Experiment: N1B4wt with N2E2m denitrifying replicate B 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_3659 and Pf6N2E2_3660 are separated by 221 nucleotides Pf6N2E2_3660 and Pf6N2E2_3661 are separated by 18 nucleotides Pf6N2E2_3661 and Pf6N2E2_3662 are separated by 192 nucleotides Pf6N2E2_3662 and Pf6N2E2_3663 are separated by 89 nucleotides 
        Pf6N2E2_3659 - Protein fixF, at 4,261,130 to 4,262,236 
        _3659 
         
        
        Pf6N2E2_3660 - Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase, at 4,262,458 to 4,263,090 
        _3660 
         
        
        Pf6N2E2_3661 - hypothetical protein, at 4,263,109 to 4,263,969 
        _3661 
         
        
        Pf6N2E2_3662 - hypothetical protein, at 4,264,162 to 4,264,302 
        _3662 
         
        
        Pf6N2E2_3663 - Glycosyl transferase, at 4,264,392 to 4,265,294 
        _3663 
         Position (kb) 4263 
4264 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 at 4262.144 kb on - strand at 4262.144 kb on - strand at 4262.144 kb on - strand at 4262.147 kb on + strand at 4262.147 kb on + strand at 4262.147 kb on + strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.190 kb on + strand at 4262.190 kb on + strand at 4262.191 kb on - strand at 4262.191 kb on - strand at 4262.252 kb on + strand at 4262.264 kb on - strand at 4262.279 kb on + strand at 4262.279 kb on + strand at 4262.295 kb on - strand at 4262.376 kb on - strand at 4262.377 kb on + strand at 4262.377 kb on + strand at 4262.377 kb on + strand at 4262.412 kb on + strand at 4262.412 kb on + strand at 4262.417 kb on + strand at 4262.417 kb on + strand at 4263.097 kb on + strand at 4263.097 kb on + strand at 4263.097 kb on + strand at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.493 kb on + strand, within Pf6N2E2_3661 at 4263.493 kb on + strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.563 kb on - strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.602 kb on - strand, within Pf6N2E2_3661 at 4263.608 kb on + strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.702 kb on - strand, within Pf6N2E2_3661 at 4263.720 kb on - strand, within Pf6N2E2_3661 at 4263.806 kb on + strand, within Pf6N2E2_3661 at 4263.962 kb on + strand at 4263.963 kb on - strand at 4263.995 kb on + strand at 4263.998 kb on - strand at 4263.998 kb on - strand at 4264.426 kb on + strand at 4264.427 kb on - strand at 4264.427 kb on - strand at 4264.475 kb on - strand at 4264.475 kb on - strand at 4264.495 kb on + strand, within Pf6N2E2_3663 at 4264.496 kb on - strand, within Pf6N2E2_3663 at 4264.667 kb on + strand, within Pf6N2E2_3663 at 4264.668 kb on - strand, within Pf6N2E2_3663 at 4264.668 kb on - strand, within Pf6N2E2_3663 at 4264.669 kb on + strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.768 kb on + strand, within Pf6N2E2_3663 at 4264.844 kb on - strand, within Pf6N2E2_3663 at 4264.941 kb on - strand, within Pf6N2E2_3663  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction N1B4wt with N2E2m denitrifying replicate B remove 4,262,144 -  -1.6 4,262,144 -  -3.3 4,262,144 -  -2.6 4,262,147 +  -2.0 4,262,147 +  -2.2 4,262,147 +  +1.0 4,262,148 -  +1.0 4,262,148 -  -0.8 4,262,148 -  -2.9 4,262,148 -  +1.0 4,262,148 -  -2.5 4,262,148 -  -2.8 4,262,190 +  -1.8 4,262,190 +  -1.0 4,262,191 -  -1.0 4,262,191 -  -2.9 4,262,252 +  -2.0 4,262,264 -  -0.8 4,262,279 +  -0.3 4,262,279 +  +1.0 4,262,295 -  +1.2 4,262,376 -  +0.2 4,262,377 +  -2.0 4,262,377 +  -1.6 4,262,377 +  -0.6 4,262,412 +  -1.2 4,262,412 +  -0.6 4,262,417 +  -3.6 4,262,417 +  -2.0 4,263,097 +  -0.2 4,263,097 +  -0.1 4,263,097 +  -0.1 4,263,216 -  Pf6N2E2_3661 0.12  -0.0 4,263,216 -  Pf6N2E2_3661 0.12  -0.6 4,263,216 -  Pf6N2E2_3661 0.12  -2.0 4,263,493 +  Pf6N2E2_3661 0.45  -2.2 4,263,493 +  Pf6N2E2_3661 0.45  -3.6 4,263,494 -  Pf6N2E2_3661 0.45  -0.0 4,263,494 -  Pf6N2E2_3661 0.45  -2.8 4,263,494 -  Pf6N2E2_3661 0.45  -2.7 4,263,563 -  Pf6N2E2_3661 0.53  -3.5 4,263,601 +  Pf6N2E2_3661 0.57  -3.2 4,263,601 +  Pf6N2E2_3661 0.57  -2.3 4,263,601 +  Pf6N2E2_3661 0.57  -3.2 4,263,602 -  Pf6N2E2_3661 0.57  -3.4 4,263,608 +  Pf6N2E2_3661 0.58  -3.5 4,263,609 -  Pf6N2E2_3661 0.58  -4.2 4,263,609 -  Pf6N2E2_3661 0.58  -2.0 4,263,609 -  Pf6N2E2_3661 0.58  -2.5 4,263,702 -  Pf6N2E2_3661 0.69  -4.7 4,263,720 -  Pf6N2E2_3661 0.71  -1.2 4,263,806 +  Pf6N2E2_3661 0.81  -2.2 4,263,962 +  -1.3 4,263,963 -  +1.0 4,263,995 +  -3.4 4,263,998 -  -1.8 4,263,998 -  -2.7 4,264,426 +  -2.0 4,264,427 -  -3.1 4,264,427 -  -1.2 4,264,475 -  -1.5 4,264,475 -  -3.9 4,264,495 +  Pf6N2E2_3663 0.11  -2.3 4,264,496 -  Pf6N2E2_3663 0.12  +1.0 4,264,667 +  Pf6N2E2_3663 0.30  -2.3 4,264,668 -  Pf6N2E2_3663 0.31  -3.4 4,264,668 -  Pf6N2E2_3663 0.31  -1.9 4,264,669 +  Pf6N2E2_3663 0.31  -3.3 4,264,670 -  Pf6N2E2_3663 0.31  -1.2 4,264,670 -  Pf6N2E2_3663 0.31  -2.0 4,264,670 -  Pf6N2E2_3663 0.31  -2.9 4,264,670 -  Pf6N2E2_3663 0.31  -3.0 4,264,670 -  Pf6N2E2_3663 0.31  -3.1 4,264,670 -  Pf6N2E2_3663 0.31  -2.9 4,264,768 +  Pf6N2E2_3663 0.42  -3.5 4,264,844 -  Pf6N2E2_3663 0.50  -2.1 4,264,941 -  Pf6N2E2_3663 0.61  -3.1 
Or see this region's nucleotide sequence