Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3025 and Pf1N1B4_3026 overlap by 11 nucleotides Pf1N1B4_3026 and Pf1N1B4_3027 are separated by 1 nucleotides Pf1N1B4_3027 and Pf1N1B4_3028 are separated by 109 nucleotides
Pf1N1B4_3025 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,402,554 to 3,403,378
_3025
Pf1N1B4_3026 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,403,368 to 3,404,315
_3026
Pf1N1B4_3027 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 3,404,317 to 3,405,435
_3027
Pf1N1B4_3028 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 3,405,545 to 3,406,696
_3028
Position (kb)
3404
3405
3406 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3403.335 kb on - strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.463 kb on + strand, within Pf1N1B4_3026 at 3403.489 kb on + strand, within Pf1N1B4_3026 at 3403.490 kb on - strand, within Pf1N1B4_3026 at 3403.665 kb on + strand, within Pf1N1B4_3026 at 3403.666 kb on - strand, within Pf1N1B4_3026 at 3403.666 kb on - strand, within Pf1N1B4_3026 at 3403.732 kb on + strand, within Pf1N1B4_3026 at 3403.733 kb on - strand, within Pf1N1B4_3026 at 3403.733 kb on - strand, within Pf1N1B4_3026 at 3403.888 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.936 kb on + strand, within Pf1N1B4_3026 at 3403.967 kb on - strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.982 kb on - strand, within Pf1N1B4_3026 at 3403.982 kb on - strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.141 kb on - strand at 3404.179 kb on + strand, within Pf1N1B4_3026 at 3404.179 kb on + strand, within Pf1N1B4_3026 at 3404.227 kb on + strand at 3404.227 kb on + strand at 3404.227 kb on + strand at 3404.228 kb on - strand at 3404.228 kb on - strand at 3404.245 kb on + strand at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.688 kb on - strand, within Pf1N1B4_3027 at 3404.964 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.077 kb on - strand, within Pf1N1B4_3027 at 3405.077 kb on - strand, within Pf1N1B4_3027 at 3405.117 kb on + strand, within Pf1N1B4_3027 at 3405.117 kb on + strand at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.168 kb on + strand, within Pf1N1B4_3027 at 3405.560 kb on - strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.570 kb on + strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.571 kb on - strand at 3405.590 kb on - strand at 3405.591 kb on + strand at 3405.591 kb on + strand at 3405.591 kb on + strand at 3405.592 kb on - strand at 3405.592 kb on - strand at 3405.703 kb on + strand, within Pf1N1B4_3028 at 3405.704 kb on - strand, within Pf1N1B4_3028 at 3405.792 kb on + strand at 3405.793 kb on - strand, within Pf1N1B4_3028 at 3406.038 kb on + strand, within Pf1N1B4_3028 at 3406.039 kb on - strand, within Pf1N1B4_3028 at 3406.039 kb on - strand, within Pf1N1B4_3028 at 3406.059 kb on + strand, within Pf1N1B4_3028 at 3406.101 kb on + strand, within Pf1N1B4_3028 at 3406.101 kb on + strand, within Pf1N1B4_3028 at 3406.101 kb on + strand, within Pf1N1B4_3028 at 3406.122 kb on + strand, within Pf1N1B4_3028 at 3406.122 kb on + strand, within Pf1N1B4_3028 at 3406.122 kb on + strand, within Pf1N1B4_3028 at 3406.122 kb on + strand, within Pf1N1B4_3028 at 3406.123 kb on - strand, within Pf1N1B4_3028 at 3406.123 kb on - strand, within Pf1N1B4_3028 at 3406.123 kb on - strand, within Pf1N1B4_3028 at 3406.137 kb on + strand, within Pf1N1B4_3028 at 3406.138 kb on - strand, within Pf1N1B4_3028 at 3406.138 kb on - strand, within Pf1N1B4_3028 at 3406.138 kb on - strand, within Pf1N1B4_3028 at 3406.210 kb on + strand at 3406.210 kb on + strand, within Pf1N1B4_3028 at 3406.211 kb on - strand at 3406.211 kb on - strand, within Pf1N1B4_3028 at 3406.239 kb on + strand, within Pf1N1B4_3028 at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand at 3406.240 kb on - strand at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.240 kb on - strand at 3406.240 kb on - strand, within Pf1N1B4_3028 at 3406.279 kb on - strand, within Pf1N1B4_3028 at 3406.297 kb on + strand at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand, within Pf1N1B4_3028 at 3406.297 kb on + strand at 3406.298 kb on - strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.299 kb on + strand, within Pf1N1B4_3028 at 3406.300 kb on - strand at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.300 kb on - strand, within Pf1N1B4_3028 at 3406.358 kb on - strand, within Pf1N1B4_3028 at 3406.358 kb on - strand, within Pf1N1B4_3028
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 3,403,335 - +1.1 3,403,366 + +0.3 3,403,366 + +0.1 3,403,366 + +1.4 3,403,367 - -0.9 3,403,367 - +1.1 3,403,367 - +0.7 3,403,411 + +1.1 3,403,411 + -0.9 3,403,411 + +1.1 3,403,411 + +0.7 3,403,411 + +1.1 3,403,411 + +0.7 3,403,412 - +0.1 3,403,412 - -0.9 3,403,412 - -0.5 3,403,412 - -1.2 3,403,463 + Pf1N1B4_3026 0.10 -0.5 3,403,489 + Pf1N1B4_3026 0.13 +1.7 3,403,490 - Pf1N1B4_3026 0.13 -0.4 3,403,665 + Pf1N1B4_3026 0.31 -0.4 3,403,666 - Pf1N1B4_3026 0.31 -0.3 3,403,666 - Pf1N1B4_3026 0.31 -1.4 3,403,732 + Pf1N1B4_3026 0.38 +0.7 3,403,733 - Pf1N1B4_3026 0.39 +1.3 3,403,733 - Pf1N1B4_3026 0.39 -0.1 3,403,888 + Pf1N1B4_3026 0.55 -0.6 3,403,903 + Pf1N1B4_3026 0.56 -0.9 3,403,903 + Pf1N1B4_3026 0.56 +0.5 3,403,903 + Pf1N1B4_3026 0.56 -1.5 3,403,904 - Pf1N1B4_3026 0.57 -0.4 3,403,904 - Pf1N1B4_3026 0.57 -0.3 3,403,904 - Pf1N1B4_3026 0.57 -1.3 3,403,936 + Pf1N1B4_3026 0.60 -0.1 3,403,967 - Pf1N1B4_3026 0.63 -0.9 3,403,978 + Pf1N1B4_3026 0.64 +1.1 3,403,978 + Pf1N1B4_3026 0.64 -1.9 3,403,978 + Pf1N1B4_3026 0.64 -0.2 3,403,978 + Pf1N1B4_3026 0.64 +0.1 3,403,978 + Pf1N1B4_3026 0.64 +1.1 3,403,978 + Pf1N1B4_3026 0.64 -1.9 3,403,978 + Pf1N1B4_3026 0.64 +0.7 3,403,978 + -1.5 3,403,979 - Pf1N1B4_3026 0.64 -0.6 3,403,979 - Pf1N1B4_3026 0.64 -1.5 3,403,979 - Pf1N1B4_3026 0.64 -0.1 3,403,979 - Pf1N1B4_3026 0.64 -1.1 3,403,979 - Pf1N1B4_3026 0.64 +0.1 3,403,982 - Pf1N1B4_3026 0.65 +1.1 3,403,982 - Pf1N1B4_3026 0.65 +0.1 3,404,140 + Pf1N1B4_3026 0.81 +1.1 3,404,140 + Pf1N1B4_3026 0.81 +1.1 3,404,140 + Pf1N1B4_3026 0.81 -0.1 3,404,140 + Pf1N1B4_3026 0.81 -0.5 3,404,141 - +1.7 3,404,179 + Pf1N1B4_3026 0.86 -0.5 3,404,179 + Pf1N1B4_3026 0.86 -0.7 3,404,227 + +1.4 3,404,227 + -0.5 3,404,227 + -2.4 3,404,228 - +0.1 3,404,228 - -1.3 3,404,245 + -1.9 