Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_732

Experiment: N1B4wt with N2E2m denitrifying replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_730 and Pf6N2E2_731 are separated by 2 nucleotidesPf6N2E2_731 and Pf6N2E2_732 overlap by 8 nucleotidesPf6N2E2_732 and Pf6N2E2_733 are separated by 10 nucleotides Pf6N2E2_730 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 838,011 to 839,549 _730 Pf6N2E2_731 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 839,552 to 840,307 _731 Pf6N2E2_732 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 840,300 to 840,980 _732 Pf6N2E2_733 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 840,991 to 841,959 _733 Position (kb) 840 841Strain fitness (log2 ratio) -3 -2 -1 0 1at 839.337 kb on + strand, within Pf6N2E2_730at 839.338 kb on - strand, within Pf6N2E2_730at 839.338 kb on - strand, within Pf6N2E2_730at 839.338 kb on - strand, within Pf6N2E2_730at 839.361 kb on + strand, within Pf6N2E2_730at 839.361 kb on + strand, within Pf6N2E2_730at 839.361 kb on + strand, within Pf6N2E2_730at 839.362 kb on - strand, within Pf6N2E2_730at 839.362 kb on - strand, within Pf6N2E2_730at 839.362 kb on - strand, within Pf6N2E2_730at 839.380 kb on + strand, within Pf6N2E2_730at 839.880 kb on + strand, within Pf6N2E2_731at 839.880 kb on + strand, within Pf6N2E2_731at 839.881 kb on - strand, within Pf6N2E2_731at 839.881 kb on - strand, within Pf6N2E2_731at 839.881 kb on - strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 840.298 kb on + strandat 840.298 kb on + strandat 840.305 kb on + strandat 840.305 kb on + strandat 840.306 kb on - strandat 840.306 kb on - strandat 840.396 kb on + strand, within Pf6N2E2_732at 840.397 kb on - strand, within Pf6N2E2_732at 840.397 kb on - strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.740 kb on + strand, within Pf6N2E2_732at 840.852 kb on + strand, within Pf6N2E2_732at 840.852 kb on + strand, within Pf6N2E2_732at 840.853 kb on - strand, within Pf6N2E2_732at 840.940 kb on - strandat 840.940 kb on - strandat 841.246 kb on + strand, within Pf6N2E2_733at 841.247 kb on - strand, within Pf6N2E2_733at 841.454 kb on - strand, within Pf6N2E2_733at 841.454 kb on - strand, within Pf6N2E2_733at 841.454 kb on - strand, within Pf6N2E2_733at 841.882 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate B
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839,337 + Pf6N2E2_730 0.86 +1.0
839,338 - Pf6N2E2_730 0.86 -0.7
839,338 - Pf6N2E2_730 0.86 -0.5
839,338 - Pf6N2E2_730 0.86 -1.9
839,361 + Pf6N2E2_730 0.88 -1.6
839,361 + Pf6N2E2_730 0.88 -1.2
839,361 + Pf6N2E2_730 0.88 -0.3
839,362 - Pf6N2E2_730 0.88 -3.5
839,362 - Pf6N2E2_730 0.88 -0.9
839,362 - Pf6N2E2_730 0.88 -1.2
839,380 + Pf6N2E2_730 0.89 -1.6
839,880 + Pf6N2E2_731 0.43 +0.4
839,880 + Pf6N2E2_731 0.43 -1.8
839,881 - Pf6N2E2_731 0.44 -0.9
839,881 - Pf6N2E2_731 0.44 -2.0
839,881 - Pf6N2E2_731 0.44 -2.4
839,888 + Pf6N2E2_731 0.44 +1.6
839,888 + Pf6N2E2_731 0.44 -2.3
839,888 + Pf6N2E2_731 0.44 -2.4
839,888 + Pf6N2E2_731 0.44 -3.1
839,888 + Pf6N2E2_731 0.44 -2.0
839,888 + Pf6N2E2_731 0.44 -3.3
839,888 + Pf6N2E2_731 0.44 -0.8
840,298 + -0.7
840,298 + -0.9
840,305 + -3.4
840,305 + +0.4
840,306 - -1.9
840,306 - -2.1
840,396 + Pf6N2E2_732 0.14 -1.7
840,397 - Pf6N2E2_732 0.14 -1.6
840,397 - Pf6N2E2_732 0.14 -1.3
840,528 + Pf6N2E2_732 0.33 -1.3
840,528 + Pf6N2E2_732 0.33 -3.0
840,528 + Pf6N2E2_732 0.33 -0.1
840,528 + Pf6N2E2_732 0.33 -1.0
840,528 + Pf6N2E2_732 0.33 -1.4
840,528 + Pf6N2E2_732 0.33 -1.0
840,528 + Pf6N2E2_732 0.33 -2.0
840,528 + Pf6N2E2_732 0.33 -2.3
840,529 - Pf6N2E2_732 0.34 -1.6
840,529 - Pf6N2E2_732 0.34 -2.1
840,529 - Pf6N2E2_732 0.34 -2.6
840,529 - Pf6N2E2_732 0.34 -1.6
840,529 - Pf6N2E2_732 0.34 -0.7
840,529 - Pf6N2E2_732 0.34 -1.6
840,740 + Pf6N2E2_732 0.65 -0.9
840,852 + Pf6N2E2_732 0.81 -0.6
840,852 + Pf6N2E2_732 0.81 +0.2
840,853 - Pf6N2E2_732 0.81 -1.8
840,940 - -0.2
840,940 - -0.6
841,246 + Pf6N2E2_733 0.26 +1.1
841,247 - Pf6N2E2_733 0.26 -1.7
841,454 - Pf6N2E2_733 0.48 -0.8
841,454 - Pf6N2E2_733 0.48 +0.2
841,454 - Pf6N2E2_733 0.48 -0.7
841,882 + -0.2

Or see this region's nucleotide sequence