Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_753 and Pf1N1B4_754 are separated by 88 nucleotides
Pf1N1B4_753 - hypothetical protein, at 856,306 to 856,773
_753
Pf1N1B4_754 - hypothetical protein, at 856,862 to 857,212
_754
Position (kb) 856
857 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 855.354 kb on - strand at 855.354 kb on - strand at 855.354 kb on - strand at 855.354 kb on - strand at 855.379 kb on + strand at 855.379 kb on + strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.436 kb on - strand at 855.446 kb on + strand at 855.447 kb on - strand at 855.447 kb on - strand at 855.454 kb on + strand at 855.455 kb on - strand at 855.455 kb on - strand at 855.498 kb on - strand at 855.543 kb on - strand at 855.543 kb on - strand at 855.543 kb on - strand at 855.578 kb on + strand at 855.579 kb on - strand at 855.579 kb on - strand at 855.606 kb on - strand at 855.707 kb on - strand at 855.766 kb on - strand at 855.958 kb on - strand at 856.114 kb on - strand at 856.114 kb on - strand at 856.450 kb on - strand, within Pf1N1B4_753 at 856.491 kb on + strand, within Pf1N1B4_753 at 856.491 kb on + strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand at 856.557 kb on + strand, within Pf1N1B4_753 at 856.558 kb on - strand, within Pf1N1B4_753 at 856.576 kb on + strand at 856.781 kb on + strand at 856.806 kb on + strand at 856.806 kb on + strand at 856.806 kb on + strand at 856.806 kb on + strand at 856.807 kb on - strand at 856.807 kb on - strand at 856.807 kb on - strand at 856.873 kb on + strand at 856.873 kb on + strand at 856.874 kb on - strand at 856.874 kb on - strand at 856.874 kb on - strand at 856.874 kb on - strand at 856.874 kb on - strand at 857.033 kb on + strand, within Pf1N1B4_754 at 857.034 kb on - strand, within Pf1N1B4_754 at 857.135 kb on - strand, within Pf1N1B4_754 at 857.241 kb on + strand at 857.242 kb on - strand at 857.242 kb on - strand at 857.292 kb on - strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.356 kb on - strand at 857.356 kb on - strand at 857.356 kb on - strand at 857.356 kb on - strand at 857.422 kb on - strand at 857.516 kb on + strand at 857.665 kb on + strand at 857.666 kb on - strand at 857.666 kb on - strand at 857.666 kb on - strand at 857.666 kb on - strand at 857.666 kb on - strand at 857.705 kb on - strand at 857.705 kb on - strand at 857.708 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 855,354 - +2.6 855,354 - +1.0 855,354 - +0.0 855,354 - +0.9 855,379 + -1.6 855,379 + +0.9 855,380 - -1.6 855,380 - -0.6 855,380 - +0.7 855,380 - -0.6 855,380 - +0.8 855,380 - +0.0 855,436 - +0.2 855,446 + -0.7 855,447 - +2.0 855,447 - +1.2 855,454 + -2.2 855,455 - +2.8 855,455 - -1.3 855,498 - +1.3 855,543 - -0.4 855,543 - -2.0 855,543 - +0.6 855,578 + +0.2 855,579 - +0.8 855,579 - -0.6 855,606 - -2.0 855,707 - -1.6 855,766 - +0.9 855,958 - +0.5 856,114 - +1.6 856,114 - +0.0 856,450 - Pf1N1B4_753 0.31 -1.0 856,491 + Pf1N1B4_753 0.40 +0.4 856,491 + Pf1N1B4_753 0.40 -2.6 856,492 - Pf1N1B4_753 0.40 -1.1 856,492 - Pf1N1B4_753 0.40 +0.0 856,492 - Pf1N1B4_753 0.40 +0.4 856,492 - Pf1N1B4_753 0.40 -1.0 856,492 - Pf1N1B4_753 0.40 +0.4 856,492 - -0.6 856,557 + Pf1N1B4_753 0.54 +0.0 856,558 - Pf1N1B4_753 0.54 -1.0 856,576 + +0.6 856,781 + +0.0 856,806 + -1.6 856,806 + -2.3 856,806 + +0.7 856,806 + -1.6 856,807 - -0.6 856,807 - -0.6 856,807 - -0.7 856,873 + +0.0 856,873 + +0.6 856,874 - +1.6 856,874 - +0.4 856,874 - -0.7 856,874 - -0.4 856,874 - +0.9 857,033 + Pf1N1B4_754 0.49 -1.0 857,034 - Pf1N1B4_754 0.49 +1.0 857,135 - Pf1N1B4_754 0.78 -1.0 857,241 + -0.6 857,242 - -0.7 857,242 - +0.3 857,292 - +1.0 857,355 + -0.3 857,355 + +1.0 857,355 + +0.0 857,355 + -0.4 857,355 + +0.7 857,355 + +2.3 857,355 + +1.0 857,356 - +1.8 857,356 - +1.6 857,356 - +0.0 857,356 - -0.7 857,422 - -0.5 857,516 + +0.9 857,665 + +1.3 857,666 - +0.0 857,666 - -0.1 857,666 - -1.6 857,666 - -0.5 857,666 - +1.0 857,705 - +0.0 857,705 - +0.9 857,708 - -1.6
Or see this region's nucleotide sequence