Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1188 and Pf1N1B4_1189 overlap by 1 nucleotides Pf1N1B4_1189 and Pf1N1B4_1190 overlap by 4 nucleotides Pf1N1B4_1190 and Pf1N1B4_1191 are separated by 129 nucleotides Pf1N1B4_1191 and Pf1N1B4_1192 are separated by 398 nucleotides
Pf1N1B4_1188 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 1,339,178 to 1,340,632
_1188
Pf1N1B4_1189 - Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG, at 1,340,632 to 1,341,303
_1189
Pf1N1B4_1190 - Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing, at 1,341,300 to 1,344,110
_1190
Pf1N1B4_1191 - hypothetical protein, at 1,344,240 to 1,344,521
_1191
Pf1N1B4_1192 - hypothetical protein, at 1,344,920 to 1,345,105
_1192
Position (kb)
1341
1342
1343
1344
1345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1340.417 kb on + strand, within Pf1N1B4_1188 at 1340.417 kb on + strand, within Pf1N1B4_1188 at 1340.417 kb on + strand, within Pf1N1B4_1188 at 1340.418 kb on - strand, within Pf1N1B4_1188 at 1340.418 kb on - strand, within Pf1N1B4_1188 at 1340.438 kb on - strand, within Pf1N1B4_1188 at 1340.649 kb on + strand at 1340.649 kb on + strand at 1340.650 kb on - strand at 1340.650 kb on - strand at 1340.650 kb on - strand at 1340.731 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.953 kb on + strand, within Pf1N1B4_1189 at 1340.953 kb on + strand at 1340.953 kb on + strand, within Pf1N1B4_1189 at 1340.954 kb on - strand, within Pf1N1B4_1189 at 1341.046 kb on - strand, within Pf1N1B4_1189 at 1341.242 kb on + strand at 1341.242 kb on + strand at 1341.242 kb on + strand at 1341.242 kb on + strand at 1341.774 kb on + strand, within Pf1N1B4_1190 at 1341.774 kb on + strand, within Pf1N1B4_1190 at 1341.774 kb on + strand, within Pf1N1B4_1190 at 1341.774 kb on + strand, within Pf1N1B4_1190 at 1341.774 kb on + strand, within Pf1N1B4_1190 at 1341.775 kb on - strand, within Pf1N1B4_1190 at 1341.775 kb on - strand, within Pf1N1B4_1190 at 1341.775 kb on - strand, within Pf1N1B4_1190 at 1341.951 kb on + strand, within Pf1N1B4_1190 at 1341.952 kb on - strand, within Pf1N1B4_1190 at 1341.952 kb on - strand, within Pf1N1B4_1190 at 1341.952 kb on - strand, within Pf1N1B4_1190 at 1341.952 kb on - strand, within Pf1N1B4_1190 at 1341.952 kb on - strand, within Pf1N1B4_1190 at 1341.966 kb on - strand, within Pf1N1B4_1190 at 1342.017 kb on - strand, within Pf1N1B4_1190 at 1342.062 kb on - strand, within Pf1N1B4_1190 at 1342.077 kb on + strand, within Pf1N1B4_1190 at 1342.077 kb on + strand, within Pf1N1B4_1190 at 1342.078 kb on - strand, within Pf1N1B4_1190 at 1342.078 kb on - strand, within Pf1N1B4_1190 at 1342.078 kb on - strand, within Pf1N1B4_1190 at 1342.078 kb on - strand, within Pf1N1B4_1190 at 1342.135 kb on + strand at 1342.135 kb on + strand, within Pf1N1B4_1190 at 1342.136 kb on - strand, within Pf1N1B4_1190 at 1342.136 kb on - strand, within Pf1N1B4_1190 at 1342.136 kb on - strand, within Pf1N1B4_1190 at 1342.136 kb on - strand, within Pf1N1B4_1190 at 1342.252 kb on + strand, within Pf1N1B4_1190 at 1342.253 kb on - strand, within Pf1N1B4_1190 at 1342.779 kb on + strand, within Pf1N1B4_1190 at 1342.817 kb on + strand, within Pf1N1B4_1190 at 1342.914 kb on - strand, within Pf1N1B4_1190 at 1342.914 kb on - strand, within Pf1N1B4_1190 at 1343.004 kb on - strand, within Pf1N1B4_1190 at 1343.055 kb on + strand, within Pf1N1B4_1190 at 1343.075 kb on + strand, within Pf1N1B4_1190 at 1343.076 kb on - strand, within Pf1N1B4_1190 at 1343.116 kb on + strand, within Pf1N1B4_1190 at 1343.231 kb on + strand, within Pf1N1B4_1190 at 1343.232 kb on - strand, within Pf1N1B4_1190 at 1343.412 kb on + strand, within Pf1N1B4_1190 at 1343.412 kb on + strand, within Pf1N1B4_1190 at 1343.412 kb on + strand, within Pf1N1B4_1190 at 1343.412 kb on + strand, within Pf1N1B4_1190 at 1343.413 kb on - strand, within Pf1N1B4_1190 at 1343.413 kb on - strand, within Pf1N1B4_1190 at 1343.459 kb on + strand, within Pf1N1B4_1190 at 1343.460 kb on - strand, within Pf1N1B4_1190 at 1343.661 kb on + strand, within Pf1N1B4_1190 at 1343.661 kb on + strand, within Pf1N1B4_1190 at 1343.661 kb on + strand, within Pf1N1B4_1190 at 1343.661 kb on + strand, within Pf1N1B4_1190 at 1343.661 kb on + strand, within Pf1N1B4_1190 at 1343.662 kb on - strand, within Pf1N1B4_1190 at 1343.662 kb on - strand, within Pf1N1B4_1190 at 1343.662 kb on - strand, within Pf1N1B4_1190 at 1343.727 kb on + strand, within Pf1N1B4_1190 at 1343.727 kb on + strand, within Pf1N1B4_1190 at 1343.727 kb on + strand, within Pf1N1B4_1190 at 1343.727 kb on + strand, within Pf1N1B4_1190 at 1343.727 kb on + strand, within Pf1N1B4_1190 at 1343.728 kb on - strand, within Pf1N1B4_1190 at 1343.728 kb on - strand, within Pf1N1B4_1190 at 1343.728 kb on - strand, within Pf1N1B4_1190 at 1343.728 kb on - strand, within Pf1N1B4_1190 at 1343.873 kb on + strand at 1343.874 kb on - strand at 1344.058 kb on - strand at 1344.072 kb on + strand at 1344.072 kb on + strand at 1344.072 kb on + strand at 1344.073 kb on - strand at 1344.073 kb on - strand at 1344.073 kb on - strand at 1344.118 kb on + strand at 1344.119 