Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3684 and Pf1N1B4_3685 overlap by 1 nucleotides Pf1N1B4_3685 and Pf1N1B4_3686 are separated by 421 nucleotides
Pf1N1B4_3684 - Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5), at 4,094,056 to 4,095,222
_3684
Pf1N1B4_3685 - Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5), at 4,095,222 to 4,096,103
_3685
Pf1N1B4_3686 - Branched-chain amino acid transport system carrier protein, at 4,096,525 to 4,097,838
_3686
Position (kb)
4095
4096
4097 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4094.234 kb on + strand, within Pf1N1B4_3684 at 4094.305 kb on + strand at 4094.371 kb on - strand, within Pf1N1B4_3684 at 4094.380 kb on + strand at 4094.381 kb on - strand, within Pf1N1B4_3684 at 4094.600 kb on + strand at 4094.725 kb on + strand, within Pf1N1B4_3684 at 4094.737 kb on - strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.810 kb on - strand at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.813 kb on + strand, within Pf1N1B4_3684 at 4094.813 kb on + strand, within Pf1N1B4_3684 at 4094.813 kb on + strand, within Pf1N1B4_3684 at 4094.813 kb on + strand at 4094.813 kb on + strand, within Pf1N1B4_3684 at 4094.814 kb on - strand, within Pf1N1B4_3684 at 4094.814 kb on - strand, within Pf1N1B4_3684 at 4094.814 kb on - strand at 4094.934 kb on + strand, within Pf1N1B4_3684 at 4094.982 kb on + strand, within Pf1N1B4_3684 at 4095.220 kb on + strand at 4095.220 kb on + strand at 4095.240 kb on - strand at 4095.254 kb on + strand at 4095.254 kb on + strand at 4095.255 kb on - strand at 4095.284 kb on + strand at 4095.337 kb on + strand, within Pf1N1B4_3685 at 4095.403 kb on + strand at 4095.403 kb on + strand at 4095.435 kb on + strand, within Pf1N1B4_3685 at 4095.435 kb on + strand, within Pf1N1B4_3685 at 4095.435 kb on + strand, within Pf1N1B4_3685 at 4095.435 kb on + strand, within Pf1N1B4_3685 at 4095.435 kb on + strand, within Pf1N1B4_3685 at 4095.525 kb on - strand, within Pf1N1B4_3685 at 4095.551 kb on + strand, within Pf1N1B4_3685 at 4095.552 kb on - strand, within Pf1N1B4_3685 at 4095.587 kb on + strand, within Pf1N1B4_3685 at 4095.587 kb on + strand, within Pf1N1B4_3685 at 4095.696 kb on + strand, within Pf1N1B4_3685 at 4095.697 kb on - strand, within Pf1N1B4_3685 at 4095.697 kb on - strand, within Pf1N1B4_3685 at 4095.882 kb on + strand, within Pf1N1B4_3685 at 4095.883 kb on - strand, within Pf1N1B4_3685 at 4096.101 kb on + strand at 4096.101 kb on + strand at 4096.101 kb on + strand at 4096.101 kb on + strand at 4096.101 