Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_235 and Pf1N1B4_236 are separated by 22 nucleotides Pf1N1B4_236 and Pf1N1B4_237 are separated by 81 nucleotides Pf1N1B4_237 and Pf1N1B4_238 are separated by 76 nucleotides
Pf1N1B4_235 - Molybdopterin biosynthesis protein MoeA, at 295,218 to 296,405
_235
Pf1N1B4_236 - Molybdenum cofactor biosynthesis protein MoaB, at 296,428 to 296,967
_236
Pf1N1B4_237 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 297,049 to 297,651
_237
Pf1N1B4_238 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 297,728 to 297,949
_238
Position (kb) 296
297 Strain fitness (log2 ratio) -2
-1
0
1
2 at 295.483 kb on + strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.777 kb on - strand, within Pf1N1B4_235 at 295.812 kb on - strand, within Pf1N1B4_235 at 295.819 kb on + strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.949 kb on + strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.240 kb on - strand, within Pf1N1B4_235 at 296.638 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.838 kb on + strand, within Pf1N1B4_236 at 296.884 kb on - strand, within Pf1N1B4_236 at 296.947 kb on + strand at 296.948 kb on - strand at 296.948 kb on - strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.968 kb on - strand at 296.968 kb on - strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.984 kb on - strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.004 kb on - strand at 297.004 kb on - strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.006 kb on - strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.046 kb on - strand at 297.046 kb on - strand at 297.060 kb on + strand at 297.060 kb on + strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.238 kb on + strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.342 kb on - strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.609 kb on + strand at 297.623 kb on + strand at 297.677 kb on + strand at 297.677 kb on + strand at 297.678 kb on - strand at 297.678 kb on - strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.680 kb on - strand at 297.800 kb on - strand at 297.947 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 295,483 + Pf1N1B4_235 0.22 +0.5 295,735 - Pf1N1B4_235 0.44 -0.2 295,735 - Pf1N1B4_235 0.44 +0.6 295,735 - Pf1N1B4_235 0.44 -2.5 295,777 - Pf1N1B4_235 0.47 +0.3 295,812 - Pf1N1B4_235 0.50 -0.2 295,819 + Pf1N1B4_235 0.51 -1.5 295,820 - Pf1N1B4_235 0.51 -0.2 295,820 - Pf1N1B4_235 0.51 +0.5 295,820 - Pf1N1B4_235 0.51 +2.1 295,820 - Pf1N1B4_235 0.51 -1.8 295,820 - Pf1N1B4_235 0.51 -0.6 295,949 + Pf1N1B4_235 0.62 +0.3 296,053 - Pf1N1B4_235 0.70 -0.1 296,053 - Pf1N1B4_235 0.70 -1.8 296,148 - Pf1N1B4_235 0.78 +0.2 296,148 - Pf1N1B4_235 0.78 -0.5 296,240 - Pf1N1B4_235 0.86 -0.2 296,638 + Pf1N1B4_236 0.39 -0.2 296,821 + Pf1N1B4_236 0.73 +0.2 296,821 + Pf1N1B4_236 0.73 -0.8 296,821 + Pf1N1B4_236 0.73 -1.2 296,838 + Pf1N1B4_236 0.76 -1.8 296,884 - Pf1N1B4_236 0.84 -1.4 296,947 + +0.6 296,948 - -0.2 296,948 - +1.0 296,967 + +2.6 296,967 + +0.2 296,967 + -0.4 296,968 - -0.9 296,968 - +0.8 296,983 + +0.1 296,983 + -0.5 296,983 + -1.2 296,984 - +1.0 297,003 + +0.0 297,003 + -0.4 297,003 + -0.8 297,003 + -0.9 297,003 + -0.8 297,003 + -2.2 297,003 + +0.8 297,003 + +0.8 297,003 + -0.5 297,004 - -0.2 297,004 - +0.1 297,005 + -0.2 297,005 + +1.6 297,005 + -1.2 297,006 - -0.2 297,045 + +0.4 297,045 + -1.2 297,045 + -0.6 297,045 + -0.2 297,045 + +0.4 297,046 - -1.2 297,046 - +1.2 297,060 + -0.2 297,060 + -1.2 297,061 - -0.6 297,061 - -0.3 297,061 - -1.2 297,238 + Pf1N1B4_237 0.31 +0.8 297,239 - Pf1N1B4_237 0.32 +2.4 297,239 - Pf1N1B4_237 0.32 +0.8 297,239 - Pf1N1B4_237 0.32 -0.2 297,341 + Pf1N1B4_237 0.48 +0.4 297,341 + Pf1N1B4_237 0.48 -2.0 297,341 + Pf1N1B4_237 0.48 +0.5 297,342 - Pf1N1B4_237 0.49 -2.5 297,543 + Pf1N1B4_237 0.82 -2.2 297,543 + Pf1N1B4_237 0.82 +1.1 297,609 + -0.8 297,623 + -0.2 297,677 + -0.4 297,677 + -0.9 297,678 - -0.2 297,678 - -0.8 297,679 + +1.0 297,679 + -0.6 297,679 + -0.2 297,679 + -1.0 297,679 + -1.2 297,679 + +0.8 297,679 + +0.4 297,679 + -1.8 297,680 - -0.2 297,800 - -0.2 297,947 + -0.1
Or see this region's nucleotide sequence