Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_702

Experiment: N1B4wt with N2E2m denitrifying replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotidesPf6N2E2_700 and Pf6N2E2_701 overlap by 4 nucleotidesPf6N2E2_701 and Pf6N2E2_702 are separated by 2 nucleotidesPf6N2E2_702 and Pf6N2E2_703 overlap by 4 nucleotidesPf6N2E2_703 and Pf6N2E2_704 are separated by 190 nucleotidesPf6N2E2_704 and Pf6N2E2_705 are separated by 24 nucleotides Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052 _699 Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557 _700 Pf6N2E2_701 - Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL, at 806,554 to 807,012 _701 Pf6N2E2_702 - Heme d1 biosynthesis protein NirF, at 807,015 to 808,193 _702 Pf6N2E2_703 - Cytochrome c55X precursor NirC, at 808,190 to 808,519 _703 Pf6N2E2_704 - Cytochrome c551/c552, at 808,710 to 809,018 _704 Pf6N2E2_705 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 809,043 to 810,722 _705 Position (kb) 807 808 809Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.224 kb on - strand, within Pf6N2E2_700at 806.257 kb on - strand, within Pf6N2E2_700at 806.708 kb on + strand, within Pf6N2E2_701at 806.708 kb on + strand, within Pf6N2E2_701at 806.709 kb on - strand, within Pf6N2E2_701at 806.726 kb on + strand, within Pf6N2E2_701at 806.726 kb on + strand, within Pf6N2E2_701at 806.727 kb on - strand, within Pf6N2E2_701at 806.727 kb on - strand, within Pf6N2E2_701at 806.727 kb on - strand, within Pf6N2E2_701at 806.727 kb on - strand, within Pf6N2E2_701at 807.451 kb on + strand, within Pf6N2E2_702at 807.452 kb on - strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.572 kb on - strand, within Pf6N2E2_702at 807.572 kb on - strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.602 kb on - strand, within Pf6N2E2_702at 807.602 kb on - strand, within Pf6N2E2_702at 807.814 kb on - strand, within Pf6N2E2_702at 807.835 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.971 kb on - strand, within Pf6N2E2_702at 807.971 kb on - strand, within Pf6N2E2_702at 807.971 kb on - strand, within Pf6N2E2_702at 807.971 kb on - strand, within Pf6N2E2_702at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.378 kb on - strand, within Pf6N2E2_703at 808.378 kb on - strand, within Pf6N2E2_703at 808.534 kb on + strandat 808.534 kb on + strandat 808.535 kb on - strandat 808.711 kb on + strandat 808.712 kb on - strandat 808.712 kb on - strandat 808.712 kb on - strandat 808.715 kb on + strandat 808.716 kb on - strandat 808.716 kb on - strandat 808.805 kb on + strand, within Pf6N2E2_704at 808.806 kb on - strand, within Pf6N2E2_704at 808.806 kb on - strand, within Pf6N2E2_704at 808.901 kb on + strand, within Pf6N2E2_704at 808.902 kb on - strand, within Pf6N2E2_704at 809.010 kb on - strandat 809.047 kb on + strandat 809.047 kb on + strandat 809.047 kb on + strandat 809.047 kb on + strandat 809.047 kb on + strandat 809.048 kb on - strandat 809.048 kb on - strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.069 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate B
