Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5045 and Pf1N1B4_5046 overlap by 1 nucleotides Pf1N1B4_5046 and Pf1N1B4_5047 are separated by 184 nucleotides Pf1N1B4_5047 and Pf1N1B4_5048 are separated by 246 nucleotides
Pf1N1B4_5045 - Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone, at 5,590,273 to 5,590,878
_5045
Pf1N1B4_5046 - Copper metallochaperone, bacterial analog of Cox17 protein, at 5,590,878 to 5,591,351
_5046
Pf1N1B4_5047 - Integral membrane protein TerC, at 5,591,536 to 5,592,507
_5047
Pf1N1B4_5048 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 5,592,754 to 5,593,680
_5048
Position (kb) 5591
5592
5593 Strain fitness (log2 ratio) -2
-1
0
1 at 5590.672 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.890 kb on + strand at 5590.890 kb on + strand at 5590.890 kb on + strand at 5590.891 kb on - strand at 5590.891 kb on - strand at 5590.891 kb on - strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.902 kb on - strand at 5590.904 kb on + strand at 5590.905 kb on - strand at 5590.905 kb on - strand at 5591.974 kb on + strand at 5591.975 kb on - strand, within Pf1N1B4_5047 at 5592.048 kb on + strand, within Pf1N1B4_5047 at 5592.059 kb on + strand, within Pf1N1B4_5047 at 5592.071 kb on + strand, within Pf1N1B4_5047 at 5592.295 kb on + strand, within Pf1N1B4_5047 at 5592.356 kb on + strand, within Pf1N1B4_5047 at 5592.612 kb on - strand at 5592.661 kb on - strand at 5592.661 kb on - strand at 5592.865 kb on + strand, within Pf1N1B4_5048 at 5592.917 kb on - strand at 5592.917 kb on - strand, within Pf1N1B4_5048 at 5592.917 kb on - strand, within Pf1N1B4_5048
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 5,590,672 + Pf1N1B4_5045 0.66 +0.5 5,590,750 + Pf1N1B4_5045 0.79 +0.0 5,590,750 + Pf1N1B4_5045 0.79 -1.6 5,590,750 + Pf1N1B4_5045 0.79 -1.6 5,590,750 + Pf1N1B4_5045 0.79 -2.1 5,590,750 + +0.8 5,590,751 - Pf1N1B4_5045 0.79 +0.0 5,590,751 - Pf1N1B4_5045 0.79 -1.0 5,590,751 - Pf1N1B4_5045 0.79 +0.8 5,590,890 + -0.6 5,590,890 + -1.0 5,590,890 + +0.6 5,590,891 - +0.4 5,590,891 - -1.6 5,590,891 - -2.1 5,590,901 + +1.0 5,590,901 + +0.7 5,590,901 + -1.9 5,590,901 + -0.1 5,590,901 + +1.4 5,590,901 + -0.2 5,590,902 - -0.6 5,590,904 + +0.4 5,590,905 - +1.4 5,590,905 - -1.6 5,591,974 + -0.6 5,591,975 - Pf1N1B4_5047 0.45 -1.9 5,592,048 + Pf1N1B4_5047 0.53 -0.1 5,592,059 + Pf1N1B4_5047 0.54 -0.8 5,592,071 + Pf1N1B4_5047 0.55 -1.6 5,592,295 + Pf1N1B4_5047 0.78 +0.0 5,592,356 + Pf1N1B4_5047 0.84 -1.6 5,592,612 - +0.4 5,592,661 - +0.0 5,592,661 - +0.0 5,592,865 + Pf1N1B4_5048 0.12 -1.6 5,592,917 - -0.6 5,592,917 - Pf1N1B4_5048 0.18 +0.2 5,592,917 - Pf1N1B4_5048 0.18 +0.0
Or see this region's nucleotide sequence