Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_55 and Pf1N1B4_56 are separated by 732 nucleotides Pf1N1B4_56 and Pf1N1B4_57 are separated by 260 nucleotides
Pf1N1B4_55 - FIG00961388: hypothetical protein, at 59,566 to 59,892
_55
Pf1N1B4_56 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 60,625 to 60,987
_56
Pf1N1B4_57 - tRNA dihydrouridine synthase A, at 61,248 to 62,186
_57
Position (kb)
60
61 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 59.663 kb on + strand, within Pf1N1B4_55 at 59.798 kb on + strand at 59.799 kb on - strand, within Pf1N1B4_55 at 59.799 kb on - strand, within Pf1N1B4_55 at 59.799 kb on - strand, within Pf1N1B4_55 at 59.811 kb on + strand, within Pf1N1B4_55 at 59.812 kb on - strand, within Pf1N1B4_55 at 59.851 kb on - strand, within Pf1N1B4_55 at 59.851 kb on - strand, within Pf1N1B4_55 at 59.895 kb on + strand at 59.903 kb on + strand at 59.904 kb on - strand at 59.995 kb on + strand at 60.096 kb on + strand at 60.096 kb on + strand at 60.096 kb on + strand at 60.096 kb on + strand at 60.097 kb on - strand at 60.097 kb on - strand at 60.097 kb on - strand at 60.097 kb on - strand at 60.097 kb on - strand at 60.144 kb on + strand at 60.145 kb on - strand at 60.227 kb on - strand at 60.241 kb on + strand at 60.241 kb on + strand at 60.341 kb on + strand at 60.375 kb on + strand at 60.375 kb on + strand at 60.389 kb on + strand at 60.429 kb on + strand at 60.436 kb on + strand at 60.437 kb on - strand at 60.437 kb on - strand at 60.487 kb on + strand at 60.487 kb on + strand at 60.543 kb on + strand at 60.543 kb on + strand at 60.543 kb on + strand at 60.599 kb on + strand at 60.599 kb on + strand at 60.599 kb on + strand at 60.599 kb on + strand at 60.599 kb on + strand at 60.599 kb on + strand at 60.603 kb on + strand at 60.603 kb on + strand at 60.603 kb on + strand at 60.620 kb on + strand at 60.620 kb on + strand at 60.620 kb on + strand at 60.620 kb on + strand at 60.620 kb on + strand at 60.621 kb on - strand at 60.621 kb on - strand at 60.624 kb on + strand at 60.624 kb on + strand at 60.624 kb on + strand at 60.625 kb on - strand at 60.625 kb on - strand at 60.727 kb on + strand, within Pf1N1B4_56 at 60.728 kb on - strand, within Pf1N1B4_56 at 60.802 kb on + strand, within Pf1N1B4_56 at 60.802 kb on + strand, within Pf1N1B4_56 at 60.802 kb on + strand, within Pf1N1B4_56 at 60.802 kb on + strand, within Pf1N1B4_56 at 60.802 kb on + strand, within Pf1N1B4_56 at 60.802 kb on + strand, within Pf1N1B4_56 at 60.803 kb on - strand, within Pf1N1B4_56 at 60.803 kb on - strand, within Pf1N1B4_56 at 60.858 kb on + strand, within Pf1N1B4_56 at 60.985 kb on + strand at 60.985 kb on + strand at 60.985 kb on + strand at 60.985 kb on + strand at 61.049 kb on + strand at 61.314 kb on + strand at 61.315 kb on - strand at 61.520 kb on + strand, within Pf1N1B4_57 at 