Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3594 and Pf1N1B4_3595 are separated by 71 nucleotides Pf1N1B4_3595 and Pf1N1B4_3596 are separated by 82 nucleotides
Pf1N1B4_3594 - Flagellin protein FlaA, at 4,005,922 to 4,006,767
_3594
Pf1N1B4_3595 - Flagellin protein FlaG, at 4,006,839 to 4,007,204
_3595
Pf1N1B4_3596 - Flagellar hook-associated protein FliD, at 4,007,287 to 4,008,729
_3596
Position (kb)
4006
4007
4008 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4005.839 kb on + strand at 4005.839 kb on + strand at 4005.843 kb on + strand at 4005.843 kb on + strand at 4005.846 kb on + strand at 4005.930 kb on - strand at 4005.948 kb on + strand at 4005.948 kb on + strand at 4006.194 kb on + strand, within Pf1N1B4_3594 at 4006.195 kb on - strand, within Pf1N1B4_3594 at 4006.368 kb on - strand, within Pf1N1B4_3594 at 4006.503 kb on + strand, within Pf1N1B4_3594 at 4006.504 kb on - strand, within Pf1N1B4_3594 at 4006.504 kb on - strand, within Pf1N1B4_3594 at 4006.585 kb on - strand, within Pf1N1B4_3594 at 4006.766 kb on - strand at 4006.766 kb on - strand at 4006.768 kb on + strand at 4006.768 kb on + strand at 4006.768 kb on + strand at 4006.768 kb on + strand at 4006.768 kb on + strand at 4006.768 kb on + strand at 4006.768 kb on + strand at 4006.768 kb on + strand at 4006.768 kb on + strand at 4006.769 kb on - strand at 4006.819 kb on + strand at 4006.819 kb on + strand at 4006.820 kb on - strand at 4006.964 kb on + strand, within Pf1N1B4_3595 at 4007.159 kb on + strand at 4007.159 kb on + strand, within Pf1N1B4_3595 at 4007.160 kb on - strand, within Pf1N1B4_3595 at 4007.160 kb on - strand, within Pf1N1B4_3595 at 4007.218 kb on + strand at 4007.227 kb on + strand at 4007.227 kb on + strand at 4007.228 kb on - strand at 4007.246 kb on + strand at 4007.246 kb on + strand at 4007.247 kb on - strand at 4007.283 kb on - strand at 4007.283 kb on - strand at 4007.283 kb on - strand at 4007.343 kb on + strand at 4007.493 kb on + strand, within Pf1N1B4_3596 at 4007.494 kb on - strand, within Pf1N1B4_3596 at 4007.494 kb on - strand, within Pf1N1B4_3596 at 4007.519 kb on - strand, within Pf1N1B4_3596 at 4007.547 kb on - strand, within Pf1N1B4_3596 at 4007.587 kb on + strand, within Pf1N1B4_3596 at 4007.587 kb on + strand, within Pf1N1B4_3596 at 4007.587 kb on + strand, within Pf1N1B4_3596 at 4007.587 kb on + strand, within Pf1N1B4_3596 at 4007.587 kb on + strand, within Pf1N1B4_3596 at 4007.587 kb on + strand, within Pf1N1B4_3596 at 4007.588 kb on - strand, within Pf1N1B4_3596 at 4007.588 kb on - strand, within Pf1N1B4_3596 at 4007.588 kb on - strand at 4007.588 kb on - strand, within Pf1N1B4_3596 at 4007.588 kb on - strand, within Pf1N1B4_3596 at 4007.588 kb on - strand, within Pf1N1B4_3596 at 4007.588 kb on - strand, within Pf1N1B4_3596 at 4007.931 kb on + strand at 4007.932 kb on - strand, within Pf1N1B4_3596 at 4007.932 kb on - strand, within Pf1N1B4_3596 at 4007.932 kb on - strand, within Pf1N1B4_3596 at 4007.932 kb on - strand, within Pf1N1B4_3596 at 4008.009 kb on + strand, within Pf1N1B4_3596 at 4008.010 kb on - strand, within Pf1N1B4_3596 at 4008.010 kb on - strand, within Pf1N1B4_3596 at 4008.027 kb on + strand, within Pf1N1B4_3596 at 4008.027 kb on + strand, within Pf1N1B4_3596 at 