Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1889 and Pf1N1B4_1890 are separated by 119 nucleotides Pf1N1B4_1890 and Pf1N1B4_1891 overlap by 4 nucleotides Pf1N1B4_1891 and Pf1N1B4_1892 are separated by 1 nucleotides Pf1N1B4_1892 and Pf1N1B4_1893 overlap by 4 nucleotides
Pf1N1B4_1889 - Fused spore maturation proteins A and B, at 2,161,622 to 2,162,851
_1889
Pf1N1B4_1890 - Membrane lipoprotein lipid attachment site containing protein USSDB6D, at 2,162,971 to 2,163,597
_1890
Pf1N1B4_1891 - ABC-type transport system involved in resistance to organic solvents, periplasmic component USSDB6C, at 2,163,594 to 2,164,532
_1891
Pf1N1B4_1892 - ABC transporter ATP-binding protein USSDB6B, at 2,164,534 to 2,165,337
_1892
Pf1N1B4_1893 - ABC-type transport system involved in resistance to organic solvents, permease component USSDB6A, at 2,165,334 to 2,166,467
_1893
Position (kb)
2163
2164
2165 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2162.664 kb on + strand, within Pf1N1B4_1889 at 2162.665 kb on - strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.834 kb on + strand at 2162.918 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.954 kb on - strand at 2162.954 kb on - strand at 2162.954 kb on - strand at 2162.972 kb on + strand at 2162.973 kb on - strand at 2162.973 kb on - strand at 2162.973 kb on - strand at 2163.201 kb on - strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.362 kb on + strand, within Pf1N1B4_1890 at 2163.362 kb on + strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.509 kb on + strand, within Pf1N1B4_1890 at 2163.510 kb on - strand, within Pf1N1B4_1890 at 2163.634 kb on + strand at 2163.791 kb on + strand, within Pf1N1B4_1891 at 2163.792 kb on - strand, within Pf1N1B4_1891 at 2164.357 kb on + strand, within Pf1N1B4_1891 at 2164.358 kb on - strand, within Pf1N1B4_1891 at 2164.358 kb on - strand, within Pf1N1B4_1891 at 2164.485 kb on + strand at 2164.486 kb on - strand at 2164.486 kb on - strand at 2164.509 kb on + strand at 2164.509 kb on + strand at 2164.509 kb on + strand at 2164.509 kb on + strand at 2164.510 kb on - strand at 2164.510 kb on - strand at 2164.510 kb on - strand at 2164.510 kb on - strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.596 kb on - strand at 2164.596 kb on - strand at 2164.596 kb on - strand at 2164.697 kb on + strand, within Pf1N1B4_1892 at 2164.799 kb on - strand, within Pf1N1B4_1892 at 2164.901 kb on + strand, within Pf1N1B4_1892 at 2164.902 kb on - strand, within Pf1N1B4_1892 at 2165.231 kb on + strand, within Pf1N1B4_1892 at 2165.231 kb on + strand, within Pf1N1B4_1892 at 2165.231 kb on + strand, within Pf1N1B4_1892 at 2165.231 kb on + strand, within Pf1N1B4_1892 at 2165.231 kb on + strand, within Pf1N1B4_1892 at 2165.232 kb on - strand, within Pf1N1B4_1892 at 2165.232 kb on - strand, within Pf1N1B4_1892 at 2165.232 kb on - strand, within Pf1N1B4_1892 at 2165.232 kb on - strand, within Pf1N1B4_1892 at 2165.232 kb on - strand, within Pf1N1B4_1892 at 2165.232 kb on - strand, within Pf1N1B4_1892
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 2,162,664 + Pf1N1B4_1889 0.85 -0.4 2,162,665 - Pf1N1B4_1889 0.85 +0.3 2,162,723 + Pf1N1B4_1889 0.90 -0.5 2,162,723 + Pf1N1B4_1889 0.90 +0.1 2,162,723 + Pf1N1B4_1889 0.90 -0.7 2,162,723 + Pf1N1B4_1889 0.90 -0.9 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.4 2,162,723 + Pf1N1B4_1889 0.90 -2.2 2,162,723 + Pf1N1B4_1889 0.90 -0.2 2,162,723 + Pf1N1B4_1889 0.90 -0.2 2,162,723 + Pf1N1B4_1889 0.90 +0.2 2,162,723 + Pf1N1B4_1889 0.90 -0.3 2,162,723 + Pf1N1B4_1889 0.90 -0.1 2,162,723 + Pf1N1B4_1889 0.90 -0.7 2,162,723 + Pf1N1B4_1889 0.90 +0.2 2,162,723 + Pf1N1B4_1889 0.90 -0.4 2,162,723 + Pf1N1B4_1889 0.90 -0.4 2,162,723 + Pf1N1B4_1889 0.90 +0.5 2,162,723 + Pf1N1B4_1889 0.90 -0.8 2,162,723 + Pf1N1B4_1889 0.90 +0.5 2,162,723 + Pf1N1B4_1889 0.90 +0.3 2,162,723 + Pf1N1B4_1889 0.90 -1.7 2,162,723 + Pf1N1B4_1889 0.90 +0.7 2,162,723 + Pf1N1B4_1889 0.90 +0.7 2,162,723 + Pf1N1B4_1889 0.90 -0.6 2,162,724 - Pf1N1B4_1889 0.90 -0.3 2,162,724 - Pf1N1B4_1889 0.90 +1.1 2,162,724 - Pf1N1B4_1889 0.90 -1.5 2,162,724 - Pf1N1B4_1889 0.90 +1.8 2,162,724 - Pf1N1B4_1889 0.90 +0.3 2,162,724 - Pf1N1B4_1889 0.90 +0.0 2,162,724 - Pf1N1B4_1889 0.90 +0.8 2,162,724 - Pf1N1B4_1889 0.90 -0.9 2,162,724 - Pf1N1B4_1889 0.90 -0.7 2,162,724 - Pf1N1B4_1889 0.90 -1.7 2,162,724 - Pf1N1B4_1889 0.90 -2.7 2,162,724 - Pf1N1B4_1889 0.90 +0.8 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 +1.3 2,162,724 - Pf1N1B4_1889 0.90 -0.2 2,162,724 - Pf1N1B4_1889 0.90 +0.2 2,162,724 - Pf1N1B4_1889 0.90 -0.1 2,162,724 - Pf1N1B4_1889 0.90 +0.0 2,162,724 - Pf1N1B4_1889 0.90 -1.2 2,162,724 - Pf1N1B4_1889 0.90 +0.3 2,162,724 - Pf1N1B4_1889 0.90 +0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.2 2,162,724 - Pf1N1B4_1889 0.90 -0.1 2,162,724 - Pf1N1B4_1889 0.90 -0.3 2,162,724 - Pf1N1B4_1889 0.90 -0.1 2,162,724 - Pf1N1B4_1889 0.90 +0.3 2,162,724 - Pf1N1B4_1889 0.90 -0.4 2,162,724 - Pf1N1B4_1889 0.90 -0.3 2,162,724 - Pf1N1B4_1889 0.90 +0.3 2,162,727 + Pf1N1B4_1889 0.90 -0.7 2,162,727 + Pf1N1B4_1889 