Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3539 and Pf1N1B4_3540 are separated by 72 nucleotides Pf1N1B4_3540 and Pf1N1B4_3541 are separated by 99 nucleotides Pf1N1B4_3541 and Pf1N1B4_3542 are separated by 191 nucleotides
Pf1N1B4_3539 - Ser/Thr protein phosphatase family protein, UDP-2,3-diacylglucosamine hydrolase (EC 3.6.1.-) homolog, at 3,946,072 to 3,946,887
_3539
Pf1N1B4_3540 - Metal-dependent phosphohydrolase, HD subdomain, at 3,946,960 to 3,947,550
_3540
Pf1N1B4_3541 - FIG027190: Putative transmembrane protein, at 3,947,650 to 3,947,961
_3541
Pf1N1B4_3542 - Transcriptional regulator, AraC family, at 3,948,153 to 3,949,181
_3542
Position (kb)
3947
3948 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3946.747 kb on + strand, within Pf1N1B4_3539 at 3946.747 kb on + strand at 3946.819 kb on + strand at 3946.819 kb on + strand at 3946.866 kb on + strand at 3946.866 kb on + strand at 3946.898 kb on + strand at 3946.898 kb on + strand at 3946.899 kb on - strand at 3946.899 kb on - strand at 3946.899 kb on - strand at 3946.958 kb on + strand at 3946.959 kb on - strand at 3946.959 kb on - strand at 3946.959 kb on - strand at 3946.973 kb on + strand at 3946.974 kb on - strand at 3946.974 kb on - strand at 3947.123 kb on + strand at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.190 kb on - strand, within Pf1N1B4_3540 at 3947.282 kb on + strand, within Pf1N1B4_3540 at 3947.330 kb on + strand, within Pf1N1B4_3540 at 3947.330 kb on + strand at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand at 3947.425 kb on + strand, within Pf1N1B4_3540 at 3947.523 kb on + strand at 3947.524 kb on - strand at 3947.592 kb on + strand at 3947.652 kb on - strand at 3947.652 kb on - strand at 3947.684 kb on + strand, within Pf1N1B4_3541 at 3947.685 kb on - strand, within Pf1N1B4_3541 at 3947.685 kb on - strand, within Pf1N1B4_3541 at 3947.778 kb on + strand at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.913 kb on - strand, within Pf1N1B4_3541 at 3948.015 kb on + strand at 3948.015 kb on + strand at 3948.016 kb on - strand at 3948.019 kb on + strand at 3948.020 kb on - strand at 3948.020 kb on - strand at 3948.057 kb on - strand at 3948.068 kb on + strand at 3948.069 kb on - strand at 3948.109 kb on + strand at 3948.113 kb on - strand at 3948.225 kb on + strand at 3948.483 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.649 kb on - strand, within Pf1N1B4_3542 at 3948.690 kb on + strand, within Pf1N1B4_3542 at 3948.691 kb on - strand at 3948.691 kb on - strand, within Pf1N1B4_3542 at 3948.786 kb on + strand, within Pf1N1B4_3542 at 3948.787 kb on - strand, within Pf1N1B4_3542 at 3948.838 kb on + strand at 3948.838 kb on + strand, within Pf1N1B4_3542 at 3948.875 kb on + strand, within Pf1N1B4_3542
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 3,946,747 + Pf1N1B4_3539 0.83 +0.1 3,946,747 + -0.1 3,946,819 + +1.5 3,946,819 + -0.7 3,946,866 + -0.9 3,946,866 + +1.2 3,946,898 + +0.9 3,946,898 + +0.3 3,946,899 - -0.9 3,946,899 - +1.2 3,946,899 - -0.1 3,946,958 + -0.1 3,946,959 - +0.7 3,946,959 - +1.5 3,946,959 - +0.7 3,946,973 + +0.7 3,946,974 - +0.5 3,946,974 - -0.1 3,947,123 + -0.1 3,947,186 + Pf1N1B4_3540 0.38 -1.1 3,947,186 + Pf1N1B4_3540 0.38 +0.3 3,947,186 + Pf1N1B4_3540 0.38 +1.5 3,947,187 - Pf1N1B4_3540 0.38 -0.1 3,947,187 - Pf1N1B4_3540 0.38 +0.2 3,947,187 - Pf1N1B4_3540 0.38 -0.1 3,947,187 - Pf1N1B4_3540 0.38 +1.5 3,947,190 - Pf1N1B4_3540 0.39 +1.7 3,947,282 + Pf1N1B4_3540 0.54 +0.9 3,947,330 + Pf1N1B4_3540 0.63 +1.9 3,947,330 + +1.5 3,947,331 - Pf1N1B4_3540 0.63 +1.2 3,947,331 - Pf1N1B4_3540 0.63 -0.9 3,947,331 - Pf1N1B4_3540 0.63 -0.7 3,947,331 - Pf1N1B4_3540 0.63 -0.1 3,947,331 - Pf1N1B4_3540 0.63 +1.1 3,947,331 - Pf1N1B4_3540 0.63 -0.5 3,947,331 - +0.5 3,947,425 + Pf1N1B4_3540 0.79 +1.5 3,947,523 + -1.5 3,947,524 - +0.9 3,947,592 + -0.5 3,947,652 - +0.9 3,947,652 - -0.7 3,947,684 + Pf1N1B4_3541 0.11 +1.0 3,947,685 - Pf1N1B4_3541 0.11 -0.5 3,947,685 - Pf1N1B4_3541 0.11 -0.1 3,947,778 + +1.5 3,947,778 + Pf1N1B4_3541 0.41 -0.1 3,947,778 + Pf1N1B4_3541 0.41 +0.5 3,947,778 + Pf1N1B4_3541 0.41 -1.1 3,947,779 - Pf1N1B4_3541 0.41 -0.7 3,947,779 - Pf1N1B4_3541 0.41 +0.1 3,947,779 - Pf1N1B4_3541 0.41 -0.7 3,947,779 - Pf1N1B4_3541 0.41 +0.9 3,947,913 - Pf1N1B4_3541 0.84 -1.5 3,948,015 + +2.5 3,948,015 + +0.5 3,948,016 - -1.7 3,948,019 + +0.3 3,948,020 - +1.9 3,948,020 - -1.7 3,948,057 - -1.1 3,948,068 + +0.9 3,948,069 - -1.6 3,948,109 + +0.9 3,948,113 - -0.3 3,948,225 + +1.5 3,948,483 + Pf1N1B4_3542 0.32 -0.3 3,948,648 + Pf1N1B4_3542 0.48 -0.1 3,948,648 + Pf1N1B4_3542 0.48 +0.9 3,948,648 + Pf1N1B4_3542 0.48 +0.5 3,948,648 + -1.1 3,948,648 + Pf1N1B4_3542 0.48 -0.7 3,948,648 + Pf1N1B4_3542 0.48 +0.1 3,948,648 + Pf1N1B4_3542 0.48 -0.3 3,948,648 + Pf1N1B4_3542 0.48 +0.9 3,948,649 - Pf1N1B4_3542 0.48 -2.9 3,948,690 + Pf1N1B4_3542 0.52 -1.5 3,948,691 - +0.9 3,948,691 - Pf1N1B4_3542 0.52 -1.1 3,948,786 + Pf1N1B4_3542 0.62 +0.1 3,948,787 - Pf1N1B4_3542 0.62 +0.5 3,948,838 + -0.1 3,948,838 + Pf1N1B4_3542 0.67 +0.4 3,948,875 + Pf1N1B4_3542 0.70 +0.8
Or see this region's nucleotide sequence