Experiment: N1B4wt with N2E2m denitrifying replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5237 and Pf6N2E2_5238 are separated by 3 nucleotides Pf6N2E2_5238 and Pf6N2E2_5239 are separated by 126 nucleotides Pf6N2E2_5239 and Pf6N2E2_5240 are separated by 266 nucleotides Pf6N2E2_5240 and Pf6N2E2_5241 are separated by 61 nucleotides
Pf6N2E2_5237 - Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8), at 6,034,169 to 6,034,726
_5237
Pf6N2E2_5238 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 6,034,730 to 6,035,203
_5238
Pf6N2E2_5239 - Putative sulfate permease, at 6,035,330 to 6,037,072
_5239
Pf6N2E2_5240 - FIG00953775: hypothetical protein, at 6,037,339 to 6,037,824
_5240
Pf6N2E2_5241 - Aminopeptidase N, at 6,037,886 to 6,038,173
_5241
Position (kb)
6035
6036
6037
6038 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6034.383 kb on - strand, within Pf6N2E2_5237 at 6034.650 kb on - strand, within Pf6N2E2_5237 at 6034.650 kb on - strand, within Pf6N2E2_5237 at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.674 kb on - strand at 6034.674 kb on - strand at 6034.674 kb on - strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.725 kb on - strand at 6034.725 kb on - strand at 6034.816 kb on + strand, within Pf6N2E2_5238 at 6034.816 kb on + strand, within Pf6N2E2_5238 at 6034.817 kb on - strand, within Pf6N2E2_5238 at 6034.817 kb on - strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6035.042 kb on + strand, within Pf6N2E2_5238 at 6035.267 kb on + strand at 6035.412 kb on + strand at 6035.412 kb on + strand at 6035.412 kb on + strand at 6035.413 kb on - strand at 6035.413 kb on - strand at 6035.413 kb on - strand at 6035.413 kb on - strand at 6035.413 kb on - strand at 6035.413 kb on - strand at 6035.855 kb on + strand, within Pf6N2E2_5239 at 6035.856 kb on - strand, within Pf6N2E2_5239 at 6035.856 kb on - strand, within Pf6N2E2_5239 at 6035.856 kb on - strand, within Pf6N2E2_5239 at 6035.856 kb on - strand, within Pf6N2E2_5239 at 6035.856 kb on - strand, within Pf6N2E2_5239 at 6035.856 kb on - strand, within Pf6N2E2_5239 at 6035.884 kb on + strand, within Pf6N2E2_5239 at 6035.884 kb on + strand, within Pf6N2E2_5239 at 6035.884 kb on + strand, within Pf6N2E2_5239 at 6035.884 kb on + strand, within Pf6N2E2_5239 at 6035.884 kb on + strand, within Pf6N2E2_5239 at 6035.884 kb on + strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.885 kb on - strand, within Pf6N2E2_5239 at 6035.961 kb on + strand, within Pf6N2E2_5239 at 6036.264 kb on + strand, within Pf6N2E2_5239 at 6036.264 kb on + strand, within Pf6N2E2_5239 at 6036.265 kb on - strand, within Pf6N2E2_5239 at 6036.265 kb on - strand, within Pf6N2E2_5239 at 6036.265 kb on - strand, within Pf6N2E2_5239 at 6036.265 kb on - strand, within Pf6N2E2_5239 at 6036.369 kb on + strand, within Pf6N2E2_5239 at 6036.369 kb on + strand, within Pf6N2E2_5239 at 6036.369 kb on + strand, within Pf6N2E2_5239 at 6036.369 kb on + strand, within Pf6N2E2_5239 at 6036.370 kb on - strand, within Pf6N2E2_5239 at 6036.370 kb on - strand, within Pf6N2E2_5239 at 6036.370 kb on - strand, within Pf6N2E2_5239 at 6036.370 kb on - strand, within Pf6N2E2_5239 at 6036.370 kb on - strand, within Pf6N2E2_5239 at 6036.370 kb on - strand, within Pf6N2E2_5239 at 6036.585 kb on + strand, within Pf6N2E2_5239 at 6036.585 kb on + strand, within Pf6N2E2_5239 at 6036.585 kb on + strand, within Pf6N2E2_5239 at 6036.585 kb on + strand, within Pf6N2E2_5239 at 6036.585 kb on + strand, within Pf6N2E2_5239 at 6036.585 kb on + strand, within Pf6N2E2_5239 at 6036.585 kb on + strand, within Pf6N2E2_5239 at 6036.585 kb on + strand, within Pf6N2E2_5239 at 6036.586 kb on - strand, within Pf6N2E2_5239 at 6036.586 kb on - strand, within Pf6N2E2_5239 at 6036.586 kb on - strand, within Pf6N2E2_5239 at 6036.586 kb on - strand, within Pf6N2E2_5239 at 6036.586 kb on - strand, within Pf6N2E2_5239 at 6036.696 kb on + strand, within Pf6N2E2_5239 at 6036.696 kb on + strand, within Pf6N2E2_5239 at 6036.696 kb on + strand, within Pf6N2E2_5239 at 6036.696 kb on + strand, within Pf6N2E2_5239 at 6036.696 kb on + strand, within Pf6N2E2_5239 at 6036.697 kb on - strand, within Pf6N2E2_5239 at 6036.697 kb on - strand, within Pf6N2E2_5239 at 6036.697 kb on - strand, within Pf6N2E2_5239 at 6036.697 kb on - strand, within Pf6N2E2_5239 at 6036.697 kb on - strand, within Pf6N2E2_5239 at 6036.697 kb on - strand, within Pf6N2E2_5239 at 6036.697 kb on - strand, within Pf6N2E2_5239 at 6036.697 kb on - strand, within Pf6N2E2_5239 at 6036.897 kb on + strand, within Pf6N2E2_5239 at 6036.897 kb on + strand, within Pf6N2E2_5239 at 6036.897 kb on + strand, within Pf6N2E2_5239 at 6036.897 kb on + strand, within Pf6N2E2_5239 at 6036.898 kb on - strand at 6036.898 kb on - strand at 6036.898 kb on - strand at 6036.898 kb on - strand at 6037.014 kb on + strand at 6037.076 kb on + strand at 6037.076 kb on + strand at 6037.076 kb on + strand at 6037.076 kb on + strand at 6037.076 kb on + strand at 6037.077 kb on - strand at 6037.077 kb on - strand at 6037.077 kb on - strand at 6037.077 kb on - strand at 6037.143 kb on - strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.154 kb on + strand at 6037.155 kb on - strand at 6037.155 kb on - strand at 6037.155 kb on - strand at 6037.155 kb on - strand at 6037.158 kb on - strand at 6037.158 kb on - strand at 6037.267 kb on + strand at 6037.290 kb on - strand at 6037.307 kb on + strand