Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotides Pf1N1B4_5849 and Pf1N1B4_5850 overlap by 50 nucleotides Pf1N1B4_5850 and Pf1N1B4_5851 are separated by 247 nucleotides
Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852
_5848
Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337
_5849
Pf1N1B4_5850 - hypothetical protein, at 283,288 to 283,509
_5850
Pf1N1B4_5851 - Transcriptional regulator, RpiR family, at 283,757 to 284,611
_5851
Position (kb) 283
284 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand at 282.393 kb on + strand at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.495 kb on + strand, within Pf1N1B4_5848 at 282.496 kb on - strand at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.504 kb on - strand, within Pf1N1B4_5848 at 282.504 kb on - strand, within Pf1N1B4_5848 at 282.623 kb on + strand at 282.692 kb on + strand, within Pf1N1B4_5848 at 282.692 kb on + strand, within Pf1N1B4_5848 at 282.693 kb on - strand, within Pf1N1B4_5848 at 282.888 kb on + strand at 282.889 kb on - strand at 283.020 kb on - strand at 283.020 kb on - strand, within Pf1N1B4_5849 at 283.530 kb on + strand at 283.530 kb on + strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.683 kb on + strand at 283.683 kb on + strand at 283.801 kb on + strand at 283.824 kb on + strand at 283.825 kb on - strand at 284.036 kb on - strand, within Pf1N1B4_5851 at 284.058 kb on - strand, within Pf1N1B4_5851 at 284.058 kb on - strand, within Pf1N1B4_5851 at 284.118 kb on + strand, within Pf1N1B4_5851 at 284.119 kb on - strand, within Pf1N1B4_5851 at 284.119 kb on - strand, within Pf1N1B4_5851 at 284.402 kb on + strand, within Pf1N1B4_5851 at 284.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 282,393 + Pf1N1B4_5848 0.39 +0.4 282,393 + Pf1N1B4_5848 0.39 -0.9 282,393 + Pf1N1B4_5848 0.39 +0.4 282,393 + -0.2 282,393 + +0.5 282,393 + Pf1N1B4_5848 0.39 -0.6 282,393 + Pf1N1B4_5848 0.39 -0.9 282,394 - Pf1N1B4_5848 0.39 -0.8 282,394 - Pf1N1B4_5848 0.39 +0.5 282,394 - +0.8 282,394 - Pf1N1B4_5848 0.39 -3.2 282,394 - Pf1N1B4_5848 0.39 +0.3 282,394 - -0.2 282,394 - Pf1N1B4_5848 0.39 -0.6 282,495 + Pf1N1B4_5848 0.53 +0.5 282,496 - -0.2 282,503 + Pf1N1B4_5848 0.54 -0.7 282,503 + Pf1N1B4_5848 0.54 +0.6 282,503 + Pf1N1B4_5848 0.54 -0.2 282,503 + Pf1N1B4_5848 0.54 -0.8 282,503 + Pf1N1B4_5848 0.54 -0.2 282,504 - Pf1N1B4_5848 0.54 +1.1 282,504 - Pf1N1B4_5848 0.54 -1.8 282,623 + -1.2 282,692 + Pf1N1B4_5848 0.79 -1.9 282,692 + Pf1N1B4_5848 0.79 +0.1 282,693 - Pf1N1B4_5848 0.79 -1.4 282,888 + +0.4 282,889 - +0.5 283,020 - -0.2 283,020 - Pf1N1B4_5849 0.35 +1.0 283,530 + -1.5 283,530 + -1.2 283,531 - +1.4 283,531 - -0.8 283,683 + -0.2 283,683 + +1.4 283,801 + +0.8 283,824 + -0.7 283,825 - -0.1 284,036 - Pf1N1B4_5851 0.33 -0.4 284,058 - Pf1N1B4_5851 0.35 +0.6 284,058 - Pf1N1B4_5851 0.35 +1.0 284,118 + Pf1N1B4_5851 0.42 -0.5 284,119 - Pf1N1B4_5851 0.42 +0.4 284,119 - Pf1N1B4_5851 0.42 -0.2 284,402 + Pf1N1B4_5851 0.75 +2.9 284,403 - +0.8
Or see this region's nucleotide sequence