0.90 -0.5 2,162,727 + Pf1N1B4_1889 0.90 +1.5 2,162,727 + Pf1N1B4_1889 0.90 -0.4 2,162,728 - Pf1N1B4_1889 0.90 +0.5 2,162,728 - +0.3 2,162,728 - Pf1N1B4_1889 0.90 +1.2 2,162,728 - Pf1N1B4_1889 0.90 -1.2 2,162,728 - Pf1N1B4_1889 0.90 +0.6 2,162,738 + +0.6 2,162,738 + +1.0 2,162,738 + -1.9 2,162,738 + +0.0 2,162,739 - +0.3 2,162,739 - +0.6 2,162,739 - +0.1 2,162,739 - +0.3 2,162,739 - +0.9 2,162,739 - +0.3 2,162,739 - -1.4 2,162,739 - +1.6 2,162,739 - +0.0 2,162,739 - -1.2 2,162,834 + +0.5 2,162,918 + +0.6 2,162,953 + -1.7 2,162,953 + -0.3 2,162,953 + +0.3 2,162,953 + +1.3 2,162,953 + +0.3 2,162,953 + +1.9 2,162,953 + -0.2 2,162,953 + +0.8 2,162,953 + +0.3 2,162,953 + +0.3 2,162,953 + -0.7 2,162,953 + -0.2 2,162,953 + -0.7 2,162,953 + -0.1 2,162,953 + +1.1 2,162,953 + +0.3 2,162,953 + -1.2 2,162,954 - +1.3 2,162,954 - +0.7 2,162,954 - -1.2 2,162,972 + +0.8 2,162,973 - -0.1 2,162,973 - +0.9 2,162,973 - +0.3 2,163,201 - Pf1N1B4_1890 0.37 +0.5 2,163,255 + Pf1N1B4_1890 0.45 -0.9 2,163,255 + Pf1N1B4_1890 0.45 -1.2 2,163,255 + Pf1N1B4_1890 0.45 -2.0 2,163,256 - Pf1N1B4_1890 0.45 +1.9 2,163,256 - Pf1N1B4_1890 0.45 +0.2 2,163,256 - Pf1N1B4_1890 0.45 -0.4 2,163,362 + Pf1N1B4_1890 0.62 +0.9 2,163,362 + Pf1N1B4_1890 0.62 -2.8 2,163,363 - Pf1N1B4_1890 0.63 +0.3 2,163,363 - Pf1N1B4_1890 0.63 +0.8 2,163,363 - Pf1N1B4_1890 0.63 -0.4 2,163,509 + Pf1N1B4_1890 0.86 +0.9 2,163,510 - Pf1N1B4_1890 0.86 -0.7 2,163,634 + +0.9 2,163,791 + Pf1N1B4_1891 0.21 +0.9 2,163,792 - Pf1N1B4_1891 0.21 -1.4 2,164,357 + Pf1N1B4_1891 0.81 +0.9 2,164,358 - Pf1N1B4_1891 0.81 -3.6 2,164,358 - Pf1N1B4_1891 0.81 -1.2 2,164,485 + +1.7 2,164,486 - -1.2 2,164,486 - -0.2 2,164,509 + +0.3 2,164,509 + -0.1 2,164,509 + -0.5 2,164,509 + -1.2 2,164,510 - +0.2 2,164,510 - -0.7 2,164,510 - -0.2 2,164,510 - -0.1 2,164,595 + -1.7 2,164,595 + -1.0 2,164,595 + -0.4 2,164,595 + -1.1 2,164,595 + -0.4 2,164,595 + +0.3 2,164,595 + -0.4 2,164,596 - +1.3 2,164,596 - +0.3 2,164,596 - +0.1 2,164,697 + Pf1N1B4_1892 0.20 +0.3 2,164,799 - Pf1N1B4_1892 0.33 +1.1 2,164,901 + Pf1N1B4_1892 0.46 -0.1 2,164,902 - Pf1N1B4_1892 0.46 -0.1 2,165,231 + Pf1N1B4_1892 0.87 -1.2 2,165,231 + Pf1N1B4_1892 0.87 -2.2 2,165,231 + Pf1N1B4_1892 0.87 +0.6 2,165,231 + Pf1N1B4_1892 0.87 -0.4 2,165,231 + Pf1N1B4_1892 0.87 +2.2 2,165,232 - Pf1N1B4_1892 0.87 -1.0 2,165,232 - Pf1N1B4_1892 0.87 +0.3 2,165,232 - Pf1N1B4_1892 0.87 +1.3 2,165,232 - Pf1N1B4_1892 0.87 -0.7 2,165,232 - Pf1N1B4_1892 0.87 +1.3 2,165,232 - Pf1N1B4_1892 0.87 -0.5
Or see this region's nucleotide sequence