Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5870 and Pf1N1B4_5871 are separated by 37 nucleotides Pf1N1B4_5871 and Pf1N1B4_5872 overlap by 4 nucleotides
Pf1N1B4_5870 - VgrG protein, at 303,515 to 305,557
_5870
Pf1N1B4_5871 - FIG00963848: hypothetical protein, at 305,595 to 306,290
_5871
Pf1N1B4_5872 - Rhs family protein, at 306,287 to 311,062
_5872
Position (kb)
306
307
308
309
310
311
312 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 305.485 kb on + strand at 305.485 kb on + strand at 305.489 kb on - strand at 305.930 kb on + strand, within Pf1N1B4_5871 at 305.930 kb on + strand, within Pf1N1B4_5871 at 305.930 kb on + strand, within Pf1N1B4_5871 at 305.995 kb on - strand, within Pf1N1B4_5871 at 306.002 kb on + strand, within Pf1N1B4_5871 at 306.165 kb on + strand, within Pf1N1B4_5871 at 306.165 kb on + strand, within Pf1N1B4_5871 at 306.286 kb on + strand at 306.287 kb on - strand at 306.407 kb on + strand at 306.497 kb on + strand at 306.655 kb on - strand at 306.705 kb on - strand at 306.799 kb on - strand, within Pf1N1B4_5872 at 306.844 kb on + strand, within Pf1N1B4_5872 at 306.978 kb on - strand, within Pf1N1B4_5872 at 306.978 kb on - strand, within Pf1N1B4_5872 at 307.094 kb on + strand, within Pf1N1B4_5872 at 307.094 kb on + strand, within Pf1N1B4_5872 at 307.094 kb on + strand, within Pf1N1B4_5872 at 307.248 kb on + strand, within Pf1N1B4_5872 at 308.016 kb on - strand, within Pf1N1B4_5872 at 308.116 kb on + strand, within Pf1N1B4_5872 at 308.117 kb on - strand, within Pf1N1B4_5872 at 308.390 kb on + strand, within Pf1N1B4_5872 at 308.390 kb on + strand, within Pf1N1B4_5872 at 308.472 kb on - strand, within Pf1N1B4_5872 at 308.569 kb on - strand at 308.569 kb on - strand at 308.604 kb on - strand, within Pf1N1B4_5872 at 308.667 kb on - strand, within Pf1N1B4_5872 at 308.701 kb on + strand at 308.701 kb on + strand, within Pf1N1B4_5872 at 308.780 kb on + strand, within Pf1N1B4_5872 at 308.781 kb on - strand, within Pf1N1B4_5872 at 308.786 kb on + strand, within Pf1N1B4_5872 at 308.787 kb on - strand at 308.906 kb on + strand, within Pf1N1B4_5872 at 308.906 kb on + strand, within Pf1N1B4_5872 at 308.922 kb on + strand, within Pf1N1B4_5872 at 309.038 kb on + strand, within Pf1N1B4_5872 at 309.039 kb on - strand, within Pf1N1B4_5872 at 309.039 kb on - strand, within Pf1N1B4_5872 at 309.233 kb on + strand, within Pf1N1B4_5872 at 309.296 kb on + strand, within Pf1N1B4_5872 at 309.434 kb on + strand, within Pf1N1B4_5872 at 309.434 kb on + strand, within Pf1N1B4_5872 at 309.434 kb on + strand, within Pf1N1B4_5872 at 309.434 kb on + strand, within