Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5590 and Pf1N1B4_5591 are separated by 143 nucleotides Pf1N1B4_5591 and Pf1N1B4_5592 are separated by 15 nucleotides Pf1N1B4_5592 and Pf1N1B4_5593 overlap by 32 nucleotides
Pf1N1B4_5590 - Trans-feruloyl-CoA synthase (EC 6.2.1.34), at 11,783 to 13,666
_5590
Pf1N1B4_5591 - Vanillin dehydrogenase (Hydroxybenzaldehyde dehydrogenase) (EC 1.2.1.28), at 13,810 to 15,258
_5591
Pf1N1B4_5592 - hypothetical protein, at 15,274 to 15,417
_5592
Pf1N1B4_5593 - 4-hydroxycinnamoyl CoA hydratase/lyase (Enoyl-CoA hydratase/lyase) (EC 4.2.1.17), at 15,386 to 16,216
_5593
Position (kb) 13
14
15
16 Strain fitness (log2 ratio) -2
-1
0
1 at 13.288 kb on - strand, within Pf1N1B4_5590 at 13.419 kb on + strand, within Pf1N1B4_5590 at 13.559 kb on + strand at 13.560 kb on - strand at 13.560 kb on - strand at 13.604 kb on + strand at 13.737 kb on + strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.965 kb on + strand, within Pf1N1B4_5591 at 14.184 kb on + strand, within Pf1N1B4_5591 at 14.878 kb on + strand, within Pf1N1B4_5591 at 14.878 kb on + strand at 14.878 kb on + strand, within Pf1N1B4_5591 at 14.879 kb on - strand, within Pf1N1B4_5591 at 15.273 kb on - strand at 15.291 kb on - strand, within Pf1N1B4_5592 at 15.508 kb on - strand, within Pf1N1B4_5593 at 15.708 kb on + strand, within Pf1N1B4_5593 at 15.708 kb on + strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.903 kb on + strand, within Pf1N1B4_5593 at 15.904 kb on - strand, within Pf1N1B4_5593 at 15.993 kb on + strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 16.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 13,288 - Pf1N1B4_5590 0.80 +1.1 13,419 + Pf1N1B4_5590 0.87 -0.9 13,559 + +0.3 13,560 - +0.2 13,560 - +0.8 13,604 + +0.8 13,737 + -2.2 13,744 - +0.8 13,744 - -0.6 13,744 - +0.8 13,744 - +1.4 13,744 - -1.2 13,965 + Pf1N1B4_5591 0.11 -1.8 14,184 + Pf1N1B4_5591 0.26 -1.2 14,878 + Pf1N1B4_5591 0.74 -0.6 14,878 + +0.4 14,878 + Pf1N1B4_5591 0.74 -0.2 14,879 - Pf1N1B4_5591 0.74 -0.6 15,273 - -0.2 15,291 - Pf1N1B4_5592 0.12 -0.6 15,508 - Pf1N1B4_5593 0.15 -0.8 15,708 + Pf1N1B4_5593 0.39 +0.3 15,708 + Pf1N1B4_5593 0.39 -2.2 15,709 - Pf1N1B4_5593 0.39 +0.2 15,709 - Pf1N1B4_5593 0.39 -0.2 15,709 - Pf1N1B4_5593 0.39 -1.8 15,709 - Pf1N1B4_5593 0.39 -0.2 15,709 - Pf1N1B4_5593 0.39 -0.1 15,903 + Pf1N1B4_5593 0.62 -0.2 15,904 - Pf1N1B4_5593 0.62 +0.4 15,993 + Pf1N1B4_5593 0.73 +0.6 15,994 - Pf1N1B4_5593 0.73 -0.6 15,994 - Pf1N1B4_5593 0.73 -1.2 15,994 - Pf1N1B4_5593 0.73 +0.8 16,206 + -0.2
Or see this region's nucleotide sequence