Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1544 and Pf1N1B4_1545 are separated by 251 nucleotides Pf1N1B4_1545 and Pf1N1B4_1546 are separated by 183 nucleotides Pf1N1B4_1546 and Pf1N1B4_1547 are separated by 13 nucleotides
Pf1N1B4_1544 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 1,779,960 to 1,782,407
_1544
Pf1N1B4_1545 - NADP-dependent malic enzyme (EC 1.1.1.40), at 1,782,659 to 1,783,927
_1545
Pf1N1B4_1546 - Thermonuclease family protein, at 1,784,111 to 1,784,911
_1546
Pf1N1B4_1547 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-dependent, at 1,784,925 to 1,785,155
_1547
Position (kb)
1782
1783
1784 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1781.706 kb on + strand, within Pf1N1B4_1544 at 1781.721 kb on + strand, within Pf1N1B4_1544 at 1781.762 kb on - strand at 1781.762 kb on - strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1782.066 kb on + strand, within Pf1N1B4_1544 at 1782.079 kb on - strand, within Pf1N1B4_1544 at 1782.079 kb on - strand, within Pf1N1B4_1544 at 1782.171 kb on + strand at 1782.171 kb on + strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.291 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.414 kb on - strand at 1782.419 kb on + strand at 1782.419 kb on + strand at 1782.420 kb on - strand at 1782.420 kb on - strand at 1782.423 kb on + strand at 1782.423 kb on + strand at 1782.423 kb on + strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.593 kb on + strand at 1782.594 kb on - strand at 1782.696 kb on + strand at 1782.697 kb on - strand at 1782.697 kb on - strand at 1782.697 kb on - strand at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.837 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1783.227 kb on + strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.546 kb on + strand, within Pf1N1B4_1545 at 1783.547 kb on - strand, within Pf1N1B4_1545 at 1783.547 kb on - strand, within Pf1N1B4_1545 at 1783.555 kb on + strand, within Pf1N1B4_1545 at 1783.555 kb on + strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.651 kb on + strand, within Pf1N1B4_1545 at 1783.651 kb on + strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.661 kb on - strand, within Pf1N1B4_1545 at 1783.661 kb on - strand, within Pf1N1B4_1545 at 1783.752 kb on + strand, within Pf1N1B4_1545 at 1783.752 kb on + strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.758 kb on + strand, within Pf1N1B4_1545 at 1783.758 kb on + strand, within Pf1N1B4_1545 at 1783.791 kb on - strand, within Pf1N1B4_1545 at 1783.813 kb on + strand at 1783.815 kb on + strand at 1783.815 kb on + strand at 1783.815 kb on + strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.945 kb on + strand at 1783.946 kb on - strand at 1783.946 kb on - strand at 1783.973 kb on + strand at 1783.973 kb on + strand at 1783.974 kb on - strand at 1784.294 kb on + strand, within Pf1N1B4_1546 at 1784.294 kb on + strand, within Pf1N1B4_1546 at 1784.295 kb on - strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.719 kb on + strand, within Pf1N1B4_1546
