Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1515 and Pf1N1B4_1516 overlap by 1 nucleotides Pf1N1B4_1516 and Pf1N1B4_1517 overlap by 4 nucleotides Pf1N1B4_1517 and Pf1N1B4_1518 overlap by 4 nucleotides Pf1N1B4_1518 and Pf1N1B4_1519 overlap by 1 nucleotides
Pf1N1B4_1515 - FIG025233: SAM-dependent methyltransferases, at 1,748,778 to 1,749,503
_1515
Pf1N1B4_1516 - FIG085779: Lipoprotein, at 1,749,503 to 1,749,985
_1516
Pf1N1B4_1517 - 3-oxoacyl-[ACP] synthase (EC 2.3.1.41) FabV like, at 1,749,982 to 1,751,178
_1517
Pf1N1B4_1518 - 3-hydroxydecanoyl-[ACP] dehydratase (EC 4.2.1.60), at 1,751,175 to 1,751,633
_1518
Pf1N1B4_1519 - 3-oxoacyl-[ACP] reductase (EC 1.1.1.100), at 1,751,633 to 1,752,361
_1519
Position (kb)
1749
1750
1751
1752 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1749.067 kb on + strand, within Pf1N1B4_1515 at 1749.067 kb on + strand, within Pf1N1B4_1515 at 1749.067 kb on + strand, within Pf1N1B4_1515 at 1749.068 kb on - strand, within Pf1N1B4_1515 at 1749.068 kb on - strand, within Pf1N1B4_1515 at 1749.068 kb on - strand, within Pf1N1B4_1515 at 1749.068 kb on - strand, within Pf1N1B4_1515 at 1749.068 kb on - strand, within Pf1N1B4_1515 at 1749.187 kb on - strand, within Pf1N1B4_1515 at 1749.211 kb on - strand, within Pf1N1B4_1515 at 1749.240 kb on + strand, within Pf1N1B4_1515 at 1749.240 kb on + strand, within Pf1N1B4_1515 at 1749.240 kb on + strand, within Pf1N1B4_1515 at 1749.240 kb on + strand, within Pf1N1B4_1515 at 1749.240 kb on + strand, within Pf1N1B4_1515 at 1749.240 kb on + strand, within Pf1N1B4_1515 at 1749.240 kb on + strand, within Pf1N1B4_1515 at 1749.241 kb on - strand, within Pf1N1B4_1515 at 1749.241 kb on - strand, within Pf1N1B4_1515 at 1749.241 kb on - strand, within Pf1N1B4_1515 at 1749.241 kb on - strand, within Pf1N1B4_1515 at 1749.248 kb on + strand, within Pf1N1B4_1515 at 1749.248 kb on + strand, within Pf1N1B4_1515 at 1749.248 kb on + strand, within Pf1N1B4_1515 at 1749.248 kb on + strand, within Pf1N1B4_1515 at 1749.249 kb on - strand, within Pf1N1B4_1515 at 1749.363 kb on + strand, within Pf1N1B4_1515 at 1749.363 kb on + strand, within Pf1N1B4_1515 at 1749.364 kb on - strand, within Pf1N1B4_1515 at 1749.369 kb on + strand, within Pf1N1B4_1515 at 1749.369 kb on + strand, within Pf1N1B4_1515 at 1749.369 kb on + strand, within Pf1N1B4_1515 at 1749.370 kb on - strand, within Pf1N1B4_1515 at 1749.370 kb on - strand, within Pf1N1B4_1515 at 1749.370 kb on - strand at 1749.370 kb on - strand, within Pf1N1B4_1515 at 1749.373 kb on + strand, within Pf1N1B4_1515 at 1749.373 kb on + strand, within Pf1N1B4_1515 at 1749.373 kb on + strand, within Pf1N1B4_1515 at 1749.373 kb on + strand, within Pf1N1B4_1515 at 1749.373 kb on + strand, within Pf1N1B4_1515 at 1749.373 kb on + strand, within Pf1N1B4_1515 at 1749.373 