Experiment: N1B4wt with N2E2m denitrifying replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_483 and Pf6N2E2_484 are separated by 320 nucleotides Pf6N2E2_484 and Pf6N2E2_485 are separated by 196 nucleotides
Pf6N2E2_483 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 549,289 to 550,152
_483
Pf6N2E2_484 - Aldose 1-epimerase, at 550,473 to 551,342
_484
Pf6N2E2_485 - TRAP-type C4-dicarboxylate transport system, large permease component, at 551,539 to 552,819
_485
Position (kb)
550
551
552 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 549.533 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.596 kb on + strand, within Pf6N2E2_483 at 549.597 kb on - strand, within Pf6N2E2_483 at 549.597 kb on - strand, within Pf6N2E2_483 at 549.698 kb on + strand, within Pf6N2E2_483 at 549.911 kb on + strand, within Pf6N2E2_483 at 549.912 kb on - strand at 549.912 kb on - strand, within Pf6N2E2_483 at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.101 kb on - strand at 550.101 kb on - strand at 550.101 kb on - strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.107 kb on - strand at 550.107 kb on - strand at 550.107 kb on - strand at 550.107 kb on - strand at 550.237 kb on + strand at 550.243 kb on - strand at 550.266 kb on + strand at 550.267 kb on - strand at 550.267 kb on - strand at 550.267 kb on - strand at 550.267 kb on - strand at 550.288 kb on - strand at 550.288 kb on - strand at 550.474 kb on + strand at 550.621 kb on + strand, within Pf6N2E2_484 at 550.622 kb on - strand, within Pf6N2E2_484 at 550.633 kb on + strand, within Pf6N2E2_484 at 550.633 kb on + strand, within Pf6N2E2_484 at 550.634 kb on - strand, within Pf6N2E2_484 at 550.659 kb on + strand, within Pf6N2E2_484 at 550.659 kb on + strand, within Pf6N2E2_484 at 550.659 kb on + strand, within Pf6N2E2_484 at 550.659 kb on + strand, within Pf6N2E2_484 at 550.660 kb on - strand, within Pf6N2E2_484 at 550.660 kb on - strand, within Pf6N2E2_484 at 550.660 kb on - strand, within Pf6N2E2_484 at 550.671 kb on + strand, within Pf6N2E2_484 at 550.671 kb on + strand, within Pf6N2E2_484 at 550.671 kb on + strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.743 kb on + strand, within Pf6N2E2_484 at 550.870 kb on + strand, within Pf6N2E2_484 at 550.871 kb on - strand, within Pf6N2E2_484 at 551.540 kb on + strand at 551.541 kb on - strand at 551.541 kb on - strand at 551.582 kb on + strand at 551.582 kb on + strand at 551.582 kb on + strand at 551.582 kb on + strand at 551.582 kb on + strand at 551.582 kb on + strand at 551.582 kb on + strand at 551.583 kb on - strand at 551.583 kb on - strand at 551.583 kb on - strand at 551.583 kb on - strand at 551.583 kb on - strand at 551.583 kb on - strand at 551.618 kb on + strand at 551.618 kb on + strand at 551.879 kb on + strand, within Pf6N2E2_485 at 551.948 kb on + strand, within Pf6N2E2_485 at 551.948 kb on + strand, within Pf6N2E2_485 at 551.949 kb on - strand, within Pf6N2E2_485 at 551.949 kb on - strand, within Pf6N2E2_485 at 552.006 kb on + strand, within Pf6N2E2_485 at 552.007 kb on - strand, within Pf6N2E2_485 at 552.007 kb on - strand, within Pf6N2E2_485 at 552.007 kb on - strand, within Pf6N2E2_485 at 552.042 kb on - strand, within Pf6N2E2_485 at 552.042 kb on - strand, within Pf6N2E2_485 at 552.050 kb on + strand, within Pf6N2E2_485 at 552.224 kb on + strand, within Pf6N2E2_485 at 552.225 kb on - strand, within Pf6N2E2_485 at 552.225 kb on - strand, within Pf6N2E2_485 at 552.225 kb on - strand, within Pf6N2E2_485 at 552.225 kb on - strand, within Pf6N2E2_485 at 552.225 kb on - strand, within Pf6N2E2_485