at 6037.308 kb on - strand at 6037.365 kb on + strand at 6037.498 kb on + strand, within Pf6N2E2_5240 at 6037.623 kb on + strand, within Pf6N2E2_5240 at 6037.624 kb on - strand, within Pf6N2E2_5240 at 6037.696 kb on + strand, within Pf6N2E2_5240 at 6037.697 kb on - strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.750 kb on + strand, within Pf6N2E2_5240 at 6037.751 kb on - strand, within Pf6N2E2_5240 at 6037.751 kb on - strand, within Pf6N2E2_5240 at 6037.768 kb on + strand, within Pf6N2E2_5240 at 6037.769 kb on - strand, within Pf6N2E2_5240 at 6037.769 kb on - strand, within Pf6N2E2_5240 at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.783 kb on - strand at 6037.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate B remove 6,034,383 - Pf6N2E2_5237 0.38 -0.3 6,034,650 - Pf6N2E2_5237 0.86 -0.5 6,034,650 - Pf6N2E2_5237 0.86 +0.1 6,034,673 + -0.4 6,034,673 + +1.6 6,034,673 + +0.4 6,034,673 + -0.3 6,034,674 - +0.3 6,034,674 - +0.4 6,034,674 - +0.6 6,034,724 + +0.6 6,034,724 + +1.0 6,034,724 + +0.0 6,034,724 + -0.5 6,034,725 - +0.3 6,034,725 - +0.0 6,034,816 + Pf6N2E2_5238 0.18 +2.6 6,034,816 + Pf6N2E2_5238 0.18 +1.0 6,034,817 - Pf6N2E2_5238 0.18 +0.2 6,034,817 - Pf6N2E2_5238 0.18 +0.5 6,034,877 + Pf6N2E2_5238 0.31 -0.5 6,034,877 + Pf6N2E2_5238 0.31 -0.0 6,034,877 + Pf6N2E2_5238 0.31 +0.3 6,034,877 + Pf6N2E2_5238 0.31 +0.1 6,034,878 - Pf6N2E2_5238 0.31 +1.0 6,034,878 - Pf6N2E2_5238 0.31 -1.0 6,034,878 - Pf6N2E2_5238 0.31 +0.9 6,034,878 - Pf6N2E2_5238 0.31 +1.2 6,035,042 + Pf6N2E2_5238 0.66 +1.0 6,035,267 + -0.2 6,035,412 + -1.2 6,035,412 + -2.8 6,035,412 + -1.5 6,035,413 - -0.8 6,035,413 - +0.4 6,035,413 - -0.5 6,035,413 - -0.7 6,035,413 - -0.5 6,035,413 - +0.0 6,035,855 + Pf6N2E2_5239 0.30 +0.7 6,035,856 - Pf6N2E2_5239 0.30 -0.4 6,035,856 - Pf6N2E2_5239 0.30 -0.7 6,035,856 - Pf6N2E2_5239 0.30 -1.4 6,035,856 - Pf6N2E2_5239 0.30 -2.0 6,035,856 - Pf6N2E2_5239 0.30 -0.8 6,035,856 - Pf6N2E2_5239 0.30 -0.2 6,035,884 + Pf6N2E2_5239 0.32 -1.3 6,035,884 + Pf6N2E2_5239 0.32 -0.1 6,035,884 + Pf6N2E2_5239 0.32 -0.3 6,035,884 + Pf6N2E2_5239 0.32 -1.1 6,035,884 + Pf6N2E2_5239 0.32 -0.9 6,035,884 + Pf6N2E2_5239 0.32 -0.4 6,035,885 - Pf6N2E2_5239 0.32 -0.6 6,035,885 - Pf6N2E2_5239 0.32 -0.7 6,035,885 - Pf6N2E2_5239 0.32 -0.5 6,035,885 - Pf6N2E2_5239 0.32 -1.3 6,035,885 - Pf6N2E2_5239 0.32 -1.8 6,035,885 - Pf6N2E2_5239 0.32 +0.3 6,035,885 - Pf6N2E2_5239 0.32 +0.0 6,035,885 - Pf6N2E2_5239 0.32 -1.4 6,035,885 - Pf6N2E2_5239 0.32 -1.9 6,035,961 + Pf6N2E2_5239 0.36 -0.1 6,036,264 + Pf6N2E2_5239 0.54 -1.7 6,036,264 + Pf6N2E2_5239 0.54 -0.3 6,036,265 - Pf6N2E2_5239 0.54 -1.4 6,036,265 - Pf6N2E2_5239 0.54 -1.2 6,036,265 - Pf6N2E2_5239 0.54 -0.4 6,036,265 - Pf6N2E2_5239 