Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_786 and Pf1N1B4_787 are separated by 23 nucleotides Pf1N1B4_787 and Pf1N1B4_788 are separated by 207 nucleotides Pf1N1B4_788 and Pf1N1B4_789 are separated by 260 nucleotides
Pf1N1B4_786 - L-lysine permease, at 896,579 to 897,217
_786
Pf1N1B4_787 - Nudix hydrolase family protein PA3470, at 897,241 to 897,675
_787
Pf1N1B4_788 - Response regulator, at 897,883 to 899,241
_788
Pf1N1B4_789 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 899,502 to 901,802
_789
Position (kb)
897
898
899
900 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 896.970 kb on + strand at 897.063 kb on - strand, within Pf1N1B4_786 at 897.063 kb on - strand, within Pf1N1B4_786 at 897.138 kb on + strand, within Pf1N1B4_786 at 897.138 kb on + strand, within Pf1N1B4_786 at 897.138 kb on + strand, within Pf1N1B4_786 at 897.139 kb on - strand, within Pf1N1B4_786 at 897.139 kb on - strand, within Pf1N1B4_786 at 897.139 kb on - strand, within Pf1N1B4_786 at 897.164 kb on + strand at 897.281 kb on - strand at 897.281 kb on - strand at 897.409 kb on + strand at 897.410 kb on - strand, within Pf1N1B4_787 at 897.410 kb on - strand, within Pf1N1B4_787 at 897.746 kb on + strand at 897.747 kb on - strand at 897.765 kb on - strand at 897.798 kb on + strand at 897.812 kb on - strand at 897.822 kb on - strand at 897.823 kb on + strand at 897.824 kb on - strand at 897.824 kb on - strand at 897.824 kb on - strand at 897.824 kb on - strand at 897.824 kb on - strand at 897.884 kb on + strand at 897.884 kb on + strand at 897.884 kb on + strand at 897.884 kb on + strand at 897.885 kb on - strand at 897.885 kb on - strand at 897.885 kb on - strand at 897.885 kb on - strand at 897.934 kb on + strand at 897.934 kb on + strand at 897.934 kb on + strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.942 kb on - strand at 897.942 kb on - strand at 897.942 kb on - strand at 898.130 kb on + strand, within Pf1N1B4_788 at 898.130 kb on + strand, within Pf1N1B4_788 at 898.130 kb on + strand, within Pf1N1B4_788 at 898.131 kb on - strand, within Pf1N1B4_788 at 898.131 kb on - strand, within Pf1N1B4_788 at 898.131 kb on - strand, within Pf1N1B4_788 at 898.176 kb on - strand, within Pf1N1B4_788 at 898.191 kb on - strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand at 898.203 kb on - strand, within Pf1N1B4_788 at 898.203 kb on - strand, within Pf1N1B4_788 at 898.203 kb on - strand, within Pf1N1B4_788 at 898.520 kb on + strand, within Pf1N1B4_788 at 898.583 kb on + strand, within Pf1N1B4_788 at 898.679 kb on + strand, within Pf1N1B4_788 at 898.679 kb on + strand, within Pf1N1B4_788 at 898.679 kb on + strand at 898.679 kb on + strand, within Pf1N1B4_788 at 898.679 kb on + strand, within Pf1N1B4_788 at 898.679 kb on + strand, within Pf1N1B4_788 at 898.679 kb on + strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.680 