kb on - strand at 1344.254 kb on - strand at 1344.259 kb on + strand at 1344.259 kb on + strand at 1344.260 kb on - strand at 1344.420 kb on + strand, within Pf1N1B4_1191 at 1344.420 kb on + strand, within Pf1N1B4_1191 at 1344.421 kb on - strand, within Pf1N1B4_1191 at 1344.421 kb on - strand, within Pf1N1B4_1191 at 1344.490 kb on + strand at 1344.490 kb on + strand, within Pf1N1B4_1191 at 1344.491 kb on - strand, within Pf1N1B4_1191 at 1344.513 kb on - strand at 1344.523 kb on - strand at 1344.565 kb on + strand at 1344.565 kb on + strand at 1344.566 kb on - strand at 1344.566 kb on - strand at 1344.566 kb on - strand at 1344.572 kb on + strand at 1344.573 kb on - strand at 1344.645 kb on + strand at 1344.646 kb on - strand at 1344.664 kb on + strand at 1344.665 kb on - strand at 1344.665 kb on - strand at 1344.826 kb on - strand at 1344.890 kb on + strand at 1344.891 kb on - strand at 1344.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 1,340,417 + Pf1N1B4_1188 0.85 -2.2 1,340,417 + Pf1N1B4_1188 0.85 +2.7 1,340,417 + Pf1N1B4_1188 0.85 -0.5 1,340,418 - Pf1N1B4_1188 0.85 +1.5 1,340,418 - Pf1N1B4_1188 0.85 -0.1 1,340,438 - Pf1N1B4_1188 0.87 -0.5 1,340,649 + +0.1 1,340,649 + +0.7 1,340,650 - -0.2 1,340,650 - -1.9 1,340,650 - -1.6 1,340,731 + Pf1N1B4_1189 0.15 +0.1 1,340,737 + Pf1N1B4_1189 0.16 -0.8 1,340,737 + Pf1N1B4_1189 0.16 +0.9 1,340,737 + Pf1N1B4_1189 0.16 +0.1 1,340,737 + Pf1N1B4_1189 0.16 +1.7 1,340,737 + Pf1N1B4_1189 0.16 +0.1 1,340,738 - Pf1N1B4_1189 0.16 -0.7 1,340,738 - Pf1N1B4_1189 0.16 -1.5 1,340,738 - Pf1N1B4_1189 0.16 -1.2 1,340,738 - Pf1N1B4_1189 0.16 +0.1 1,340,738 - Pf1N1B4_1189 0.16 -0.9 1,340,738 - Pf1N1B4_1189 0.16 +0.9 1,340,738 - Pf1N1B4_1189 0.16 -1.5 1,340,953 + Pf1N1B4_1189 0.48 -0.9 1,340,953 + +0.1 1,340,953 + Pf1N1B4_1189 0.48 -2.2 1,340,954 - Pf1N1B4_1189 0.48 -0.2 1,341,046 - Pf1N1B4_1189 0.62 +0.1 1,341,242 + -0.5 1,341,242 + +1.7 1,341,242 + -1.3 1,341,242 + -0.5 1,341,774 + Pf1N1B4_1190 0.17 +0.1 1,341,774 + Pf1N1B4_1190 0.17 -1.9 1,341,774 + Pf1N1B4_1190 0.17 -0.3 1,341,774 + Pf1N1B4_1190 0.17 +2.9 1,341,774 + Pf1N1B4_1190 0.17 -0.7 1,341,775 - Pf1N1B4_1190 0.17 +0.1 1,341,775 - Pf1N1B4_1190 0.17 +1.3 1,341,775 - Pf1N1B4_1190 0.17 +0.1 1,341,951 + Pf1N1B4_1190 0.23 -1.9 1,341,952 - Pf1N1B4_1190 0.23 -1.1 1,341,952 - Pf1N1B4_1190 0.23 +0.8 1,341,952 - Pf1N1B4_1190 0.23 -1.2 1,341,952 - Pf1N1B4_1190 0.23 +0.1 1,341,952 - Pf1N1B4_1190 0.23 +0.1 1,341,966 - Pf1N1B4_1190 0.24 +0.1 1,342,017 - Pf1N1B4_1190 0.26 -2.2 1,342,062 - Pf1N1B4_1190 0.27 +1.1 1,342,077 + Pf1N1B4_1190 0.28 -0.9 1,342,077 + Pf1N1B4_1190 0.28 +0.1 1,342,078 - Pf1N1B4_1190 0.28 +0.7 1,342,078 - Pf1N1B4_1190 0.28 -1.9 1,342,078 - Pf1N1B4_1190 0.28 -0.9 1,342,078 - Pf1N1B4_1190 0.28 +0.1 1,342,135 + +1.1 1,342,135 + Pf1N1B4_1190 0.30 -0.5 1,342,136 - Pf1N1B4_1190 0.30 +0.1 1,342,136 - Pf1N1B4_1190 0.30 -0.3 1,342,136 - Pf1N1B4_1190 0.30 -0.3 1,342,136 - Pf1N1B4_1190 0.30 -0.5 1,342,252 + Pf1N1B4_1190 0.34 -1.5 1,342,253 - Pf1N1B4_1190 0.34 -0.6 1,342,779 + Pf1N1B4_1190 0.53 -0.9 1,342,817 + Pf1N1B4_1190 0.54 -0.6 1,342,914 - Pf1N1B4_1190 0.57 -0.1 1,342,914 - Pf1N1B4_1190 0.57 -2.7 1,343,004 - Pf1N1B4_1190 0.61 +0.6 1,343,055 + Pf1N1B4_1190 0.62 -0.9 1,343,075 + Pf1N1B4_1190 0.63 -0.2 1,343,076 - Pf1N1B4_1190 0.63 -2.2 1,343,116 + Pf1N1B4_1190 0.65 -1.2 1,343,231 + Pf1N1B4_1190 0.69 -2.9 1,343,232 - Pf1N1B4_1190 0.69 -0.1 1,343,412 + Pf1N1B4_1190 0.75 -0.6 1,343,412 + Pf1N1B4_1190 0.75 +0.1 1,343,412 + Pf1N1B4_1190 0.75 -0.7 1,343,412 + Pf1N1B4_1190 0.75 -0.9 1,343,413 - Pf1N1B4_1190 0.75 -0.7 1,343,413 - Pf1N1B4_1190 0.75 -0.6 1,343,459 + Pf1N1B4_1190 0.77 -0.9 1,343,460 - Pf1N1B4_1190 0.77 -0.9 1,343,661 + Pf1N1B4_1190 0.84 -0.9 1,343,661 + Pf1N1B4_1190 0.84 -1.3 1,343,661 + Pf1N1B4_1190 0.84 -0.9 1,343,661 + Pf1N1B4_1190 0.84 +0.3 1,343,661 + Pf1N1B4_1190 0.84 +0.1 1,343,662 - Pf1N1B4_1190 0.84 -0.7 1,343,662 - Pf1N1B4_1190 0.84 +0.1 1,343,662 - Pf1N1B4_1190 0.84 -0.9 1,343,727 + Pf1N1B4_1190 0.86 -0.9 1,343,727 + Pf1N1B4_1190 0.86 -0.1 1,343,727 + Pf1N1B4_1190 0.86 -1.1 1,343,727 + Pf1N1B4_1190 0.86 -1.2 1,343,727 + Pf1N1B4_1190 0.86 -0.3 1,343,728 - Pf1N1B4_1190 0.86 -1.1 1,343,728 - Pf1N1B4_1190 0.86 -1.3 1,343,728 - Pf1N1B4_1190 0.86 +0.1 1,343,728 - Pf1N1B4_1190 0.86 +0.6 1,343,873 + -0.3 1,343,874 - -0.5 1,344,058 - +1.5 1,344,072 + -2.5 1,344,072 + -2.7 1,344,072 + -0.0 1,344,073 - -0.5 1,344,073 - -0.5 1,344,073 - +0.9 1,344,118 + +0.0 1,344,119 - +1.3 1,344,254 - +0.1 1,344,259 + +1.4 1,344,259 + +1.1 1,344,260 - +0.1 1,344,420 + Pf1N1B4_1191 0.64 +1.1 1,344,420 + Pf1N1B4_1191 0.64 -0.6 1,344,421 - Pf1N1B4_1191 0.64 -1.7 1,344,421 - Pf1N1B4_1191 0.64 +1.1 1,344,490 + +0.1 1,344,490 + Pf1N1B4_1191 0.89 -0.0 1,344,491 - Pf1N1B4_1191 0.89 +2.4 1,344,513 - -1.5 1,344,523 - +0.9 1,344,565 + -0.1 1,344,565 + -0.0 1,344,566 - +0.7 1,344,566 - -2.5 1,344,566 - -0.0 1,344,572 + -0.9 1,344,573 - -1.6 1,344,645 + +0.4 1,344,646 - +1.1 1,344,664 + +0.4 1,344,665 - -0.3 1,344,665 - -0.9 1,344,826 - -1.1 1,344,890 + +1.1 1,344,891 - +1.1 1,344,919 - -1.9
Or see this region's nucleotide sequence