Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5328

Experiment: N1B4wt with N2E2m denitrifying replicate B

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	N1B4wt with N2E2m denitrifying replicate B
6,122,416	+		Pf6N2E2_5327	0.10	+0.3
6,122,417	-		Pf6N2E2_5327	0.10	+0.1
6,122,473	+		Pf6N2E2_5327	0.18	+0.3
6,122,473	+		Pf6N2E2_5327	0.18	-0.4
6,122,473	+		Pf6N2E2_5327	0.18	+0.6
6,122,473	+		Pf6N2E2_5327	0.18	-0.3
6,122,473	+		Pf6N2E2_5327	0.18	+0.3
6,122,594	-		Pf6N2E2_5327	0.34	+1.0
6,122,596	+		Pf6N2E2_5327	0.35	+0.3
6,122,597	-		Pf6N2E2_5327	0.35	+0.4
6,122,597	-		Pf6N2E2_5327	0.35	-0.2
6,122,597	-		Pf6N2E2_5327	0.35	+1.0
6,122,809	+		Pf6N2E2_5327	0.63	-0.1
6,122,881	+		Pf6N2E2_5327	0.73	+1.2
6,122,881	+		Pf6N2E2_5327	0.73	-0.3
6,122,881	+		Pf6N2E2_5327	0.73	-0.2
6,122,882	-		Pf6N2E2_5327	0.73	-0.0
6,122,882	-		Pf6N2E2_5327	0.73	-0.4
6,122,882	-		Pf6N2E2_5327	0.73	+0.4
6,123,028	+				-0.5
6,123,028	+				+0.3
6,123,028	+				+2.1
6,123,061	+				-0.4
6,123,061	+				-1.2
6,123,061	+				+0.3
6,123,061	+				+1.5
6,123,062	-				+0.4
6,123,062	-				-0.6
6,123,062	-				-0.0
6,123,070	+				-0.1
6,123,071	-				+1.0
6,123,090	+				+0.7
6,123,183	+				+0.5
6,123,184	-				+0.1
6,123,317	-				-0.1
6,123,372	+				-0.1
6,123,372	+				-2.0
6,123,372	+				-0.7
6,123,372	+				+0.8
6,123,373	-				+0.1
6,123,373	-				-0.4
6,123,373	-				-0.1
6,123,373	-				+0.4
6,123,433	-		Pf6N2E2_5328	0.14	-1.8
6,123,433	-		Pf6N2E2_5328	0.14	-1.9
6,123,433	-		Pf6N2E2_5328	0.14	-1.2
6,123,588	+		Pf6N2E2_5328	0.50	-0.6
6,123,589	-		Pf6N2E2_5328	0.50	-0.3
6,123,654	+		Pf6N2E2_5328	0.65	-0.6
6,123,654	+		Pf6N2E2_5328	0.65	-2.0
6,123,654	+		Pf6N2E2_5328	0.65	-1.5
6,123,788	+				-0.4
6,123,788	+				-1.9
6,123,788	+				+1.0
6,123,789	-				+0.3
6,123,789	-				-0.7
6,123,818	+				-0.5
6,123,819	-				+0.2
6,123,820	+				+0.3
6,123,835	+				-1.0
6,123,835	+				+0.1
6,123,836	-				-1.0
6,123,836	-				+2.0
6,123,836	-				+0.7
6,123,861	+				-1.4
6,123,861	+				-1.3
6,123,861	+				-1.4
6,123,861	+				-1.1
6,123,861	+				-1.6
6,123,861	+				+1.4
6,123,861	+				+0.6
6,123,862	-				-1.1
6,123,862	-				+0.1
6,123,862	-				-0.2
6,123,862	-				-0.4
6,123,864	-				-0.8
6,123,902	+				-1.4
6,123,903	-				+0.2
6,123,903	-				+0.4
6,123,903	-				-1.6
6,123,906	+				-0.8
6,123,906	+				+0.3
6,123,907	-				+0.4
6,123,907	-				-0.3
6,123,922	-				+0.2
6,123,922	-				+0.3
6,123,922	-				-1.0
6,123,952	+				+0.5
6,123,952	+				-1.0
6,123,953	-				+0.3
6,123,953	-				-0.5
6,123,953	-				+1.4
6,123,953	-				+1.0
6,123,953	-				+1.1
6,123,969	+				-0.0
6,123,970	-				-0.1
6,123,970	-				-1.4
6,124,007	+				-0.5
6,124,007	+				+0.6
6,124,007	+				-1.0
6,124,008	-				+0.5
6,124,008	-				+0.4
6,124,133	-		Pf6N2E2_5329	0.29	-1.1
6,124,148	+		Pf6N2E2_5329	0.32	+1.0
6,124,149	-		Pf6N2E2_5329	0.32	+0.7
6,124,149	-		Pf6N2E2_5329	0.32	+0.2
6,124,158	+		Pf6N2E2_5329	0.33	+0.3
6,124,158	+		Pf6N2E2_5329	0.33	+1.0
6,124,159	-		Pf6N2E2_5329	0.33	-0.6
6,124,460	+		Pf6N2E2_5329	0.81	-0.5
6,124,461	-		Pf6N2E2_5329	0.81	-1.0
6,124,461	-		Pf6N2E2_5329	0.81	-0.0