kb on - strand, within Pf1N1B4_788 at 898.701 kb on - strand, within Pf1N1B4_788 at 898.701 kb on - strand, within Pf1N1B4_788 at 898.850 kb on + strand, within Pf1N1B4_788 at 898.910 kb on + strand, within Pf1N1B4_788 at 898.910 kb on + strand, within Pf1N1B4_788 at 898.911 kb on - strand, within Pf1N1B4_788 at 898.911 kb on - strand, within Pf1N1B4_788 at 898.911 kb on - strand, within Pf1N1B4_788 at 898.911 kb on - strand, within Pf1N1B4_788 at 899.197 kb on + strand at 899.198 kb on - strand at 899.198 kb on - strand at 899.208 kb on + strand at 899.209 kb on - strand at 899.210 kb on + strand at 899.210 kb on + strand at 899.210 kb on + strand at 899.210 kb on + strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.211 kb on - strand at 899.247 kb on + strand at 899.247 kb on + strand at 899.247 kb on + strand at 899.248 kb on - strand at 899.314 kb on + strand at 899.315 kb on - strand at 899.321 kb on + strand at 899.321 kb on + strand at 899.322 kb on - strand at 899.322 kb on - strand at 899.322 kb on - strand at 899.322 kb on - strand at 899.458 kb on - strand at 899.458 kb on - strand at 899.507 kb on + strand at 899.526 kb on - strand at 899.748 kb on + strand, within Pf1N1B4_789 at 899.868 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.135 kb on + strand, within Pf1N1B4_789 at 900.136 kb on - strand, within Pf1N1B4_789 at 900.136 kb on - strand, within Pf1N1B4_789 at 900.136 kb on - strand, within Pf1N1B4_789 at 900.136 kb on - strand, within Pf1N1B4_789 at 900.136 kb on - strand, within Pf1N1B4_789 at 900.136 kb on - strand, within Pf1N1B4_789 at 900.136 kb on - strand, within Pf1N1B4_789 at 900.136 kb on - strand, within Pf1N1B4_789 at 900.159 kb on + strand, within Pf1N1B4_789 at 900.159 kb on + strand, within Pf1N1B4_789 at 900.160 kb on - strand, within Pf1N1B4_789 at 900.160 kb on - strand, within Pf1N1B4_789 at 900.160 kb on - strand, within Pf1N1B4_789
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 896,970 + +1.6 897,063 - Pf1N1B4_786 0.76 -1.3 897,063 - Pf1N1B4_786 0.76 -0.4 897,138 + Pf1N1B4_786 0.87 -1.3 897,138 + Pf1N1B4_786 0.87 +0.3 897,138 + Pf1N1B4_786 0.87 +1.0 897,139 - Pf1N1B4_786 0.88 +0.7 897,139 - Pf1N1B4_786 0.88 +0.4 897,139 - Pf1N1B4_786 0.88 -1.0 897,164 + +0.0 897,281 - +2.8 897,281 - +0.1 897,409 + +0.0 897,410 - Pf1N1B4_787 0.39 -0.1 897,410 - Pf1N1B4_787 0.39 +0.8 897,746 + +0.6 897,747 - -1.0 897,765 - +0.6 897,798 + +2.6 897,812 - +0.7 897,822 - +0.6 897,823 + +1.6 897,824 - -1.0 897,824 - +2.0 897,824 - +0.4 897,824 - +0.2 897,824 - +0.3 897,884 + +0.0 897,884 + -0.7 897,884 + +0.0 897,884 + -2.0 897,885 - -1.0 897,885 - -0.7 897,885 - -0.4 897,885 - -0.6 897,934 + +0.4 897,934 + -2.3 897,934 + -0.8 897,935 - -2.0 897,935 - +0.2 897,935 - +0.0 897,935 - +0.7 897,935 - +0.5 897,935 - +0.6 897,935 - +0.0 897,942 - +1.2 897,942 - -0.6 897,942 - -0.4 