Pf1N1B4_5872 at 309.435 kb on - strand, within Pf1N1B4_5872 at 309.435 kb on - strand at 309.555 kb on - strand, within Pf1N1B4_5872 at 309.738 kb on - strand at 309.813 kb on - strand, within Pf1N1B4_5872 at 309.875 kb on + strand at 309.876 kb on - strand, within Pf1N1B4_5872 at 309.876 kb on - strand, within Pf1N1B4_5872 at 309.876 kb on - strand, within Pf1N1B4_5872 at 310.031 kb on + strand, within Pf1N1B4_5872 at 310.032 kb on - strand, within Pf1N1B4_5872 at 310.170 kb on - strand, within Pf1N1B4_5872 at 310.170 kb on - strand, within Pf1N1B4_5872 at 310.281 kb on - strand at 310.281 kb on - strand, within Pf1N1B4_5872 at 310.287 kb on - strand, within Pf1N1B4_5872 at 310.287 kb on - strand, within Pf1N1B4_5872 at 310.287 kb on - strand, within Pf1N1B4_5872 at 310.356 kb on - strand, within Pf1N1B4_5872 at 310.356 kb on - strand, within Pf1N1B4_5872 at 310.356 kb on - strand, within Pf1N1B4_5872 at 310.359 kb on - strand, within Pf1N1B4_5872 at 310.401 kb on - strand, within Pf1N1B4_5872 at 310.430 kb on + strand, within Pf1N1B4_5872 at 310.430 kb on + strand, within Pf1N1B4_5872 at 310.431 kb on - strand, within Pf1N1B4_5872 at 310.440 kb on - strand, within Pf1N1B4_5872 at 310.503 kb on - strand, within Pf1N1B4_5872 at 310.503 kb on - strand, within Pf1N1B4_5872 at 310.524 kb on + strand, within Pf1N1B4_5872 at 310.525 kb on - strand, within Pf1N1B4_5872 at 310.525 kb on - strand, within Pf1N1B4_5872 at 310.529 kb on + strand at 310.529 kb on + strand, within Pf1N1B4_5872 at 310.529 kb on + strand, within Pf1N1B4_5872 at 310.529 kb on + strand, within Pf1N1B4_5872 at 310.530 kb on - strand, within Pf1N1B4_5872 at 310.530 kb on - strand, within Pf1N1B4_5872 at 310.547 kb on + strand, within Pf1N1B4_5872 at 310.548 kb on - strand, within Pf1N1B4_5872 at 310.548 kb on - strand, within Pf1N1B4_5872 at 310.548 kb on - strand at 310.613 kb on + strand at 310.628 kb on - strand at 310.731 kb on + strand at 310.819 kb on - strand at 310.829 kb on - strand at 310.935 kb on + strand at 310.939 kb on - strand at 310.943 kb on + strand at 311.690 kb on - strand at 311.700 kb on - strand at 311.741 kb on + strand at 311.777 kb on - strand at 311.972 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 305,485 + -0.5 305,485 + -1.8 305,489 - +0.0 305,930 + Pf1N1B4_5871 0.48 +1.1 305,930 + Pf1N1B4_5871 0.48 +1.4 305,930 + Pf1N1B4_5871 0.48 -1.2 305,995 - Pf1N1B4_5871 0.57 +0.6 306,002 + Pf1N1B4_5871 0.58 +1.2 306,165 + Pf1N1B4_5871 0.82 -2.2 306,165 + Pf1N1B4_5871 0.82 +1.4 306,286 + -0.8 306,287 - -1.8 306,407 + +0.4 306,497 + -0.8 306,655 - +2.4 306,705 - +0.4 306,799 - Pf1N1B4_5872 0.11 -0.6 306,844 + Pf1N1B4_5872 0.12 +0.2 306,978 - Pf1N1B4_5872 0.14 -1.1 306,978 - Pf1N1B4_5872 0.14 -1.2 307,094 + Pf1N1B4_5872 0.17 +0.5 307,094 + Pf1N1B4_5872 0.17 +1.1 307,094 + Pf1N1B4_5872 0.17 -0.2 307,248 + Pf1N1B4_5872 0.20 +0.6 308,016 - Pf1N1B4_5872 0.36 +0.2 308,116 + Pf1N1B4_5872 0.38 -1.0 308,117 - Pf1N1B4_5872 0.38 +2.2 308,390 + Pf1N1B4_5872 0.44 -0.2 308,390 + Pf1N1B4_5872 0.44 -1.8 308,472 - Pf1N1B4_5872 0.46 +0.3 308,569 - -0.2 308,569 - +0.8 308,604 - Pf1N1B4_5872 0.49 -0.6 308,667 - Pf1N1B4_5872 0.50 +0.8 308,701 + +0.8 308,701 + Pf1N1B4_5872 0.51 -0.2 308,780 + Pf1N1B4_5872 0.52 -1.4 308,781 - Pf1N1B4_5872 0.52 +1.8 308,786 + Pf1N1B4_5872 0.52 -0.8 308,787 - -0.2 308,906 + Pf1N1B4_5872 0.55 +0.6 308,906 + Pf1N1B4_5872 0.55 +1.8 308,922 + Pf1N1B4_5872 0.55 -2.5 309,038 + Pf1N1B4_5872 0.58 +0.4 309,039 - Pf1N1B4_5872 0.58 -0.2 309,039 - Pf1N1B4_5872 0.58 -0.6 309,233 + Pf1N1B4_5872 0.62 +0.0 309,296 + Pf1N1B4_5872 0.63 -0.2 309,434 + Pf1N1B4_5872 0.66 -0.2 309,434 + Pf1N1B4_5872 0.66 +1.4 309,434 + Pf1N1B4_5872 0.66 -0.8 309,434 + Pf1N1B4_5872 0.66 -0.2 309,435 - Pf1N1B4_5872 0.66 -1.2 309,435 - +1.8 309,555 - Pf1N1B4_5872 0.68 +0.1 309,738 - -0.2 309,813 - Pf1N1B4_5872 0.74 +0.4 309,875 + +0.4 309,876 - Pf1N1B4_5872 0.75 +0.2 309,876 - Pf1N1B4_5872 0.75 -0.8 309,876 - Pf1N1B4_5872 0.75 -0.2 310,031 + Pf1N1B4_5872 0.78 +0.5 310,032 - Pf1N1B4_5872 0.78 -0.1 310,170 - Pf1N1B4_5872 0.81 +1.2 310,170 - Pf1N1B4_5872 0.81 +2.4 310,281 - -0.2 310,281 - Pf1N1B4_5872 0.84 +0.6 310,287 - Pf1N1B4_5872 0.84 -0.5 310,287 - Pf1N1B4_5872 0.84 -1.8 310,287 - Pf1N1B4_5872 0.84 +0.5 310,356 - Pf1N1B4_5872 0.85 -1.5 310,356 - Pf1N1B4_5872 0.85 -1.2 310,356 - Pf1N1B4_5872 0.85 -0.8 310,359 - Pf1N1B4_5872 0.85 -1.0 310,401 - Pf1N1B4_5872 0.86 +0.4 310,430 + Pf1N1B4_5872 0.87 +0.2 310,430 + Pf1N1B4_5872 0.87 +1.0 310,431 - Pf1N1B4_5872 0.87 -0.6 310,440 - Pf1N1B4_5872 0.87 +1.0 310,503 - Pf1N1B4_5872 0.88 +0.8 310,503 - Pf1N1B4_5872 0.88 -0.2 310,524 + Pf1N1B4_5872 0.89 -0.2 310,525 - Pf1N1B4_5872 0.89 -0.8 310,525 - Pf1N1B4_5872 0.89 +3.0 310,529 + -1.2 310,529 + Pf1N1B4_5872 0.89 +1.4 310,529 + Pf1N1B4_5872 0.89 +0.2 310,529 + Pf1N1B4_5872 0.89 +0.8 310,530 - Pf1N1B4_5872 0.89 +0.4 310,530 - Pf1N1B4_5872 0.89 +2.4 310,547 + Pf1N1B4_5872 0.89 +2.1 310,548 - Pf1N1B4_5872 0.89 +0.4 310,548 - Pf1N1B4_5872 0.89 +0.0 310,548 - +0.4 310,613 + +0.0 310,628 - -0.2 310,731 + +1.0 310,819 - -0.6 310,829 - +0.4 310,935 + +0.4 310,939 - -1.2 310,943 + +0.8 311,690 - +1.8 311,700 - -0.1 311,741 + +0.4 311,777 - -0.2 311,972 + -1.2
Or see this region's nucleotide sequence