kb on + strand at 4096.101 kb on + strand at 4096.101 kb on + strand at 4096.102 kb on - strand at 4096.102 kb on - strand at 4096.102 kb on - strand at 4096.102 kb on - strand at 4096.102 kb on - strand at 4096.115 kb on - strand at 4096.115 kb on - strand at 4096.306 kb on + strand at 4096.307 kb on - strand at 4096.307 kb on - strand at 4096.307 kb on - strand at 4096.307 kb on - strand at 4096.311 kb on - strand at 4096.311 kb on - strand at 4096.311 kb on - strand at 4096.341 kb on + strand at 4096.354 kb on - strand at 4096.431 kb on - strand at 4096.446 kb on - strand at 4096.446 kb on - strand at 4096.507 kb on + strand at 4096.508 kb on - strand at 4096.515 kb on + strand at 4096.516 kb on - strand at 4096.516 kb on - strand at 4096.567 kb on - strand at 4096.638 kb on + strand at 4096.789 kb on + strand, within Pf1N1B4_3686 at 4096.790 kb on - strand, within Pf1N1B4_3686 at 4096.790 kb on - strand, within Pf1N1B4_3686 at 4096.894 kb on + strand, within Pf1N1B4_3686 at 4096.894 kb on + strand, within Pf1N1B4_3686 at 4096.895 kb on - strand, within Pf1N1B4_3686 at 4096.895 kb on - strand, within Pf1N1B4_3686 at 4096.896 kb on + strand, within Pf1N1B4_3686 at 4096.896 kb on + strand, within Pf1N1B4_3686 at 4096.897 kb on - strand, within Pf1N1B4_3686 at 4096.897 kb on - strand, within Pf1N1B4_3686 at 4096.907 kb on - strand, within Pf1N1B4_3686 at 4097.062 kb on + strand, within Pf1N1B4_3686 at 4097.063 kb on - strand, within Pf1N1B4_3686 at 4097.063 kb on - strand, within Pf1N1B4_3686 at 4097.063 kb on - strand, within Pf1N1B4_3686 at 4097.091 kb on + strand, within Pf1N1B4_3686 at 4097.091 kb on + strand, within Pf1N1B4_3686 at 4097.092 kb on - strand, within Pf1N1B4_3686 at 4097.092 kb on - strand, within Pf1N1B4_3686 at 4097.092 kb on - strand, within Pf1N1B4_3686 at 4097.101 kb on + strand, within Pf1N1B4_3686 at 4097.102 kb on - strand, within Pf1N1B4_3686
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 4,094,234 + Pf1N1B4_3684 0.15 -2.8 4,094,305 + +0.4 4,094,371 - Pf1N1B4_3684 0.27 -1.2 4,094,380 + -1.8 4,094,381 - Pf1N1B4_3684 0.28 -1.2 4,094,600 + +0.8 4,094,725 + Pf1N1B4_3684 0.57 -0.2 4,094,737 - Pf1N1B4_3684 0.58 +0.8 4,094,809 + Pf1N1B4_3684 0.65 -1.8 4,094,809 + Pf1N1B4_3684 0.65 -0.2 4,094,809 + Pf1N1B4_3684 0.65 -2.5 4,094,809 + Pf1N1B4_3684 0.65 -1.2 4,094,809 + Pf1N1B4_3684 0.65 +0.1 4,094,809 + -0.2 4,094,809 + Pf1N1B4_3684 0.65 -1.8 4,094,809 + Pf1N1B4_3684 0.65 +0.2 4,094,810 - Pf1N1B4_3684 0.65 -1.1 4,094,810 - Pf1N1B4_3684 0.65 +0.2 4,094,810 - Pf1N1B4_3684 0.65 +0.8 4,094,810 - Pf1N1B4_3684 0.65 -1.2 4,094,810 - -0.2 4,094,810 - Pf1N1B4_3684 0.65 -1.2 4,094,813 + Pf1N1B4_3684 0.65 -0.2 4,094,813 + Pf1N1B4_3684 0.65 +1.4 4,094,813 + Pf1N1B4_3684 0.65 -0.9 4,094,813 + -0.2 4,094,813 + Pf1N1B4_3684 0.65 +1.4 4,094,814 - Pf1N1B4_3684 0.65 -0.8 4,094,814 - Pf1N1B4_3684 0.65 -0.6 4,094,814 - +0.8 4,094,934 + Pf1N1B4_3684 0.75 +0.8 4,094,982 + Pf1N1B4_3684 0.79 -1.2 4,095,220 + -1.0 4,095,220 + -0.2 4,095,240 - -0.5 4,095,254 + +0.8 4,095,254 + +0.8 4,095,255 - +0.2 4,095,284 + +1.6 4,095,337 + Pf1N1B4_3685 0.13 -0.6 4,095,403 + +0.8 4,095,403 + -2.2 4,095,435 + Pf1N1B4_3685 0.24 -1.2 4,095,435 + Pf1N1B4_3685 0.24 -3.2 4,095,435 + Pf1N1B4_3685 0.24 -0.8 4,095,435 + Pf1N1B4_3685 0.24 +1.2 4,095,435 + Pf1N1B4_3685 0.24 -1.2 4,095,525 - Pf1N1B4_3685 0.34 -0.2 4,095,551 + Pf1N1B4_3685 0.37 +1.4 4,095,552 - Pf1N1B4_3685 0.37 -1.2 4,095,587 + Pf1N1B4_3685 0.41 -2.2 4,095,587 + Pf1N1B4_3685 0.41 -1.2 4,095,696 + Pf1N1B4_3685 0.54 -0.2 4,095,697 - Pf1N1B4_3685 0.54 -2.5 4,095,697 - Pf1N1B4_3685 0.54 -0.8 4,095,882 + Pf1N1B4_3685 0.75 -2.2 4,095,883 - Pf1N1B4_3685 0.75 -0.8 4,096,101 + -1.0 4,096,101 + -0.2 4,096,101 + +0.4 4,096,101 + -0.2 4,096,101 + -0.2 4,096,101 + -0.5 4,096,101 + -1.4 4,096,102 - -1.8 4,096,102 - -2.0 4,096,102 - +1.0 4,096,102 - -1.8 4,096,102 - +0.8 4,096,115 - -1.2 4,096,115 - -1.8 4,096,306 + +0.5 4,096,307 - +0.2 4,096,307 - -1.5 4,096,307 - +1.4 4,096,307 - -1.2 4,096,311 - +1.4 4,096,311 - -0.0 4,096,311 - -0.2 4,096,341 + -0.2 4,096,354 - -1.2 4,096,431 - -0.2 4,096,446 - -2.5 4,096,446 - -0.6 4,096,507 + -1.8 4,096,508 - -0.2 4,096,515 + -0.4 4,096,516 - +0.8 4,096,516 - -0.2 4,096,567 - +0.3 4,096,638 + +0.8 4,096,789 + Pf1N1B4_3686 0.20 -0.6 4,096,790 - Pf1N1B4_3686 0.20 -0.2 4,096,790 - Pf1N1B4_3686 0.20 -1.2 4,096,894 + Pf1N1B4_3686 0.28 +3.0 4,096,894 + Pf1N1B4_3686 0.28 -0.8 4,096,895 - Pf1N1B4_3686 0.28 -2.2 4,096,895 - Pf1N1B4_3686 0.28 +0.0 4,096,896 + Pf1N1B4_3686 0.28 -0.2 4,096,896 + Pf1N1B4_3686 0.28 +0.6 4,096,897 - Pf1N1B4_3686 0.28 +1.0 4,096,897 - Pf1N1B4_3686 0.28 +0.5 4,096,907 - Pf1N1B4_3686 0.29 +2.6 4,097,062 + Pf1N1B4_3686 0.41 -1.2 4,097,063 - Pf1N1B4_3686 0.41 -2.0 4,097,063 - Pf1N1B4_3686 0.41 -1.8 4,097,063 - Pf1N1B4_3686 0.41 +0.7 4,097,091 + Pf1N1B4_3686 0.43 -0.8 4,097,091 + Pf1N1B4_3686 0.43 -0.8 4,097,092 - Pf1N1B4_3686 0.43 -1.8 4,097,092 - Pf1N1B4_3686 0.43 -1.8 4,097,092 - Pf1N1B4_3686 0.43 -1.2 4,097,101 + Pf1N1B4_3686 0.44 +0.5 4,097,102 - Pf1N1B4_3686 0.44 -0.8
Or see this region's nucleotide sequence