kb on + strand, within Pf1N1B4_1515 at 1749.373 kb on + strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.374 kb on - strand, within Pf1N1B4_1515 at 1749.469 kb on + strand at 1749.469 kb on + strand at 1749.470 kb on - strand at 1749.495 kb on + strand at 1749.496 kb on - strand at 1749.496 kb on - strand at 1749.842 kb on + strand, within Pf1N1B4_1516 at 1749.912 kb on + strand, within Pf1N1B4_1516 at 1749.912 kb on + strand, within Pf1N1B4_1516 at 1749.913 kb on - strand, within Pf1N1B4_1516 at 1749.913 kb on - strand, within Pf1N1B4_1516 at 1749.991 kb on + strand at 1749.992 kb on - strand at 1749.992 kb on - strand at 1749.992 kb on - strand at 1749.992 kb on - strand at 1750.263 kb on + strand, within Pf1N1B4_1517 at 1750.327 kb on + strand, within Pf1N1B4_1517 at 1750.327 kb on + strand, within Pf1N1B4_1517 at 1750.328 kb on - strand at 1750.358 kb on - strand, within Pf1N1B4_1517 at 1750.364 kb on - strand, within Pf1N1B4_1517 at 1750.432 kb on + strand, within Pf1N1B4_1517 at 1750.433 kb on - strand, within Pf1N1B4_1517 at 1750.433 kb on - strand, within Pf1N1B4_1517 at 1750.732 kb on - strand, within Pf1N1B4_1517 at 1750.732 kb on - strand, within Pf1N1B4_1517 at 1750.889 kb on + strand, within Pf1N1B4_1517 at 1750.890 kb on - strand, within Pf1N1B4_1517 at 1750.890 kb on - strand, within Pf1N1B4_1517 at 1750.890 kb on - strand, within Pf1N1B4_1517 at 1750.939 kb on - strand, within Pf1N1B4_1517 at 1751.491 kb on + strand, within Pf1N1B4_1518 at 1751.492 kb on - strand, within Pf1N1B4_1518 at 1751.492 kb on - strand, within Pf1N1B4_1518 at 1751.605 kb on - strand at 1751.840 kb on - strand, within Pf1N1B4_1519 at 1751.840 kb on - strand, within Pf1N1B4_1519 at 1751.873 kb on + strand, within Pf1N1B4_1519 at 1751.874 kb on - strand, within Pf1N1B4_1519 at 1751.876 kb on + strand, within Pf1N1B4_1519 at 1751.876 kb on + strand, within Pf1N1B4_1519 at 1751.877 kb on - strand, within Pf1N1B4_1519 at 1751.978 kb on + strand, within Pf1N1B4_1519 at 1751.979 kb on - strand, within Pf1N1B4_1519 at 1751.979 kb on - strand, within Pf1N1B4_1519 at 1752.089 kb on + strand, within Pf1N1B4_1519 at 1752.090 kb on - strand, within Pf1N1B4_1519
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 1,749,067 + Pf1N1B4_1515 0.40 -0.7 1,749,067 + Pf1N1B4_1515 0.40 -2.1 1,749,067 + Pf1N1B4_1515 0.40 +0.7 1,749,068 - Pf1N1B4_1515 0.40 +0.3 1,749,068 - Pf1N1B4_1515 0.40 -0.3 1,749,068 - Pf1N1B4_1515 0.40 -0.6 1,749,068 - Pf1N1B4_1515 0.40 +0.3 1,749,068 - Pf1N1B4_1515 0.40 +0.3 1,749,187 - Pf1N1B4_1515 0.56 -0.0 1,749,211 - Pf1N1B4_1515 0.60 +0.3 1,749,240 + Pf1N1B4_1515 0.64 -1.2 1,749,240 + Pf1N1B4_1515 0.64 +0.3 1,749,240 + Pf1N1B4_1515 0.64 -0.3 1,749,240 + Pf1N1B4_1515 0.64 +1.7 1,749,240 + Pf1N1B4_1515 0.64 -1.5 1,749,240 + Pf1N1B4_1515 0.64 +1.8 