Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4540

Experiment: Cephalothin 2.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4538 and Pf1N1B4_4539 are separated by 228 nucleotidesPf1N1B4_4539 and Pf1N1B4_4540 are separated by 136 nucleotidesPf1N1B4_4540 and Pf1N1B4_4541 are separated by 18 nucleotidesPf1N1B4_4541 and Pf1N1B4_4542 overlap by 4 nucleotides Pf1N1B4_4538 - Uncharacterized protein ImpB, at 5,005,788 to 5,006,336 _4538 Pf1N1B4_4539 - Uncharacterized protein homologous to Paeru. PA2364, at 5,006,565 to 5,007,014 _4539 Pf1N1B4_4540 - Uncharacterized protein ImpJ/VasE, at 5,007,151 to 5,008,482 _4540 Pf1N1B4_4541 - Inner membrane protein DotU, at 5,008,501 to 5,009,268 _4541 Pf1N1B4_4542 - IcmF-related protein, at 5,009,265 to 5,012,888 _4542 Position (kb) 5007 5008 5009Strain fitness (log2 ratio) -2 -1 0 1at 5006.275 kb on + strand, within Pf1N1B4_4538at 5006.403 kb on - strandat 5006.511 kb on - strandat 5006.910 kb on + strand, within Pf1N1B4_4539at 5006.910 kb on + strandat 5006.910 kb on + strand, within Pf1N1B4_4539at 5006.910 kb on + strand, within Pf1N1B4_4539at 5006.910 kb on + strandat 5006.911 kb on - strand, within Pf1N1B4_4539at 5006.911 kb on - strand, within Pf1N1B4_4539at 5007.150 kb on + strandat 5007.150 kb on + strandat 5007.151 kb on - strandat 5007.161 kb on + strandat 5007.162 kb on - strandat 5007.268 kb on - strandat 5007.931 kb on + strandat 5007.932 kb on - strand, within Pf1N1B4_4540at 5007.932 kb on - strand, within Pf1N1B4_4540at 5008.500 kb on + strandat 5008.501 kb on - strandat 5008.501 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml
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5,006,275 + Pf1N1B4_4538 0.89 +0.2
5,006,403 - -0.7
5,006,511 - -1.1
5,006,910 + Pf1N1B4_4539 0.77 +0.8
5,006,910 + -0.5
5,006,910 + Pf1N1B4_4539 0.77 -0.2
5,006,910 + Pf1N1B4_4539 0.77 -0.5
5,006,910 + -0.5
5,006,911 - Pf1N1B4_4539 0.77 +0.8
5,006,911 - Pf1N1B4_4539 0.77 -0.5
5,007,150 + +0.0
5,007,150 + -1.5
5,007,151 - +1.1
5,007,161 + -1.1
5,007,162 - -0.5
5,007,268 - +1.1
5,007,931 + -1.5
5,007,932 - Pf1N1B4_4540 0.59 -0.8
5,007,932 - Pf1N1B4_4540 0.59 -0.8
5,008,500 + -0.2
5,008,501 - +0.5
5,008,501 - -2.5

Or see this region's nucleotide sequence