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806,223 + Pf6N2E2_700 0.35 -1.2
806,223 + Pf6N2E2_700 0.35 -0.4
806,223 + Pf6N2E2_700 0.35 -1.0
806,223 + Pf6N2E2_700 0.35 -0.6
806,223 + Pf6N2E2_700 0.35 +0.5
806,223 + Pf6N2E2_700 0.35 -0.9
806,224 - Pf6N2E2_700 0.35 -2.3
806,257 - Pf6N2E2_700 0.41 -0.7
806,708 + Pf6N2E2_701 0.34 -2.3
806,708 + Pf6N2E2_701 0.34 -0.5
806,709 - Pf6N2E2_701 0.34 -0.6
806,726 + Pf6N2E2_701 0.37 -1.5
806,726 + Pf6N2E2_701 0.37 -1.2
806,727 - Pf6N2E2_701 0.38 -1.2
806,727 - Pf6N2E2_701 0.38 -1.3
806,727 - Pf6N2E2_701 0.38 -0.6
806,727 - Pf6N2E2_701 0.38 -1.3
807,451 + Pf6N2E2_702 0.37 -1.3
807,452 - Pf6N2E2_702 0.37 -1.3
807,568 + Pf6N2E2_702 0.47 -1.1
807,568 + Pf6N2E2_702 0.47 -1.0
807,568 + Pf6N2E2_702 0.47 -0.9
807,568 + Pf6N2E2_702 0.47 -1.8
807,568 + Pf6N2E2_702 0.47 -1.8
807,568 + Pf6N2E2_702 0.47 +0.0
807,568 + Pf6N2E2_702 0.47 -1.0
807,568 + Pf6N2E2_702 0.47 -1.3
807,568 + Pf6N2E2_702 0.47 +0.1
807,568 + Pf6N2E2_702 0.47 -2.0
807,569 - Pf6N2E2_702 0.47 -1.0
807,569 - Pf6N2E2_702 0.47 -1.2
807,569 - Pf6N2E2_702 0.47 -2.3
807,569 - Pf6N2E2_702 0.47 -0.9
807,569 - Pf6N2E2_702 0.47 -1.2
807,572 - Pf6N2E2_702 0.47 +1.0
807,572 - Pf6N2E2_702 0.47 -0.7
807,601 + Pf6N2E2_702 0.50 -0.6
807,601 + Pf6N2E2_702 0.50 +1.0
807,601 + Pf6N2E2_702 0.50 -1.3
807,601 + Pf6N2E2_702 0.50 -0.7
807,601 + Pf6N2E2_702 0.50 +1.0
807,602 - Pf6N2E2_702 0.50 -0.8
807,602 - Pf6N2E2_702 0.50 -2.5
807,814 - Pf6N2E2_702 0.68 -0.3
807,835 + Pf6N2E2_702 0.70 -0.6
807,970 + Pf6N2E2_702 0.81 -0.6
807,970 + Pf6N2E2_702 0.81 -0.2
807,970 + Pf6N2E2_702 0.81 -0.8
807,970 + Pf6N2E2_702 0.81 -1.2
807,970 + Pf6N2E2_702 0.81 -0.8
807,971 - Pf6N2E2_702 0.81 -1.0
807,971 - Pf6N2E2_702 0.81 +0.4
807,971 - Pf6N2E2_702 0.81 -1.2
807,971 - Pf6N2E2_702 0.81 -0.6
808,377 + Pf6N2E2_703 0.57 -1.0
808,377 + Pf6N2E2_703 0.57 -0.9
808,377 + Pf6N2E2_703 0.57 -0.8
808,377 + Pf6N2E2_703 0.57 -0.7
808,377 + Pf6N2E2_703 0.57 -0.8
808,377 + Pf6N2E2_703 0.57 +2.0
808,378 - Pf6N2E2_703 0.57 -3.4
808,378 - Pf6N2E2_703 0.57 -0.4
808,534 + -1.8
808,534 + -0.4
808,535 - +1.0
808,711 + -1.0
808,712 - -0.7
808,712 - +0.2
808,712 - -1.0
808,715 + -0.6
808,716 - -0.5
808,716 - +0.0
808,805 + Pf6N2E2_704 0.31 -1.3
808,806 - Pf6N2E2_704 0.31 -0.3
808,806 - Pf6N2E2_704 0.31 -0.9
808,901 + Pf6N2E2_704 0.62 -1.0
808,902 - Pf6N2E2_704 0.62 -0.7
809,010 - +0.0
809,047 + -0.6
809,047 + -1.6
809,047 + -1.1
809,047 + +0.5
809,047 + -1.4
809,048 - +0.1
809,048 - -0.5
809,068 + -0.2
809,068 + -0.3
809,068 + -1.2
809,068 + -1.2
809,068 + +1.0
809,068 + -0.7
809,068 + -0.3
809,069 - -0.4

Or see this region's nucleotide sequence