Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_64

Experiment: N1B4wt with N2E2m denitrifying replicate B

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	N1B4wt with N2E2m denitrifying replicate B
103,748	+		Pf6N2E2_63	0.52	-2.4
103,749	-		Pf6N2E2_63	0.52	-3.7
103,749	-		Pf6N2E2_63	0.52	-0.4
103,997	+		Pf6N2E2_63	0.70	-2.0
103,998	-		Pf6N2E2_63	0.70	-3.0
104,318	-				-2.3
104,350	+				-1.3
104,602	+		Pf6N2E2_64	0.25	-3.0
104,603	-		Pf6N2E2_64	0.25	-4.1
104,660	-		Pf6N2E2_64	0.34	-3.5
104,774	-		Pf6N2E2_64	0.52	-2.5
105,092	+				+0.2
105,251	+				-0.3
105,252	-				-1.0
105,252	-				+0.7
105,283	+				+1.0
105,329	+		Pf6N2E2_65	0.14	-0.6
105,330	-		Pf6N2E2_65	0.14	+0.5
105,330	-		Pf6N2E2_65	0.14	+0.2
105,330	-		Pf6N2E2_65	0.14	+1.3
105,330	-		Pf6N2E2_65	0.14	+1.2
105,330	-		Pf6N2E2_65	0.14	-1.3
105,436	+		Pf6N2E2_65	0.28	-0.0
105,436	+		Pf6N2E2_65	0.28	-0.7
105,437	-		Pf6N2E2_65	0.28	+0.6
105,697	+		Pf6N2E2_65	0.62	+1.4
105,698	-		Pf6N2E2_65	0.62	+0.8
105,698	-		Pf6N2E2_65	0.62	-1.1
105,801	+		Pf6N2E2_65	0.76	+0.2
105,802	-		Pf6N2E2_65	0.76	+0.7
105,802	-		Pf6N2E2_65	0.76	+0.8
105,829	+		Pf6N2E2_65	0.79	-0.1
105,829	+		Pf6N2E2_65	0.79	-0.0
105,855	+		Pf6N2E2_65	0.83	-0.2
105,954	+				+1.1
105,955	-				-1.3
106,013	-				+0.2
106,013	-				-1.1