4_5256 0.19 -1.0 15,498 + -0.5 15,498 + +1.2 15,498 + +0.6 15,498 + +0.4 15,498 + -0.1 15,498 + +0.8 15,499 - +1.4 15,499 - -0.5 15,581 - -1.2 15,604 + -1.5 15,757 + +0.0 15,758 - -0.6 15,758 - +1.4 15,794 + -0.0 15,794 + +0.5 15,889 + +0.9 15,889 + -0.5 15,889 + +1.2 15,889 + -0.7 15,889 + -0.3 15,889 + +0.4 15,889 + +0.8 15,889 + -2.0 15,889 + +0.0 15,889 + -0.2 15,890 - -0.2 15,890 - +0.6 15,890 - -0.4 15,890 - +0.0 15,890 - -0.7 15,890 - +0.4 15,890 - +0.4 15,890 - +0.0 15,890 - +0.0 16,037 + Pf1N1B4_5257 0.10 -0.3 16,037 + Pf1N1B4_5257 0.10 -2.3 16,037 + +0.0 16,037 + Pf1N1B4_5257 0.10 -0.5 16,038 - Pf1N1B4_5257 0.10 -1.1 16,038 - Pf1N1B4_5257 0.10 -0.7 16,038 - Pf1N1B4_5257 0.10 -0.4 16,038 - Pf1N1B4_5257 0.10 -2.3 16,213 + Pf1N1B4_5257 0.69 +0.3 16,213 + Pf1N1B4_5257 0.69 -1.4 16,213 + Pf1N1B4_5257 0.69 -0.4 16,213 + Pf1N1B4_5257 0.69 -1.0 16,214 - +0.0 16,214 - Pf1N1B4_5257 0.69 +0.0 16,268 + Pf1N1B4_5257 0.87 -2.0 16,268 + Pf1N1B4_5257 0.87 -0.5 16,280 + +0.8 16,280 + +0.6 16,280 + -0.6 16,384 + -1.5 16,384 + -0.4 16,384 + +0.8 16,409 + Pf1N1B4_5258 0.12 +0.8 16,434 + Pf1N1B4_5258 0.26 -1.0 16,435 - Pf1N1B4_5258 0.26 +2.0 16,435 - Pf1N1B4_5258 0.26 -1.0 16,435 - Pf1N1B4_5258 0.26 +0.5 16,435 - Pf1N1B4_5258 0.26 +0.6 16,435 - Pf1N1B4_5258 0.26 +2.4 16,435 - Pf1N1B4_5258 0.26 -1.3 16,509 + Pf1N1B4_5258 0.65 -1.5 16,510 - Pf1N1B4_5258 0.65 -1.0 16,510 - Pf1N1B4_5258 0.65 +0.3 16,563 - +1.8 16,575 - +0.6 16,623 + -1.2 16,623 + +0.2 16,627 + -1.0 16,627 + -1.0 16,627 + +0.6 16,627 + -2.0 16,627 + +0.8 16,627 + +1.6 16,627 + +0.0 16,628 - -0.1 16,628 - +1.0 16,648 + -0.3 16,648 + +0.0 16,676 + +1.0 16,676 + -0.1 16,676 + -1.0 16,747 + -0.1 16,761 + +0.8 16,762 - +1.8 16,809 - -0.3 16,918 + +1.0 16,918 + +2.0 16,918 + -2.0 16,918 + -1.0 16,918 + +1.3 16,919 - -0.5 16,940 + Pf1N1B4_5259 0.14 +0.4 16,940 + Pf1N1B4_5259 0.14 +0.6 16,941 - Pf1N1B4_5259 0.14 +1.0 16,941 - Pf1N1B4_5259 0.14 -1.0 16,941 - Pf1N1B4_5259 0.14 +0.7 17,150 + Pf1N1B4_5259 0.72 -1.2 17,150 + Pf1N1B4_5259 0.72 -1.3 17,150 + Pf1N1B4_5259 0.72 +1.0 17,150 + Pf1N1B4_5259 0.72 -1.7 17,150 + Pf1N1B4_5259 0.72 -2.1 17,150 + Pf1N1B4_5259 0.72 -0.6 17,150 + Pf1N1B4_5259 0.72 +0.0 17,151 - Pf1N1B4_5259 0.72 -1.3 17,151 - Pf1N1B4_5259 0.72 -0.4 17,151 - Pf1N1B4_5259 0.72 +0.0 17,151 - Pf1N1B4_5259 0.72 -0.4 17,163 + Pf1N1B4_5259 0.76 +0.6 17,164 - Pf1N1B4_5259 0.76 +0.6 17,244 + -1.5 17,244 + -0.5 17,245 - +0.4 17,268 + +0.0
Or see this region's nucleotide sequence