Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_565 and Pf1N1B4_566 are separated by 128 nucleotides Pf1N1B4_566 and Pf1N1B4_567 are separated by 83 nucleotides Pf1N1B4_567 and Pf1N1B4_568 are separated by 133 nucleotides
Pf1N1B4_565 - Fatty acid desaturase, at 661,519 to 662,460
_565
Pf1N1B4_566 - hypothetical protein, at 662,589 to 662,717
_566
Pf1N1B4_567 - Histone acetyltransferase HPA2 and related acetyltransferases, at 662,801 to 663,220
_567
Pf1N1B4_568 - Peptide deformylase (EC 3.5.1.88), at 663,354 to 663,893
_568
Position (kb)
662
663 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 661.641 kb on - strand, within Pf1N1B4_565 at 661.659 kb on - strand, within Pf1N1B4_565 at 661.659 kb on - strand, within Pf1N1B4_565 at 661.787 kb on + strand, within Pf1N1B4_565 at 661.787 kb on + strand, within Pf1N1B4_565 at 661.788 kb on - strand, within Pf1N1B4_565 at 661.788 kb on - strand at 661.788 kb on - strand, within Pf1N1B4_565 at 661.788 kb on - strand, within Pf1N1B4_565 at 661.788 kb on - strand, within Pf1N1B4_565 at 661.788 kb on - strand, within Pf1N1B4_565 at 661.790 kb on + strand, within Pf1N1B4_565 at 661.790 kb on + strand, within Pf1N1B4_565 at 661.790 kb on + strand, within Pf1N1B4_565 at 661.790 kb on + strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.841 kb on + strand, within Pf1N1B4_565 at 661.911 kb on + strand, within Pf1N1B4_565 at 662.088 kb on - strand at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.450 kb on + strand at 662.524 kb on - strand at 662.524 kb on - strand at 662.532 kb on - strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand at 662.703 kb on - strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.717 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.736 kb on + strand at 662.736 kb on + strand at 662.736 kb on + strand at 662.737 kb on - strand at 662.737 kb on - strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.744 kb on + strand at 662.745 kb on - strand at 662.745 kb on - strand at 662.766 kb on + strand at 662.767 kb on - strand at 662.767 kb on - strand at 662.768 kb on + strand at 662.768 kb on + strand at 662.769 kb on - strand at 662.769 kb on - strand at 662.769 kb on - strand at 662.816 kb on + strand at 662.816 kb on + strand at 662.816 kb on + strand at 662.817 kb on - strand at 662.817 kb on - strand at 662.943 kb on + strand at 662.943 kb on + strand, within Pf1N1B4_567 at 662.943 kb on + strand, within Pf1N1B4_567 at 662.943 kb on + strand, within Pf1N1B4_567 at 663.102 kb on - strand at 663.102 kb on - strand, within Pf1N1B4_567 at 663.102 kb on - strand, within Pf1N1B4_567 at 663.118 kb on + strand, within Pf1N1B4_567 at 663.119 kb on - strand, within Pf1N1B4_567 at 663.277 kb on - strand at 663.439 kb on - strand, within Pf1N1B4_568
