Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3231 and Pf1N1B4_3232 are separated by 283 nucleotides Pf1N1B4_3232 and Pf1N1B4_3233 overlap by 1 nucleotides Pf1N1B4_3233 and Pf1N1B4_3234 overlap by 1 nucleotides
Pf1N1B4_3231 - 4-hydroxybenzoate transporter, at 3,617,619 to 3,618,965
_3231
Pf1N1B4_3232 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6); Glutaconate CoA-transferase subunit A (EC 2.8.3.12), at 3,619,249 to 3,620,106
_3232
Pf1N1B4_3233 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6); Glutaconate CoA-transferase subunit B (EC 2.8.3.12), at 3,620,106 to 3,620,885
_3233
Pf1N1B4_3234 - Beta-ketoadipyl CoA thiolase (EC 2.3.1.-), at 3,620,885 to 3,622,087
_3234
Position (kb)
3619
3620
3621 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3618.329 kb on + strand, within Pf1N1B4_3231 at 3618.329 kb on + strand, within Pf1N1B4_3231 at 3618.329 kb on + strand, within Pf1N1B4_3231 at 3618.330 kb on - strand, within Pf1N1B4_3231 at 3618.330 kb on - strand, within Pf1N1B4_3231 at 3618.330 kb on - strand, within Pf1N1B4_3231 at 3618.399 kb on + strand at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.399 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.432 kb on + strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.433 kb on - strand, within Pf1N1B4_3231 at 3618.456 kb on + strand, within Pf1N1B4_3231 at 3618.457 kb on - strand, within Pf1N1B4_3231 at 3618.625 kb on + strand, within Pf1N1B4_3231 at 3618.625 kb on + strand, within Pf1N1B4_3231 at 3618.625 kb on + strand, within Pf1N1B4_3231 at 3618.626 kb on - strand, within Pf1N1B4_3231 at 3618.646 kb on - strand, within Pf1N1B4_3231 at 3618.759 kb on + strand, within Pf1N1B4_3231 at 3619.170 kb on + strand at 3619.212 kb on - strand at 3619.228 kb on - strand at 3619.417 kb on + strand, within Pf1N1B4_3232 at 3619.418 kb on - strand, within Pf1N1B4_3232 at 3619.418 kb on - strand, within Pf1N1B4_3232 at 3619.418 kb on - strand, within Pf1N1B4_3232 at 3619.579 kb on + strand, within Pf1N1B4_3232 at 3619.580 kb on - strand, within Pf1N1B4_3232 at 3619.580 kb on - strand, within Pf1N1B4_3232 at 3619.624 kb on + strand, within Pf1N1B4_3232 at 3619.920 kb on - strand, within Pf1N1B4_3232 at 3619.978 kb on + strand, within Pf1N1B4_3232 at 3619.978 kb on + strand, within Pf1N1B4_3232 at 3619.978 kb on + strand at 3619.979 kb on - strand, within Pf1N1B4_3232 at 3619.979 kb on - strand, within Pf1N1B4_3232 at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.104 kb on + strand at 3620.104 kb on + strand at 3620.104 kb on + strand at 3620.105 kb on - strand at 3620.105 kb on - strand at 3620.105 kb on - strand at 3620.112 kb on + strand at 3620.112 kb on + strand at 3620.112 kb on + strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.239 kb on + strand at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.284 kb on + strand, within Pf1N1B4_3233 at 3620.455 kb on - strand, within Pf1N1B4_3233 at 3620.455 kb on - strand, within Pf1N1B4_3233 at 3620.524 kb on + strand, within Pf1N1B4_3233 at 3620.525 kb on - strand, within Pf1N1B4_3233 at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.900 kb on - strand at 3620.900 kb on - strand at 3620.900 kb on - strand at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.085 kb on - strand, within Pf1N1B4_3234