0.54 -0.2 6,036,369 + Pf6N2E2_5239 0.60 +1.0 6,036,369 + Pf6N2E2_5239 0.60 -2.3 6,036,369 + Pf6N2E2_5239 0.60 -2.0 6,036,369 + Pf6N2E2_5239 0.60 -0.6 6,036,370 - Pf6N2E2_5239 0.60 -1.8 6,036,370 - Pf6N2E2_5239 0.60 +1.4 6,036,370 - Pf6N2E2_5239 0.60 -1.6 6,036,370 - Pf6N2E2_5239 0.60 -1.2 6,036,370 - Pf6N2E2_5239 0.60 -1.1 6,036,370 - Pf6N2E2_5239 0.60 -1.7 6,036,585 + Pf6N2E2_5239 0.72 +0.0 6,036,585 + Pf6N2E2_5239 0.72 -1.7 6,036,585 + Pf6N2E2_5239 0.72 -3.8 6,036,585 + Pf6N2E2_5239 0.72 -0.8 6,036,585 + Pf6N2E2_5239 0.72 -0.7 6,036,585 + Pf6N2E2_5239 0.72 -0.4 6,036,585 + Pf6N2E2_5239 0.72 -0.7 6,036,585 + Pf6N2E2_5239 0.72 -2.7 6,036,586 - Pf6N2E2_5239 0.72 -0.8 6,036,586 - Pf6N2E2_5239 0.72 -1.2 6,036,586 - Pf6N2E2_5239 0.72 +0.6 6,036,586 - Pf6N2E2_5239 0.72 -0.6 6,036,586 - Pf6N2E2_5239 0.72 -1.9 6,036,696 + Pf6N2E2_5239 0.78 -1.2 6,036,696 + Pf6N2E2_5239 0.78 -0.6 6,036,696 + Pf6N2E2_5239 0.78 -0.7 6,036,696 + Pf6N2E2_5239 0.78 -0.7 6,036,696 + Pf6N2E2_5239 0.78 -0.6 6,036,697 - Pf6N2E2_5239 0.78 -0.4 6,036,697 - Pf6N2E2_5239 0.78 -0.9 6,036,697 - Pf6N2E2_5239 0.78 -0.9 6,036,697 - Pf6N2E2_5239 0.78 -3.1 6,036,697 - Pf6N2E2_5239 0.78 -0.6 6,036,697 - Pf6N2E2_5239 0.78 -0.6 6,036,697 - Pf6N2E2_5239 0.78 -0.7 6,036,697 - Pf6N2E2_5239 0.78 -0.8 6,036,897 + Pf6N2E2_5239 0.90 -1.0 6,036,897 + Pf6N2E2_5239 0.90 +0.0 6,036,897 + Pf6N2E2_5239 0.90 +1.0 6,036,897 + Pf6N2E2_5239 0.90 -0.9 6,036,898 - -2.5 6,036,898 - -0.1 6,036,898 - -1.1 6,036,898 - -1.3 6,037,014 + -1.7 6,037,076 + -1.2 6,037,076 + -0.9 6,037,076 + +0.7 6,037,076 + -0.3 6,037,076 + -2.2 6,037,077 - -0.1 6,037,077 - +0.3 6,037,077 - -0.3 6,037,077 - +1.3 6,037,143 - -0.8 6,037,154 + +0.4 6,037,154 + -0.3 6,037,154 + -1.1 6,037,154 + +0.5 6,037,154 + -0.8 6,037,154 + -0.9 6,037,155 - +2.0 6,037,155 - -0.6 6,037,155 - +0.9 6,037,155 - -0.7 6,037,158 - -0.0 6,037,158 - +0.4 6,037,267 + +0.1 6,037,290 - +1.0 6,037,307 + +0.4 6,037,308 - -0.1 6,037,365 + -0.4 6,037,498 + Pf6N2E2_5240 0.33 -0.7 6,037,623 + Pf6N2E2_5240 0.58 +0.4 6,037,624 - Pf6N2E2_5240 0.59 +0.9 6,037,696 + Pf6N2E2_5240 0.73 -0.6 6,037,697 - Pf6N2E2_5240 0.74 +0.0 6,037,750 + Pf6N2E2_5240 0.85 +0.2 6,037,750 + Pf6N2E2_5240 0.85 +0.5 6,037,750 + Pf6N2E2_5240 0.85 +0.7 6,037,750 + Pf6N2E2_5240 0.85 -0.1 6,037,750 + Pf6N2E2_5240 0.85 -0.6 6,037,750 + Pf6N2E2_5240 0.85 -1.0 6,037,750 + Pf6N2E2_5240 0.85 -1.7 6,037,751 - Pf6N2E2_5240 0.85 -0.1 6,037,751 - Pf6N2E2_5240 0.85 -0.3 6,037,768 + Pf6N2E2_5240 0.88 +0.8 6,037,769 - Pf6N2E2_5240 0.88 +1.6 6,037,769 - Pf6N2E2_5240 0.88 +0.4 6,037,783 - -0.4 6,037,783 - +1.0 6,037,783 - -0.3 6,037,783 - -0.4 6,037,783 - +0.6 6,037,783 - -0.5 6,037,846 - -0.6
Or see this region's nucleotide sequence