3,404,687 + Pf1N1B4_3027 0.33 -2.5 3,404,687 + Pf1N1B4_3027 0.33 -2.2 3,404,687 + Pf1N1B4_3027 0.33 -0.9 3,404,687 + Pf1N1B4_3027 0.33 -0.9 3,404,688 - Pf1N1B4_3027 0.33 +1.1 3,404,964 + Pf1N1B4_3027 0.58 -0.5 3,405,076 + Pf1N1B4_3027 0.68 +0.1 3,405,076 + Pf1N1B4_3027 0.68 -0.7 3,405,076 + Pf1N1B4_3027 0.68 -0.5 3,405,077 - Pf1N1B4_3027 0.68 -1.2 3,405,077 - Pf1N1B4_3027 0.68 -2.2 3,405,117 + Pf1N1B4_3027 0.71 -0.5 3,405,117 + -0.9 3,405,118 - Pf1N1B4_3027 0.72 -0.2 3,405,118 - Pf1N1B4_3027 0.72 -1.1 3,405,118 - Pf1N1B4_3027 0.72 -0.9 3,405,118 - Pf1N1B4_3027 0.72 -0.5 3,405,168 + Pf1N1B4_3027 0.76 -1.5 3,405,560 - +1.4 3,405,570 + -1.5 3,405,570 + +0.8 3,405,570 + +1.1 3,405,570 + -2.5 3,405,570 + +0.4 3,405,570 + +2.1 3,405,570 + +0.9 3,405,571 - -1.5 3,405,571 - -2.2 3,405,571 - -0.9 3,405,571 - +0.5 3,405,571 - +0.1 3,405,571 - +0.7 3,405,571 - +0.1 3,405,571 - +2.4 3,405,571 - +0.1 3,405,590 - +1.1 3,405,591 + -0.5 3,405,591 + -2.5 3,405,591 + -0.4 3,405,592 - +0.1 3,405,592 - +0.1 3,405,703 + Pf1N1B4_3028 0.14 +0.8 3,405,704 - Pf1N1B4_3028 0.14 -1.1 3,405,792 + +0.1 3,405,793 - Pf1N1B4_3028 0.22 +0.1 3,406,038 + Pf1N1B4_3028 0.43 -0.5 3,406,039 - Pf1N1B4_3028 0.43 +0.8 3,406,039 - Pf1N1B4_3028 0.43 +1.5 3,406,059 + Pf1N1B4_3028 0.45 +1.3 3,406,101 + Pf1N1B4_3028 0.48 -0.2 3,406,101 + Pf1N1B4_3028 0.48 -0.3 3,406,101 + Pf1N1B4_3028 0.48 -1.5 3,406,122 + Pf1N1B4_3028 0.50 -0.6 3,406,122 + Pf1N1B4_3028 0.50 +0.1 3,406,122 + Pf1N1B4_3028 0.50 +0.5 3,406,122 + Pf1N1B4_3028 0.50 -2.7 3,406,123 - Pf1N1B4_3028 0.50 +0.5 3,406,123 - Pf1N1B4_3028 0.50 +0.3 3,406,123 - Pf1N1B4_3028 0.50 +0.2 3,406,137 + Pf1N1B4_3028 0.51 +0.1 3,406,138 - Pf1N1B4_3028 0.51 -2.7 3,406,138 - Pf1N1B4_3028 0.51 -0.9 3,406,138 - Pf1N1B4_3028 0.51 +1.1 3,406,210 + -0.3 3,406,210 + Pf1N1B4_3028 0.58 -0.8 3,406,211 - -1.5 3,406,211 - Pf1N1B4_3028 0.58 -1.5 3,406,239 + Pf1N1B4_3028 0.60 -0.9 3,406,240 - Pf1N1B4_3028 0.60 -1.5 3,406,240 - Pf1N1B4_3028 0.60 +0.1 3,406,240 - -0.5 3,406,240 - -0.9 3,406,240 - Pf1N1B4_3028 0.60 -2.5 3,406,240 - Pf1N1B4_3028 0.60 -1.5 3,406,240 - Pf1N1B4_3028 0.60 +2.4 3,406,240 - +0.7 3,406,240 - Pf1N1B4_3028 0.60 -0.0 3,406,279 - Pf1N1B4_3028 0.64 +0.1 3,406,297 + +1.1 3,406,297 + Pf1N1B4_3028 0.65 +2.1 3,406,297 + Pf1N1B4_3028 0.65 -0.5 3,406,297 + Pf1N1B4_3028 0.65 -1.5 3,406,297 + Pf1N1B4_3028 0.65 -0.5 3,406,297 + Pf1N1B4_3028 0.65 +0.5 3,406,297 + Pf1N1B4_3028 0.65 +0.4 3,406,297 + Pf1N1B4_3028 0.65 -1.1 3,406,297 + +0.1 3,406,298 - Pf1N1B4_3028 0.65 +0.1 3,406,299 + Pf1N1B4_3028 0.65 +0.7 3,406,299 + Pf1N1B4_3028 0.65 -1.2 3,406,299 + Pf1N1B4_3028 0.65 +0.9 3,406,299 + +1.7 3,406,299 + Pf1N1B4_3028 0.65 +0.1 3,406,299 + Pf1N1B4_3028 0.65 -1.9 3,406,299 + Pf1N1B4_3028 0.65 -0.2 3,406,299 + Pf1N1B4_3028 0.65 +1.9 3,406,299 + Pf1N1B4_3028 0.65 -1.5 3,406,300 - +1.1 3,406,300 - Pf1N1B4_3028 0.66 -0.4 3,406,300 - Pf1N1B4_3028 0.66 +0.3 3,406,300 - Pf1N1B4_3028 0.66 +0.2 3,406,300 - Pf1N1B4_3028 0.66 -1.5 3,406,300 - Pf1N1B4_3028 0.66 +0.7 3,406,300 - Pf1N1B4_3028 0.66 -0.5 3,406,358 - Pf1N1B4_3028 0.71 +0.1 3,406,358 - Pf1N1B4_3028 0.71 -0.1
Or see this region's nucleotide sequence