Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_613 and Pf1N1B4_614 are separated by 446 nucleotides Pf1N1B4_614 and Pf1N1B4_615 are separated by 35 nucleotides
Pf1N1B4_613 - Arginine/ornithine antiporter ArcD, at 707,879 to 709,306
_613
Pf1N1B4_614 - Arginine/ornithine antiporter ArcD, at 709,753 to 711,180
_614
Pf1N1B4_615 - Arginine deiminase (EC 3.5.3.6), at 711,216 to 712,472
_615
Position (kb) 709
710
711
712 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.083 kb on + strand, within Pf1N1B4_613 at 709.083 kb on + strand, within Pf1N1B4_613 at 709.083 kb on + strand at 709.083 kb on + strand at 709.083 kb on + strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand at 709.084 kb on - strand, within Pf1N1B4_613 at 709.335 kb on - strand at 709.374 kb on - strand at 709.385 kb on - strand at 709.422 kb on - strand at 709.425 kb on + strand at 709.426 kb on - strand at 709.473 kb on + strand at 709.473 kb on + strand at 709.474 kb on - strand at 709.529 kb on + strand at 709.540 kb on + strand at 709.541 kb on - strand at 709.541 kb on - strand at 709.643 kb on + strand at 709.643 kb on + strand at 709.652 kb on - strand at 709.882 kb on - strand at 709.976 kb on - strand, within Pf1N1B4_614 at 710.005 kb on + strand, within Pf1N1B4_614 at 710.065 kb on + strand, within Pf1N1B4_614 at 710.065 kb on + strand at 710.816 kb on - strand, within Pf1N1B4_614 at 710.830 kb on + strand, within Pf1N1B4_614 at 710.861 kb on - strand, within Pf1N1B4_614 at 710.911 kb on + strand, within Pf1N1B4_614 at 710.912 kb on - strand at 710.912 kb on - strand, within Pf1N1B4_614 at 710.971 kb on + strand, within Pf1N1B4_614 at 710.971 kb on + strand, within Pf1N1B4_614 at 710.990 kb on - strand, within Pf1N1B4_614 at 711.031 kb on + strand, within Pf1N1B4_614 at 711.031 kb on + strand, within Pf1N1B4_614 at 711.123 kb on - strand at 711.123 kb on - strand at 711.214 kb on + strand at 711.237 kb on + strand at 711.756 kb on + strand, within Pf1N1B4_615 at 711.757 kb on - strand, within Pf1N1B4_615
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 709,054 - Pf1N1B4_613 0.82 +1.9 709,054 - Pf1N1B4_613 0.82 +2.9 709,054 - Pf1N1B4_613 0.82 -1.6 709,054 - Pf1N1B4_613 0.82 +0.5 709,083 + Pf1N1B4_613 0.84 +0.7 709,083 + Pf1N1B4_613 0.84 +1.3 709,083 + +0.9 709,083 + -0.1 709,083 + Pf1N1B4_613 0.84 -1.1 709,084 - Pf1N1B4_613 0.84 -1.6 709,084 - Pf1N1B4_613 0.84 +0.7 709,084 - Pf1N1B4_613 0.84 -0.1 709,084 - Pf1N1B4_613 0.84 +0.8 709,084 - Pf1N1B4_613 0.84 -0.5 709,084 - +1.3 709,084 - Pf1N1B4_613 0.84 -0.6 709,335 - -1.1 709,374 - -1.1 709,385 - -0.2 709,422 - -0.1 709,425 + -1.1 709,426 - +0.6 709,473 + +0.9 709,473 + -0.5 709,474 - -2.9 709,529 + -2.1 709,540 + +0.7 709,541 - -1.1 709,541 - -0.1 709,643 + -0.6 709,643 + -0.1 709,652 - -0.6 709,882 - -1.1 709,976 - Pf1N1B4_614 0.16 -0.1 710,005 + Pf1N1B4_614 0.18 -0.8 710,065 + Pf1N1B4_614 0.22 -1.1 710,065 + -0.1 710,816 - Pf1N1B4_614 0.74 +0.8 710,830 + Pf1N1B4_614 0.75 -0.1 710,861 - Pf1N1B4_614 0.78 +0.9 710,911 + Pf1N1B4_614 0.81 -2.2 710,912 - -0.1 710,912 - Pf1N1B4_614 0.81 -1.1 710,971 + Pf1N1B4_614 0.85 -1.1 710,971 + Pf1N1B4_614 0.85 -2.9 710,990 - Pf1N1B4_614 0.87 +1.5 711,031 + Pf1N1B4_614 0.89 -0.1 711,031 + Pf1N1B4_614 0.89 -0.6 711,123 - -1.6 711,123 - -0.6 711,214 + -0.1 711,237 + -0.6 711,756 + Pf1N1B4_615 0.43 -0.7 711,757 - Pf1N1B4_615 0.43 -1.1
Or see this region's nucleotide sequence