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 1,781,706 + Pf1N1B4_1544 0.71 -2.3 1,781,721 + Pf1N1B4_1544 0.72 -1.7 1,781,762 - -0.1 1,781,762 - Pf1N1B4_1544 0.74 -1.7 1,781,934 + Pf1N1B4_1544 0.81 -0.1 1,781,934 + Pf1N1B4_1544 0.81 -2.5 1,781,934 + Pf1N1B4_1544 0.81 -2.0 1,781,934 + Pf1N1B4_1544 0.81 -1.1 1,781,934 + Pf1N1B4_1544 0.81 -1.3 1,781,934 + Pf1N1B4_1544 0.81 -1.7 1,781,934 + Pf1N1B4_1544 0.81 +0.3 1,781,934 + Pf1N1B4_1544 0.81 +1.9 1,781,934 + Pf1N1B4_1544 0.81 -0.7 1,781,935 - Pf1N1B4_1544 0.81 -0.7 1,781,935 - Pf1N1B4_1544 0.81 +0.3 1,781,935 - Pf1N1B4_1544 0.81 -0.7 1,781,935 - -0.7 1,781,935 - Pf1N1B4_1544 0.81 -2.5 1,781,935 - Pf1N1B4_1544 0.81 -0.7 1,781,935 - Pf1N1B4_1544 0.81 -2.9 1,781,935 - Pf1N1B4_1544 0.81 -0.1 1,781,935 - Pf1N1B4_1544 0.81 -0.1 1,781,935 - Pf1N1B4_1544 0.81 -1.3 1,781,935 - Pf1N1B4_1544 0.81 -1.1 1,782,066 + Pf1N1B4_1544 0.86 -0.7 1,782,079 - Pf1N1B4_1544 0.87 +0.3 1,782,079 - Pf1N1B4_1544 0.87 -0.3 1,782,171 + +0.3 1,782,171 + +0.3 1,782,172 - -0.7 1,782,172 - +0.3 1,782,172 - +0.3 1,782,172 - +0.3 1,782,291 + +0.3 1,782,413 + +0.5 1,782,413 + +1.5 1,782,413 + -0.1 1,782,413 + +0.3 1,782,413 + -0.1 1,782,414 - -0.9 1,782,419 + +0.6 1,782,419 + -2.3 1,782,420 - +1.5 1,782,420 - -0.4 1,782,423 + +0.3 1,782,423 + -0.1 1,782,423 + -0.3 1,782,424 - +0.3 1,782,424 - -0.7 1,782,424 - +0.6 1,782,424 - +0.6 1,782,593 + -1.3 1,782,594 - +1.3 1,782,696 + -1.7 1,782,697 - -0.8 1,782,697 - -0.4 1,782,697 - -2.1 1,782,804 + Pf1N1B4_1545 0.11 +0.3 1,782,804 + Pf1N1B4_1545 0.11 -1.0 1,782,804 + Pf1N1B4_1545 0.11 -1.3 1,782,804 + Pf1N1B4_1545 0.11 -0.7 1,782,804 + Pf1N1B4_1545 0.11 -1.3 1,782,804 + Pf1N1B4_1545 0.11 -1.1 1,782,804 + Pf1N1B4_1545 0.11 +0.3 1,782,805 - Pf1N1B4_1545 0.12 -1.3 1,782,805 - Pf1N1B4_1545 0.12 -1.3 1,782,805 - Pf1N1B4_1545 0.12 -2.1 1,782,805 - Pf1N1B4_1545 0.12 -1.6 1,782,805 - Pf1N1B4_1545 0.12 -2.1 1,782,805 - Pf1N1B4_1545 0.12 -0.7 1,782,805 - Pf1N1B4_1545 0.12 -0.3 1,782,805 - Pf1N1B4_1545 0.12 -0.3 1,782,837 + Pf1N1B4_1545 0.14 +0.3 1,782,974 + Pf1N1B4_1545 0.25 +1.3 1,782,974 + Pf1N1B4_1545 0.25 -0.7 1,782,974 + Pf1N1B4_1545 0.25 +0.3 1,782,975 - Pf1N1B4_1545 0.25 -0.3 1,782,975 - Pf1N1B4_1545 0.25 +1.7 1,782,975 - Pf1N1B4_1545 0.25 -2.6 1,783,227 + Pf1N1B4_1545 0.45 +0.3 1,783,228 - Pf1N1B4_1545 0.45 -0.3 1,783,228 - Pf1N1B4_1545 0.45 +1.9 1,783,228 - Pf1N1B4_1545 0.45 +0.3 1,783,281 + Pf1N1B4_1545 0.49 -1.7 1,783,281 + Pf1N1B4_1545 0.49 +0.5 1,783,281 + Pf1N1B4_1545 0.49 -2.1 1,783,281 + Pf1N1B4_1545 0.49 -2.5 1,783,281 + Pf1N1B4_1545 0.49 -1.1 1,783,281 + -0.7 1,783,281 + Pf1N1B4_1545 0.49 +0.3 1,783,281 + Pf1N1B4_1545 0.49 -1.7 1,783,282 - Pf1N1B4_1545 0.49 -2.7 1,783,282 - Pf1N1B4_1545 0.49 -0.1 1,783,282 - Pf1N1B4_1545 0.49 -1.7 1,783,282 - Pf1N1B4_1545 0.49 -2.7 1,783,282 - Pf1N1B4_1545 0.49 -1.3 1,783,282 - Pf1N1B4_1545 0.49 -1.3 1,783,282 - Pf1N1B4_1545 0.49 -2.7 1,783,282 - Pf1N1B4_1545 0.49 +0.3 1,783,282 - Pf1N1B4_1545 0.49 -2.1 1,783,282 - Pf1N1B4_1545 0.49 +0.3 1,783,282 - Pf1N1B4_1545 0.49 +0.3 1,783,282 - Pf1N1B4_1545 0.49 -1.1 1,783,282 - Pf1N1B4_1545 0.49 -1.1 1,783,282 - Pf1N1B4_1545 0.49 -0.1 1,783,282 - Pf1N1B4_1545 0.49 -1.7 1,783,282 - Pf1N1B4_1545 0.49 +0.3 1,783,546 + Pf1N1B4_1545 0.70 -0.7 1,783,547 - Pf1N1B4_1545 0.70 -0.3 1,783,547 - Pf1N1B4_1545 0.70 +0.3 1,783,555 + Pf1N1B4_1545 0.71 -1.1 1,783,555 + Pf1N1B4_1545 0.71 -2.3 1,783,556 - Pf1N1B4_1545 0.71 -2.4 1,783,556 - Pf1N1B4_1545 0.71 +0.5 1,783,556 - Pf1N1B4_1545 0.71 -1.0 1,783,556 - Pf1N1B4_1545 0.71 -0.7 1,783,651 + Pf1N1B4_1545 0.78 -0.7 1,783,651 + Pf1N1B4_1545 0.78 -2.7 1,783,652 - Pf1N1B4_1545 0.78 -1.7 1,783,652 - Pf1N1B4_1545 0.78 -0.5 1,783,652 - Pf1N1B4_1545 0.78 +0.3 1,783,661 - Pf1N1B4_1545 0.79 -1.1 1,783,661 - Pf1N1B4_1545 0.79 -0.1 1,783,752 + Pf1N1B4_1545 0.86 +0.3 1,783,752 + Pf1N1B4_1545 0.86 -1.7 1,783,753 - Pf1N1B4_1545 0.86 -0.1 1,783,753 - Pf1N1B4_1545 0.86 -0.5 1,783,753 - Pf1N1B4_1545 0.86 -1.3 1,783,753 - Pf1N1B4_1545 0.86 -0.3 1,783,753 - Pf1N1B4_1545 0.86 +2.4 1,783,753 - Pf1N1B4_1545 0.86 -0.3 1,783,753 - Pf1N1B4_1545 0.86 +0.3 1,783,753 - Pf1N1B4_1545 0.86 -2.3 1,783,753 - Pf1N1B4_1545 0.86 -2.3 1,783,758 + Pf1N1B4_1545 0.87 -0.5 1,783,758 + Pf1N1B4_1545 0.87 -2.1 1,783,791 - Pf1N1B4_1545 0.89 -0.3 1,783,813 + -1.2 1,783,815 + -2.1 1,783,815 + -0.1 1,783,815 + -2.9 1,783,816 - -1.1 1,783,816 - -1.3 1,783,816 - -1.3 1,783,816 - +0.3 1,783,927 + -0.7 1,783,927 + +0.3 1,783,927 + -0.3 1,783,927 + -2.1 1,783,928 - -1.7 1,783,928 - -0.1 1,783,928 - -1.3 1,783,928 - -0.3 1,783,945 + +0.3 1,783,946 - +0.3 1,783,946 - +1.9 1,783,973 + -3.1 1,783,973 + -2.4 1,783,974 - -0.1 1,784,294 + Pf1N1B4_1546 0.23 +0.3 1,784,294 + Pf1N1B4_1546 0.23 -0.7 1,784,295 - Pf1N1B4_1546 0.23 -0.5 1,784,562 + Pf1N1B4_1546 0.56 +0.9 1,784,562 + Pf1N1B4_1546 0.56 +0.3 1,784,562 + Pf1N1B4_1546 0.56 +0.3 1,784,562 + Pf1N1B4_1546 0.56 -0.4 1,784,562 + Pf1N1B4_1546 0.56 -0.7 1,784,562 + Pf1N1B4_1546 0.56 +1.3 1,784,562 + Pf1N1B4_1546 0.56 +0.3 1,784,563 - Pf1N1B4_1546 0.56 +0.3 1,784,563 - Pf1N1B4_1546 0.56 -1.1 1,784,563 - Pf1N1B4_1546 0.56 -0.1 1,784,563 - Pf1N1B4_1546 0.56 -0.1 1,784,563 - Pf1N1B4_1546 0.56 -1.7 1,784,563 - Pf1N1B4_1546 0.56 -1.3 1,784,719 + Pf1N1B4_1546 0.76 -1.0
Or see this region's nucleotide sequence