4008.068 kb on + strand, within Pf1N1B4_3596 at 4008.068 kb on + strand, within Pf1N1B4_3596 at 4008.068 kb on + strand, within Pf1N1B4_3596 at 4008.068 kb on + strand, within Pf1N1B4_3596 at 4008.069 kb on - strand, within Pf1N1B4_3596 at 4008.069 kb on - strand, within Pf1N1B4_3596 at 4008.069 kb on - strand, within Pf1N1B4_3596 at 4008.139 kb on + strand, within Pf1N1B4_3596 at 4008.139 kb on + strand, within Pf1N1B4_3596 at 4008.140 kb on - strand, within Pf1N1B4_3596 at 4008.140 kb on - strand, within Pf1N1B4_3596 at 4008.140 kb on - strand, within Pf1N1B4_3596 at 4008.140 kb on - strand, within Pf1N1B4_3596 at 4008.140 kb on - strand, within Pf1N1B4_3596 at 4008.140 kb on - strand, within Pf1N1B4_3596
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 4,005,839 + -0.7 4,005,839 + +1.7 4,005,843 + +0.1 4,005,843 + +0.3 4,005,846 + +0.7 4,005,930 - -0.1 4,005,948 + -1.1 4,005,948 + +0.9 4,006,194 + Pf1N1B4_3594 0.32 -2.5 4,006,195 - Pf1N1B4_3594 0.32 -0.1 4,006,368 - Pf1N1B4_3594 0.53 -0.1 4,006,503 + Pf1N1B4_3594 0.69 +0.1 4,006,504 - Pf1N1B4_3594 0.69 +0.3 4,006,504 - Pf1N1B4_3594 0.69 +0.6 4,006,585 - Pf1N1B4_3594 0.78 -0.4 4,006,766 - -1.7 4,006,766 - +1.2 4,006,768 + +1.0 4,006,768 + +0.8 4,006,768 + -0.2 4,006,768 + -1.0 4,006,768 + +1.6 4,006,768 + +0.4 4,006,768 + -0.7 4,006,768 + +2.4 4,006,768 + -0.9 4,006,769 - +1.8 4,006,819 + +0.8 4,006,819 + +0.8 4,006,820 - -0.7 4,006,964 + Pf1N1B4_3595 0.34 -0.2 4,007,159 + +0.8 4,007,159 + Pf1N1B4_3595 0.87 -1.7 4,007,160 - Pf1N1B4_3595 0.88 -1.7 4,007,160 - Pf1N1B4_3595 0.88 +0.2 4,007,218 + +1.4 4,007,227 + -1.2 4,007,227 + -1.2 4,007,228 - +0.1 4,007,246 + +2.4 4,007,246 + +1.4 4,007,247 - -0.2 4,007,283 - +2.7 4,007,283 - -1.4 4,007,283 - -1.5 4,007,343 + -0.4 4,007,493 + Pf1N1B4_3596 0.14 -1.7 4,007,494 - Pf1N1B4_3596 0.14 -0.2 4,007,494 - Pf1N1B4_3596 0.14 +0.2 4,007,519 - Pf1N1B4_3596 0.16 -0.3 4,007,547 - Pf1N1B4_3596 0.18 -0.2 4,007,587 + Pf1N1B4_3596 0.21 -0.4 4,007,587 + Pf1N1B4_3596 0.21 -0.7 4,007,587 + Pf1N1B4_3596 0.21 -2.2 4,007,587 + Pf1N1B4_3596 0.21 -0.2 4,007,587 + Pf1N1B4_3596 0.21 +0.3 4,007,587 + Pf1N1B4_3596 0.21 +0.8 4,007,588 - Pf1N1B4_3596 0.21 +0.7 4,007,588 - Pf1N1B4_3596 0.21 +0.3 4,007,588 - -0.2 4,007,588 - Pf1N1B4_3596 0.21 -0.5 4,007,588 - Pf1N1B4_3596 0.21 -0.9 4,007,588 - Pf1N1B4_3596 0.21 +1.7 4,007,588 - Pf1N1B4_3596 0.21 -1.0 4,007,931 + +0.8 4,007,932 - Pf1N1B4_3596 0.45 -2.5 4,007,932 - Pf1N1B4_3596 0.45 +0.0 4,007,932 - Pf1N1B4_3596 0.45 -0.2 4,007,932 - Pf1N1B4_3596 0.45 -0.9 4,008,009 + Pf1N1B4_3596 0.50 +1.7 4,008,010 - Pf1N1B4_3596 0.50 -0.8 4,008,010 - Pf1N1B4_3596 0.50 -0.6 4,008,027 + Pf1N1B4_3596 0.51 -0.5 4,008,027 + Pf1N1B4_3596 0.51 -1.2 4,008,068 + Pf1N1B4_3596 0.54 +0.1 4,008,068 + Pf1N1B4_3596 0.54 +1.1 4,008,068 + Pf1N1B4_3596 0.54 -0.7 4,008,068 + Pf1N1B4_3596 0.54 +1.2 4,008,069 - Pf1N1B4_3596 0.54 -0.6 4,008,069 - Pf1N1B4_3596 0.54 -0.4 4,008,069 - Pf1N1B4_3596 0.54 +3.3 4,008,139 + Pf1N1B4_3596 0.59 -0.9 4,008,139 + Pf1N1B4_3596 0.59 -0.7 4,008,140 - Pf1N1B4_3596 0.59 -0.2 4,008,140 - Pf1N1B4_3596 0.59 -0.1 4,008,140 - Pf1N1B4_3596 0.59 -2.7 4,008,140 - Pf1N1B4_3596 0.59 +0.3 4,008,140 - Pf1N1B4_3596 0.59 -0.5 4,008,140 - Pf1N1B4_3596 0.59 +0.8
Or see this region's nucleotide sequence