61.520 kb on + strand at 61.520 kb on + strand, within Pf1N1B4_57 at 61.520 kb on + strand, within Pf1N1B4_57 at 61.520 kb on + strand, within Pf1N1B4_57 at 61.521 kb on - strand, within Pf1N1B4_57 at 61.521 kb on - strand, within Pf1N1B4_57 at 61.521 kb on - strand, within Pf1N1B4_57 at 61.628 kb on + strand, within Pf1N1B4_57 at 61.628 kb on + strand, within Pf1N1B4_57 at 61.644 kb on + strand, within Pf1N1B4_57 at 61.700 kb on + strand, within Pf1N1B4_57 at 61.700 kb on + strand, within Pf1N1B4_57 at 61.700 kb on + strand, within Pf1N1B4_57 at 61.701 kb on - strand, within Pf1N1B4_57 at 61.820 kb on + strand, within Pf1N1B4_57 at 61.821 kb on - strand, within Pf1N1B4_57 at 61.917 kb on + strand, within Pf1N1B4_57 at 61.917 kb on + strand, within Pf1N1B4_57 at 61.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 59,663 + Pf1N1B4_55 0.30 +1.8 59,798 + -0.2 59,799 - Pf1N1B4_55 0.71 -0.2 59,799 - Pf1N1B4_55 0.71 +0.3 59,799 - Pf1N1B4_55 0.71 +0.3 59,811 + Pf1N1B4_55 0.75 -0.6 59,812 - Pf1N1B4_55 0.75 -0.8 59,851 - Pf1N1B4_55 0.87 -0.6 59,851 - Pf1N1B4_55 0.87 +1.1 59,895 + -0.2 59,903 + +1.4 59,904 - -0.6 59,995 + -1.2 60,096 + +0.0 60,096 + +0.9 60,096 + +0.4 60,096 + -0.8 60,097 - +2.1 60,097 - +0.2 60,097 - +1.4 60,097 - +0.4 60,097 - +1.4 60,144 + -0.2 60,145 - +0.4 60,227 - +0.3 60,241 + -1.2 60,241 + +0.8 60,341 + -0.2 60,375 + +1.1 60,375 + -0.7 60,389 + +0.8 60,429 + -0.2 60,436 + -1.0 60,437 - -0.8 60,437 - -0.2 60,487 + -0.8 60,487 + -0.9 60,543 + -2.2 60,543 + -0.2 60,543 + -1.0 60,599 + +0.6 60,599 + +1.8 60,599 + -0.0 60,599 + +0.2 60,599 + +1.6 60,599 + +1.6 60,603 + -1.0 60,603 + +1.4 60,603 + +0.1 60,620 + +0.5 60,620 + -0.6 60,620 + +1.4 60,620 + -1.2 60,620 + +1.4 60,621 - -2.6 60,621 - -0.3 60,624 + -0.2 60,624 + +0.6 60,624 + -0.6 60,625 - -1.0 60,625 - -1.8 60,727 + Pf1N1B4_56 0.28 +1.4 60,728 - Pf1N1B4_56 0.28 -0.8 60,802 + Pf1N1B4_56 0.49 -0.2 60,802 + Pf1N1B4_56 0.49 +0.8 60,802 + Pf1N1B4_56 0.49 -0.8 60,802 + Pf1N1B4_56 0.49 -0.2 60,802 + Pf1N1B4_56 0.49 -1.2 60,802 + Pf1N1B4_56 0.49 -0.9 60,803 - Pf1N1B4_56 0.49 -1.8 60,803 - Pf1N1B4_56 0.49 +0.4 60,858 + Pf1N1B4_56 0.64 -0.7 60,985 + -2.4 60,985 + -1.0 60,985 + +1.6 60,985 + +2.6 61,049 + +0.5 61,314 + -0.2 61,315 - +0.3 61,520 + Pf1N1B4_57 0.29 +0.8 61,520 + -1.2 61,520 + Pf1N1B4_57 0.29 +1.4 61,520 + Pf1N1B4_57 0.29 -0.2 61,520 + Pf1N1B4_57 0.29 -2.0 61,521 - Pf1N1B4_57 0.29 -1.6 61,521 - Pf1N1B4_57 0.29 +0.3 61,521 - Pf1N1B4_57 0.29 -2.2 61,628 + Pf1N1B4_57 0.40 -1.8 61,628 + Pf1N1B4_57 0.40 +1.1 61,644 + Pf1N1B4_57 0.42 +0.8 61,700 + Pf1N1B4_57 0.48 -0.6 61,700 + Pf1N1B4_57 0.48 -0.2 61,700 + Pf1N1B4_57 0.48 -0.2 61,701 - Pf1N1B4_57 0.48 -1.1 61,820 + Pf1N1B4_57 0.61 +0.1 61,821 - Pf1N1B4_57 0.61 +0.9 61,917 + Pf1N1B4_57 0.71 -0.2 61,917 + Pf1N1B4_57 0.71 -1.6 61,918 - -1.2
Or see this region's nucleotide sequence