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 3,618,329 + Pf1N1B4_3231 0.53 -0.5 3,618,329 + Pf1N1B4_3231 0.53 -2.0 3,618,329 + Pf1N1B4_3231 0.53 +0.7 3,618,330 - Pf1N1B4_3231 0.53 -0.2 3,618,330 - Pf1N1B4_3231 0.53 +0.8 3,618,330 - Pf1N1B4_3231 0.53 -0.3 3,618,399 + +1.6 3,618,399 + Pf1N1B4_3231 0.58 -1.0 3,618,399 + Pf1N1B4_3231 0.58 +1.2 3,618,399 + Pf1N1B4_3231 0.58 -1.4 3,618,399 + Pf1N1B4_3231 0.58 +1.3 3,618,399 + Pf1N1B4_3231 0.58 -1.6 3,618,399 + Pf1N1B4_3231 0.58 -2.0 3,618,432 + Pf1N1B4_3231 0.60 -1.2 3,618,432 + Pf1N1B4_3231 0.60 +0.4 3,618,432 + Pf1N1B4_3231 0.60 +0.5 3,618,432 + Pf1N1B4_3231 0.60 -0.0 3,618,432 + Pf1N1B4_3231 0.60 +0.4 3,618,432 + Pf1N1B4_3231 0.60 -0.3 3,618,432 + Pf1N1B4_3231 0.60 -0.6 3,618,433 - Pf1N1B4_3231 0.60 +1.7 3,618,433 - Pf1N1B4_3231 0.60 -0.0 3,618,433 - Pf1N1B4_3231 0.60 -0.0 3,618,433 - Pf1N1B4_3231 0.60 +1.6 3,618,433 - Pf1N1B4_3231 0.60 -1.6 3,618,456 + Pf1N1B4_3231 0.62 +1.6 3,618,457 - Pf1N1B4_3231 0.62 +1.0 3,618,625 + Pf1N1B4_3231 0.75 +0.4 3,618,625 + Pf1N1B4_3231 0.75 -0.3 3,618,625 + Pf1N1B4_3231 0.75 -0.8 3,618,626 - Pf1N1B4_3231 0.75 +0.1 3,618,646 - Pf1N1B4_3231 0.76 -0.7 3,618,759 + Pf1N1B4_3231 0.85 -0.2 3,619,170 + -1.0 3,619,212 - -1.0 3,619,228 - -2.6 3,619,417 + Pf1N1B4_3232 0.20 +1.6 3,619,418 - Pf1N1B4_3232 0.20 +0.9 3,619,418 - Pf1N1B4_3232 0.20 -0.0 3,619,418 - Pf1N1B4_3232 0.20 +2.3 3,619,579 + Pf1N1B4_3232 0.38 -0.4 3,619,580 - Pf1N1B4_3232 0.39 -0.6 3,619,580 - Pf1N1B4_3232 0.39 +1.4 3,619,624 + Pf1N1B4_3232 0.44 +2.0 3,619,920 - Pf1N1B4_3232 0.78 -0.2 3,619,978 + Pf1N1B4_3232 0.85 +0.2 3,619,978 + Pf1N1B4_3232 0.85 +2.0 3,619,978 + -1.3 3,619,979 - Pf1N1B4_3232 0.85 -0.8 3,619,979 - Pf1N1B4_3232 0.85 +1.4 3,620,038 + -1.3 3,620,038 + +1.2 3,620,038 + +0.0 3,620,038 + -1.6 3,620,038 + +1.2 3,620,038 + +0.4 3,620,038 + -0.0 3,620,039 - +2.0 3,620,039 - -0.5 3,620,039 - +1.0 3,620,039 - -2.3 3,620,104 + -2.0 3,620,104 + +1.6 3,620,104 + -0.0 3,620,105 - -1.6 3,620,105 - -1.8 3,620,105 - +0.3 3,620,112 + -1.0 3,620,112 + -0.0 3,620,112 + +2.6 3,620,113 - -1.6 3,620,113 - -1.6 3,620,113 - +1.0 3,620,113 - -1.6 3,620,113 - -0.0 3,620,113 - -1.6 3,620,113 - -0.0 3,620,239 + Pf1N1B4_3233 0.17 +0.7 3,620,239 + +2.3 3,620,239 + Pf1N1B4_3233 0.17 +0.3 3,620,239 + Pf1N1B4_3233 0.17 +0.4 3,620,240 - Pf1N1B4_3233 0.17 -0.7 3,620,240 - Pf1N1B4_3233 0.17 +1.3 3,620,240 - Pf1N1B4_3233 0.17 -0.3 3,620,250 + Pf1N1B4_3233 0.18 -1.0 3,620,250 + Pf1N1B4_3233 0.18 -1.2 3,620,250 + Pf1N1B4_3233 0.18 -0.0 3,620,250 + Pf1N1B4_3233 0.18 -1.6 3,620,250 + Pf1N1B4_3233 0.18 +1.6 3,620,251 - Pf1N1B4_3233 0.19 -0.0 3,620,251 - Pf1N1B4_3233 0.19 +0.4 3,620,251 - Pf1N1B4_3233 0.19 -0.6 3,620,284 + Pf1N1B4_3233 0.23 -0.0 3,620,455 - Pf1N1B4_3233 0.45 -0.4 3,620,455 - Pf1N1B4_3233 0.45 +0.7 3,620,524 + Pf1N1B4_3233 0.54 +0.3 3,620,525 - Pf1N1B4_3233 0.54 -2.3 3,620,897 + -0.3 3,620,897 + -1.3 3,620,897 + -1.6 3,620,897 + +0.7 3,620,897 + -0.3 3,620,897 + -0.0 3,620,897 + -0.9 3,620,897 + +1.3 3,620,898 - +0.6 3,620,898 - +0.3 3,620,898 - -1.6 3,620,898 - -1.6 3,620,899 + -0.6 3,620,899 + -0.1 3,620,899 + +1.8 3,620,899 + +0.2 3,620,899 + -1.0 3,620,899 + -1.6 3,620,900 - +0.7 3,620,900 - +1.3 3,620,900 - -0.0 3,621,084 + Pf1N1B4_3234 0.17 +1.3 3,621,084 + Pf1N1B4_3234 0.17 +1.0 3,621,084 + Pf1N1B4_3234 0.17 +1.0 3,621,085 - Pf1N1B4_3234 0.17 +1.0
Or see this region's nucleotide sequence