1,749,240 + Pf1N1B4_1515 0.64 +0.3 1,749,241 - Pf1N1B4_1515 0.64 +0.3 1,749,241 - Pf1N1B4_1515 0.64 -1.3 1,749,241 - Pf1N1B4_1515 0.64 +0.9 1,749,241 - Pf1N1B4_1515 0.64 +2.6 1,749,248 + Pf1N1B4_1515 0.65 +0.8 1,749,248 + Pf1N1B4_1515 0.65 +0.9 1,749,248 + Pf1N1B4_1515 0.65 +0.3 1,749,248 + Pf1N1B4_1515 0.65 +0.9 1,749,249 - Pf1N1B4_1515 0.65 +0.7 1,749,363 + Pf1N1B4_1515 0.81 -1.1 1,749,363 + Pf1N1B4_1515 0.81 +0.1 1,749,364 - Pf1N1B4_1515 0.81 -0.5 1,749,369 + Pf1N1B4_1515 0.81 -0.7 1,749,369 + Pf1N1B4_1515 0.81 -0.2 1,749,369 + Pf1N1B4_1515 0.81 -0.7 1,749,370 - Pf1N1B4_1515 0.82 -0.2 1,749,370 - Pf1N1B4_1515 0.82 -1.5 1,749,370 - +0.3 1,749,370 - Pf1N1B4_1515 0.82 -0.1 1,749,373 + Pf1N1B4_1515 0.82 -1.3 1,749,373 + Pf1N1B4_1515 0.82 -1.3 1,749,373 + Pf1N1B4_1515 0.82 -1.3 1,749,373 + Pf1N1B4_1515 0.82 +0.3 1,749,373 + Pf1N1B4_1515 0.82 -1.2 1,749,373 + Pf1N1B4_1515 0.82 -0.2 1,749,373 + Pf1N1B4_1515 0.82 -0.4 1,749,373 + Pf1N1B4_1515 0.82 +0.3 1,749,374 - Pf1N1B4_1515 0.82 +0.7 1,749,374 - Pf1N1B4_1515 0.82 +0.3 1,749,374 - Pf1N1B4_1515 0.82 -1.3 1,749,374 - Pf1N1B4_1515 0.82 +0.3 1,749,374 - Pf1N1B4_1515 0.82 +0.3 1,749,374 - Pf1N1B4_1515 0.82 +0.7 1,749,374 - Pf1N1B4_1515 0.82 -1.4 1,749,374 - Pf1N1B4_1515 0.82 -1.3 1,749,374 - Pf1N1B4_1515 0.82 -1.3 1,749,374 - Pf1N1B4_1515 0.82 +0.9 1,749,469 + +0.8 1,749,469 + +0.3 1,749,470 - -0.5 1,749,495 + +0.8 1,749,496 - +0.3 1,749,496 - -0.7 1,749,842 + Pf1N1B4_1516 0.70 -0.3 1,749,912 + Pf1N1B4_1516 0.85 -0.7 1,749,912 + Pf1N1B4_1516 0.85 -1.7 1,749,913 - Pf1N1B4_1516 0.85 -0.7 1,749,913 - Pf1N1B4_1516 0.85 -1.0 1,749,991 + -0.7 1,749,992 - +2.4 1,749,992 - +0.7 1,749,992 - +0.6 1,749,992 - -0.2 1,750,263 + Pf1N1B4_1517 0.23 -0.3 1,750,327 + Pf1N1B4_1517 0.29 -1.7 1,750,327 + Pf1N1B4_1517 0.29 +0.3 1,750,328 - -0.7 1,750,358 - Pf1N1B4_1517 0.31 -1.1 1,750,364 - Pf1N1B4_1517 0.32 -0.3 1,750,432 + Pf1N1B4_1517 0.38 -0.5 1,750,433 - Pf1N1B4_1517 0.38 -0.2 1,750,433 - Pf1N1B4_1517 0.38 -1.3 1,750,732 - Pf1N1B4_1517 0.63 +0.3 1,750,732 - Pf1N1B4_1517 0.63 -0.7 1,750,889 + Pf1N1B4_1517 0.76 -0.2 1,750,890 - Pf1N1B4_1517 0.76 +0.3 1,750,890 - Pf1N1B4_1517 0.76 +0.3 1,750,890 - Pf1N1B4_1517 0.76 -1.6 1,750,939 - Pf1N1B4_1517 0.80 +0.8 1,751,491 + Pf1N1B4_1518 0.69 +0.7 1,751,492 - Pf1N1B4_1518 0.69 +1.3 1,751,492 - Pf1N1B4_1518 0.69 -1.7 1,751,605 - +0.3 1,751,840 - Pf1N1B4_1519 0.28 -1.7 1,751,840 - Pf1N1B4_1519 0.28 +0.3 1,751,873 + Pf1N1B4_1519 0.33 +1.0 1,751,874 - Pf1N1B4_1519 0.33 +1.7 1,751,876 + Pf1N1B4_1519 0.33 -1.3 1,751,876 + Pf1N1B4_1519 0.33 +0.5 1,751,877 - Pf1N1B4_1519 0.33 +0.6 1,751,978 + Pf1N1B4_1519 0.47 +1.8 1,751,979 - Pf1N1B4_1519 0.47 -0.3 1,751,979 - Pf1N1B4_1519 0.47 +0.6 1,752,089 + Pf1N1B4_1519 0.63 -2.1 1,752,090 - Pf1N1B4_1519 0.63 +0.3
Or see this region's nucleotide sequence