Per-strain Table
Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate B remove 549,533 + Pf6N2E2_483 0.28 +0.4 549,548 + Pf6N2E2_483 0.30 +1.2 549,548 + Pf6N2E2_483 0.30 -0.2 549,548 + Pf6N2E2_483 0.30 -0.4 549,548 + Pf6N2E2_483 0.30 -0.4 549,549 - Pf6N2E2_483 0.30 -0.8 549,549 - Pf6N2E2_483 0.30 +0.7 549,549 - Pf6N2E2_483 0.30 -0.4 549,596 + Pf6N2E2_483 0.36 -0.6 549,597 - Pf6N2E2_483 0.36 -0.1 549,597 - Pf6N2E2_483 0.36 -0.0 549,698 + Pf6N2E2_483 0.47 +0.4 549,911 + Pf6N2E2_483 0.72 +0.7 549,912 - -0.0 549,912 - Pf6N2E2_483 0.72 -0.0 550,100 + -0.0 550,100 + -0.1 550,100 + +0.3 550,100 + -0.0 550,100 + +1.1 550,101 - -0.4 550,101 - +0.0 550,101 - +0.3 550,106 + +0.1 550,106 + -0.5 550,106 + +1.2 550,106 + -0.2 550,106 + -0.3 550,107 - -0.1 550,107 - +0.1 550,107 - -0.6 550,107 - -0.4 550,237 + -0.0 550,243 - -0.2 550,266 + -0.2 550,267 - +0.2 550,267 - -0.7 550,267 - +0.4 550,267 - -0.8 550,288 - +0.2 550,288 - +0.0 550,474 + +0.9 550,621 + Pf6N2E2_484 0.17 -0.6 550,622 - Pf6N2E2_484 0.17 +0.5 550,633 + Pf6N2E2_484 0.18 -0.6 550,633 + Pf6N2E2_484 0.18 -0.2 550,634 - Pf6N2E2_484 0.19 -0.2 550,659 + Pf6N2E2_484 0.21 -2.9 550,659 + Pf6N2E2_484 0.21 -1.8 550,659 + Pf6N2E2_484 0.21 -0.4 550,659 + Pf6N2E2_484 0.21 -0.4 550,660 - Pf6N2E2_484 0.21 +0.2 550,660 - Pf6N2E2_484 0.21 -0.1 550,660 - Pf6N2E2_484 0.21 -0.6 550,671 + Pf6N2E2_484 0.23 -0.8 550,671 + Pf6N2E2_484 0.23 -0.1 550,671 + Pf6N2E2_484 0.23 -0.3 550,672 - Pf6N2E2_484 0.23 +0.0 550,672 - Pf6N2E2_484 0.23 +0.3 550,672 - Pf6N2E2_484 0.23 +0.8 550,672 - Pf6N2E2_484 0.23 -0.8 550,672 - Pf6N2E2_484 0.23 -0.6 550,672 - Pf6N2E2_484 0.23 -0.0 550,672 - Pf6N2E2_484 0.23 -0.1 550,672 - Pf6N2E2_484 0.23 -0.1 550,743 + Pf6N2E2_484 0.31 -0.5 550,870 + Pf6N2E2_484 0.46 -0.8 550,871 - Pf6N2E2_484 0.46 -1.1 551,540 + -0.6 551,541 - -0.0 551,541 - -0.3 551,582 + +1.0 551,582 + +1.2 551,582 + -0.5 551,582 + +0.3 551,582 + +1.0 551,582 + +0.8 551,582 + -0.4 551,583 - +0.1 551,583 - +0.8 551,583 - -0.7 551,583 - -0.6 551,583 - -0.2 551,583 - +0.1 551,618 + -1.6 551,618 + -0.8 551,879 + Pf6N2E2_485 0.27 -0.1 551,948 + Pf6N2E2_485 0.32 +0.8 551,948 + Pf6N2E2_485 0.32 -0.5 551,949 - Pf6N2E2_485 0.32 +1.6 551,949 - Pf6N2E2_485 0.32 -0.4 552,006 + Pf6N2E2_485 0.36 +0.7 552,007 - Pf6N2E2_485 0.37 +0.2 552,007 - Pf6N2E2_485 0.37 +0.1 552,007 - Pf6N2E2_485 0.37 +0.5 552,042 - Pf6N2E2_485 0.39 -1.6 552,042 - Pf6N2E2_485 0.39 -0.8 552,050 + Pf6N2E2_485 0.40 +0.4 552,224 + Pf6N2E2_485 0.53 +0.3 552,225 - Pf6N2E2_485 0.54 +1.0 552,225 - Pf6N2E2_485 0.54 +0.3 552,225 - Pf6N2E2_485 0.54 +0.1 552,225 - Pf6N2E2_485 0.54 +1.0 552,225 - Pf6N2E2_485 0.54 -0.4
Or see this region's nucleotide sequence