Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4847 and Pf1N1B4_4848 are separated by 26 nucleotides Pf1N1B4_4848 and Pf1N1B4_4849 are separated by 10 nucleotides Pf1N1B4_4849 and Pf1N1B4_4850 are separated by 163 nucleotides
Pf1N1B4_4847 - Various polyols ABC transporter, ATP-binding component, at 5,355,866 to 5,356,969
_4847
Pf1N1B4_4848 - Various polyols ABC transporter, permease component 2, at 5,356,996 to 5,357,826
_4848
Pf1N1B4_4849 - Various polyols ABC transporter, permease component 1, at 5,357,837 to 5,358,736
_4849
Pf1N1B4_4850 - Various polyols ABC transporter, periplasmic substrate-binding protein, at 5,358,900 to 5,360,222
_4850
Position (kb)
5357
5358
5359 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5356.994 kb on - strand at 5357.342 kb on + strand, within Pf1N1B4_4848 at 5357.343 kb on - strand, within Pf1N1B4_4848 at 5357.343 kb on - strand at 5357.348 kb on + strand, within Pf1N1B4_4848 at 5357.349 kb on - strand, within Pf1N1B4_4848 at 5357.387 kb on + strand, within Pf1N1B4_4848 at 5357.445 kb on - strand, within Pf1N1B4_4848 at 5357.528 kb on + strand, within Pf1N1B4_4848 at 5357.529 kb on - strand, within Pf1N1B4_4848 at 5357.625 kb on - strand at 5357.651 kb on + strand, within Pf1N1B4_4848 at 5357.651 kb on + strand, within Pf1N1B4_4848 at 5357.651 kb on + strand at 5357.652 kb on - strand, within Pf1N1B4_4848 at 5357.976 kb on + strand, within Pf1N1B4_4849 at 5358.306 kb on + strand, within Pf1N1B4_4849 at 5358.307 kb on - strand, within Pf1N1B4_4849 at 5358.712 kb on + strand at 5358.757 kb on + strand at 5358.780 kb on - strand at 5358.835 kb on + strand at 5358.835 kb on + strand at 5358.836 kb on - strand at 5358.836 kb on - strand at 5358.902 kb on - strand at 5358.902 kb on - strand at 5358.913 kb on - strand at 5359.079 kb on - strand, within Pf1N1B4_4850 at 5359.163 kb on + strand, within Pf1N1B4_4850 at 5359.163 kb on + strand, within Pf1N1B4_4850 at 5359.164 kb on - strand, within Pf1N1B4_4850 at 5359.164 kb on - strand at 5359.164 kb on - strand at 5359.165 kb on + strand, within Pf1N1B4_4850 at 5359.165 kb on + strand, within Pf1N1B4_4850 at 5359.166 kb on - strand, within Pf1N1B4_4850 at 5359.166 kb on - strand at 5359.166 kb on - strand, within Pf1N1B4_4850 at 5359.178 kb on - strand, within Pf1N1B4_4850 at 5359.244 kb on - strand at 5359.328 kb on - strand, within Pf1N1B4_4850 at 5359.328 kb on - strand, within Pf1N1B4_4850
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 5,356,994 - +0.1 5,357,342 + Pf1N1B4_4848 0.42 -2.6 5,357,343 - Pf1N1B4_4848 0.42 -0.7 5,357,343 - -0.6 5,357,348 + Pf1N1B4_4848 0.42 -0.6 5,357,349 - Pf1N1B4_4848 0.42 -0.3 5,357,387 + Pf1N1B4_4848 0.47 -0.6 5,357,445 - Pf1N1B4_4848 0.54 +0.4 5,357,528 + Pf1N1B4_4848 0.64 -3.2 5,357,529 - Pf1N1B4_4848 0.64 -2.9 5,357,625 - +1.0 5,357,651 + Pf1N1B4_4848 0.79 +0.1 5,357,651 + Pf1N1B4_4848 0.79 +1.8 5,357,651 + -1.6 5,357,652 - Pf1N1B4_4848 0.79 +1.8 5,357,976 + Pf1N1B4_4849 0.15 +1.0 5,358,306 + Pf1N1B4_4849 0.52 -1.1 5,358,307 - Pf1N1B4_4849 0.52 -0.9 5,358,712 + -1.3 5,358,757 + -1.2 5,358,780 - +0.0 5,358,835 + +0.4 5,358,835 + +0.4 5,358,836 - +0.2 5,358,836 - -0.6 5,358,902 - +0.4 5,358,902 - -3.4 5,358,913 - -1.0 5,359,079 - Pf1N1B4_4850 0.14 +0.4 5,359,163 + Pf1N1B4_4850 0.20 +0.0 5,359,163 + Pf1N1B4_4850 0.20 -0.2 5,359,164 - Pf1N1B4_4850 0.20 +0.8 5,359,164 - +0.4 5,359,164 - -1.6 5,359,165 + Pf1N1B4_4850 0.20 +1.4 5,359,165 + Pf1N1B4_4850 0.20 -0.6 5,359,166 - Pf1N1B4_4850 0.20 -0.2 5,359,166 - +0.4 5,359,166 - Pf1N1B4_4850 0.20 +0.2 5,359,178 - Pf1N1B4_4850 0.21 +0.2 5,359,244 - +1.0 5,359,328 - Pf1N1B4_4850 0.32 -0.3 5,359,328 - Pf1N1B4_4850 0.32 -0.6
Or see this region's nucleotide sequence