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 661,641 - Pf1N1B4_565 0.13 -1.7 661,659 - Pf1N1B4_565 0.15 -0.1 661,659 - Pf1N1B4_565 0.15 +0.4 661,787 + Pf1N1B4_565 0.28 +0.5 661,787 + Pf1N1B4_565 0.28 +1.4 661,788 - Pf1N1B4_565 0.29 -0.6 661,788 - -1.1 661,788 - Pf1N1B4_565 0.29 +0.9 661,788 - Pf1N1B4_565 0.29 -0.1 661,788 - Pf1N1B4_565 0.29 +1.5 661,788 - Pf1N1B4_565 0.29 +1.6 661,790 + Pf1N1B4_565 0.29 -1.7 661,790 + Pf1N1B4_565 0.29 -0.4 661,790 + Pf1N1B4_565 0.29 -1.7 661,790 + Pf1N1B4_565 0.29 -0.1 661,791 - Pf1N1B4_565 0.29 -2.1 661,791 - Pf1N1B4_565 0.29 -2.1 661,791 - Pf1N1B4_565 0.29 -1.1 661,791 - Pf1N1B4_565 0.29 -0.1 661,791 - Pf1N1B4_565 0.29 -1.3 661,791 - Pf1N1B4_565 0.29 -2.3 661,791 - Pf1N1B4_565 0.29 +0.7 661,841 + Pf1N1B4_565 0.34 -0.1 661,911 + Pf1N1B4_565 0.42 +0.9 662,088 - +0.9 662,354 + Pf1N1B4_565 0.89 +0.7 662,354 + Pf1N1B4_565 0.89 +0.5 662,354 + Pf1N1B4_565 0.89 +0.7 662,354 + Pf1N1B4_565 0.89 +0.8 662,354 + Pf1N1B4_565 0.89 +0.8 662,355 - Pf1N1B4_565 0.89 +0.9 662,355 - Pf1N1B4_565 0.89 +0.2 662,355 - Pf1N1B4_565 0.89 +0.6 662,450 + -0.9 662,524 - +0.1 662,524 - +0.9 662,532 - -1.4 662,702 + Pf1N1B4_566 0.88 -1.1 662,702 + Pf1N1B4_566 0.88 -1.1 662,702 + Pf1N1B4_566 0.88 +1.5 662,702 + Pf1N1B4_566 0.88 -2.4 662,702 + Pf1N1B4_566 0.88 +0.2 662,702 + Pf1N1B4_566 0.88 -0.4 662,702 + Pf1N1B4_566 0.88 -2.1 662,702 + Pf1N1B4_566 0.88 -1.3 662,702 + Pf1N1B4_566 0.88 -1.1 662,702 + -0.1 662,702 + Pf1N1B4_566 0.88 +1.2 662,702 + Pf1N1B4_566 0.88 +0.2 662,702 + -1.1 662,702 + Pf1N1B4_566 0.88 -1.1 662,703 - Pf1N1B4_566 0.88 -0.1 662,703 - Pf1N1B4_566 0.88 -0.8 662,703 - Pf1N1B4_566 0.88 -0.2 662,703 - Pf1N1B4_566 0.88 -2.4 662,703 - Pf1N1B4_566 0.88 +0.3 662,703 - Pf1N1B4_566 0.88 -1.3 662,703 - Pf1N1B4_566 0.88 -0.2 662,703 - Pf1N1B4_566 0.88 -2.1 662,703 - Pf1N1B4_566 0.88 -1.7 662,703 - -2.4 662,703 - -0.1 662,706 + -1.1 662,706 + +0.3 662,706 + -0.3 662,706 + -0.9 662,706 + +0.9 662,707 - +0.7 662,707 - +0.4 662,707 - +1.9 662,707 - +0.9 662,707 - +0.8 662,707 - +0.5 662,707 - -1.1 662,707 - -0.7 662,707 - -1.1 662,717 + -1.7 662,726 + -1.3 662,726 + +0.9 662,726 + -0.6 662,726 + +0.9 662,727 - -0.8 662,727 - +0.5 662,727 - +0.5 662,727 - +0.1 662,727 - -1.4 662,727 - +1.5 662,727 - +0.7 662,727 - -0.7 662,727 - +1.3 662,727 - -1.1 662,736 + +2.2 662,736 + +0.7 662,736 + +0.6 662,737 - -0.5 662,737 - +0.6 662,738 + +0.5 662,738 + +0.7 662,738 + -0.1 662,738 + +0.9 662,738 + -0.1 662,738 + -1.7 662,738 + +0.9 662,738 + -0.5 662,738 + +1.2 662,738 + -1.7 662,739 - -1.7 662,739 - +1.5 662,739 - +0.9 662,739 - -0.7 662,739 - -0.1 662,739 - +0.7 662,739 - -2.7 662,739 - -0.7 662,739 - -0.8 662,739 - -0.4 662,739 - -0.1 662,739 - -0.7 662,739 - +0.6 662,739 - -0.1 662,739 - -0.5 662,739 - -0.9 662,739 - -1.1 662,739 - +1.3 662,739 - +0.9 662,739 - +0.2 662,739 - +2.7 662,739 - +0.3 662,739 - -0.9 662,744 + -1.3 662,745 - +1.5 662,745 - -0.1 662,766 + +0.9 662,767 - +0.1 662,767 - +0.7 662,768 + -1.1 662,768 + -1.1 662,769 - +2.2 662,769 - -0.1 662,769 - -1.9 662,816 + -0.1 662,816 + -0.7 662,816 + -0.1 662,817 - -2.1 662,817 - -0.7 662,943 + -0.1 662,943 + Pf1N1B4_567 0.34 -0.5 662,943 + Pf1N1B4_567 0.34 +0.3 662,943 + Pf1N1B4_567 0.34 +1.3 663,102 - +1.5 663,102 - Pf1N1B4_567 0.72 +1.2 663,102 - Pf1N1B4_567 0.72 +0.2 663,118 + Pf1N1B4_567 0.75 -1.7 663,119 - Pf1N1B4_567 0.76 -0.1 663,277 - +0.5 663,439 - Pf1N1B4_568 0.16 +0.9
Or see this region's nucleotide sequence