898,130 + Pf1N1B4_788 0.18 -1.6 898,130 + Pf1N1B4_788 0.18 -1.6 898,130 + Pf1N1B4_788 0.18 +0.9 898,131 - Pf1N1B4_788 0.18 +1.8 898,131 - Pf1N1B4_788 0.18 +0.8 898,131 - Pf1N1B4_788 0.18 +1.4 898,176 - Pf1N1B4_788 0.22 -0.6 898,191 - Pf1N1B4_788 0.23 -0.2 898,202 + Pf1N1B4_788 0.23 -0.6 898,202 + Pf1N1B4_788 0.23 -0.4 898,202 + Pf1N1B4_788 0.23 -2.0 898,202 + Pf1N1B4_788 0.23 +0.0 898,202 + Pf1N1B4_788 0.23 -2.0 898,202 + +1.6 898,203 - Pf1N1B4_788 0.24 -0.3 898,203 - Pf1N1B4_788 0.24 -0.4 898,203 - Pf1N1B4_788 0.24 +0.0 898,520 + Pf1N1B4_788 0.47 -1.6 898,583 + Pf1N1B4_788 0.52 -2.2 898,679 + Pf1N1B4_788 0.59 +0.0 898,679 + Pf1N1B4_788 0.59 -1.1 898,679 + +1.0 898,679 + Pf1N1B4_788 0.59 -0.4 898,679 + Pf1N1B4_788 0.59 +1.3 898,679 + Pf1N1B4_788 0.59 -1.3 898,679 + Pf1N1B4_788 0.59 +1.3 898,680 - Pf1N1B4_788 0.59 -0.3 898,680 - Pf1N1B4_788 0.59 +1.2 898,680 - Pf1N1B4_788 0.59 -1.6 898,680 - Pf1N1B4_788 0.59 +0.8 898,680 - Pf1N1B4_788 0.59 +0.6 898,680 - Pf1N1B4_788 0.59 +0.0 898,680 - Pf1N1B4_788 0.59 -0.4 898,680 - Pf1N1B4_788 0.59 +2.3 898,680 - Pf1N1B4_788 0.59 +0.8 898,701 - Pf1N1B4_788 0.60 -1.3 898,701 - Pf1N1B4_788 0.60 +0.2 898,850 + Pf1N1B4_788 0.71 -0.1 898,910 + Pf1N1B4_788 0.76 -0.6 898,910 + Pf1N1B4_788 0.76 -0.8 898,911 - Pf1N1B4_788 0.76 +1.0 898,911 - Pf1N1B4_788 0.76 -1.0 898,911 - Pf1N1B4_788 0.76 -1.3 898,911 - Pf1N1B4_788 0.76 +1.6 899,197 + +0.8 899,198 - +0.0 899,198 - +0.0 899,208 + +1.6 899,209 - +0.6 899,210 + +0.0 899,210 + -1.0 899,210 + -1.0 899,210 + +0.0 899,211 - +0.6 899,211 - -0.6 899,211 - +1.0 899,211 - +0.4 899,211 - +0.5 899,211 - +0.8 899,211 - +0.2 899,211 - +0.6 899,211 - -0.7 899,211 - +0.0 899,247 + -0.0 899,247 + +2.0 899,247 + +0.4 899,248 - -1.0 899,314 + +0.0 899,315 - +0.8 899,321 + -2.3 899,321 + +1.0 899,322 - +1.0 899,322 - -2.7 899,322 - -0.4 899,322 - -2.0 899,458 - -2.0 899,458 - +0.4 899,507 + +1.6 899,526 - -0.6 899,748 + Pf1N1B4_789 0.11 -1.0 899,868 + Pf1N1B4_789 0.16 +0.5 900,135 + Pf1N1B4_789 0.28 +1.0 900,135 + Pf1N1B4_789 0.28 +0.6 900,135 + Pf1N1B4_789 0.28 +0.0 900,135 + Pf1N1B4_789 0.28 -0.6 900,135 + Pf1N1B4_789 0.28 +0.0 900,135 + Pf1N1B4_789 0.28 -1.3 900,135 + Pf1N1B4_789 0.28 -0.6 900,135 + Pf1N1B4_789 0.28 -0.7 900,135 + Pf1N1B4_789 0.28 +0.8 900,135 + Pf1N1B4_789 0.28 +0.6 900,135 + Pf1N1B4_789 0.28 -0.1 900,135 + Pf1N1B4_789 0.28 +1.4 900,135 + Pf1N1B4_789 0.28 +0.3 900,135 + Pf1N1B4_789 0.28 +0.0 900,136 - Pf1N1B4_789 0.28 +0.0 900,136 - Pf1N1B4_789 0.28 +1.2 900,136 - Pf1N1B4_789 0.28 +0.4 900,136 - Pf1N1B4_789 0.28 +0.7 900,136 - Pf1N1B4_789 0.28 -0.5 900,136 - Pf1N1B4_789 0.28 -1.7 900,136 - Pf1N1B4_789 0.28 +0.0 900,136 - Pf1N1B4_789 0.28 +0.2 900,159 + Pf1N1B4_789 0.29 -0.4 900,159 + Pf1N1B4_789 0.29 +0.8 900,160 - Pf1N1B4_789 0.29 +0.4 900,160 - Pf1N1B4_789 0.29 -1.8 900,160 - Pf1N1B4_789 0.29 +0.8
Or see this region's nucleotide sequence