Experiment: N1B4wt with N2E2m denitrifying replicate B 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_1461 and Pf6N2E2_1462 are separated by 373 nucleotides Pf6N2E2_1462 and Pf6N2E2_1463 are separated by 93 nucleotides Pf6N2E2_1463 and Pf6N2E2_1464 are separated by 308 nucleotides Pf6N2E2_1464 and Pf6N2E2_1465 are separated by 99 nucleotides 
        Pf6N2E2_1461 - hypothetical protein, at 1,710,391 to 1,710,672 
        _1461 
         
        
        Pf6N2E2_1462 - Nicotinamidase/isochorismatase family protein, at 1,711,046 to 1,711,564 
        _1462 
         
        
        Pf6N2E2_1463 - Isochorismate pyruvate-lyase (EC 4.-.-.-), at 1,711,658 to 1,711,936 
        _1463 
         
        
        Pf6N2E2_1464 - hypothetical protein, at 1,712,245 to 1,712,763 
        _1464 
         
        
        Pf6N2E2_1465 - hypothetical protein, at 1,712,863 to 1,714,203 
        _1465 
         Position (kb) 1711 
1712 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 
3 
4 at 1710.662 kb on + strand at 1710.769 kb on + strand at 1710.828 kb on + strand at 1710.828 kb on + strand at 1710.959 kb on - strand at 1710.992 kb on + strand at 1711.195 kb on - strand, within Pf6N2E2_1462 at 1711.195 kb on - strand, within Pf6N2E2_1462 at 1711.195 kb on - strand, within Pf6N2E2_1462 at 1711.383 kb on - strand, within Pf6N2E2_1462 at 1711.387 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.454 kb on - strand, within Pf6N2E2_1462 at 1711.454 kb on - strand, within Pf6N2E2_1462 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.731 kb on - strand, within Pf6N2E2_1463 at 1711.731 kb on - strand, within Pf6N2E2_1463 at 1712.225 kb on + strand at 1712.226 kb on - strand at 1712.265 kb on + strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.527 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.677 kb on + strand, within Pf6N2E2_1464 at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.747 kb on - strand at 1712.779 kb on + strand at 1712.780 kb on - strand at 1712.780 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction N1B4wt with N2E2m denitrifying replicate B remove 1,710,662 +  -0.2 1,710,769 +  -0.2 1,710,828 +  +1.5 1,710,828 +  -0.0 1,710,959 -  +0.1 1,710,992 +  +0.4 1,711,195 -  Pf6N2E2_1462 0.29  -0.0 1,711,195 -  Pf6N2E2_1462 0.29  -0.0 1,711,195 -  Pf6N2E2_1462 0.29  +0.9 1,711,383 -  Pf6N2E2_1462 0.65  +0.5 1,711,387 +  Pf6N2E2_1462 0.66  -1.2 1,711,453 +  Pf6N2E2_1462 0.78  +0.1 1,711,453 +  Pf6N2E2_1462 0.78  -0.1 1,711,453 +  Pf6N2E2_1462 0.78  +0.3 1,711,454 -  Pf6N2E2_1462 0.79  +0.4 1,711,454 -  Pf6N2E2_1462 0.79  -0.3 1,711,730 +  Pf6N2E2_1463 0.26  -0.6 1,711,730 +  Pf6N2E2_1463 0.26  -0.0 1,711,730 +  Pf6N2E2_1463 0.26  -0.0 1,711,731 -  Pf6N2E2_1463 0.26  -2.1 1,711,731 -  Pf6N2E2_1463 0.26  -1.3 1,712,225 +  +3.8 1,712,226 -  -0.5 1,712,265 +  -0.4 1,712,266 -  -1.7 1,712,266 -  +0.1 1,712,266 -  +1.3 1,712,527 +  Pf6N2E2_1464 0.54  -0.7 1,712,632 +  Pf6N2E2_1464 0.75  -0.5 1,712,632 +  Pf6N2E2_1464 0.75  -0.6 1,712,633 -  Pf6N2E2_1464 0.75  -0.2 1,712,633 -  Pf6N2E2_1464 0.75  +1.3 1,712,633 -  Pf6N2E2_1464 0.75  -0.7 1,712,677 +  Pf6N2E2_1464 0.83  -0.5 1,712,746 +  -0.0 1,712,746 +  -0.0 1,712,746 +  -0.1 1,712,747 -  -1.8 1,712,779 +  -0.2 1,712,780 -  -0.0 1,712,780 -  -1.6 
Or see this region's nucleotide sequence