Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5500

Experiment: Dimethyl Sulfoxide 6.5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5499 and Pf1N1B4_5500 are separated by 177 nucleotidesPf1N1B4_5500 and Pf1N1B4_5501 are separated by 209 nucleotidesPf1N1B4_5501 and Pf1N1B4_5502 overlap by 35 nucleotides Pf1N1B4_5499 - hypothetical protein, at 247,890 to 249,695 _5499 Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862 _5500 Pf1N1B4_5501 - hypothetical protein, at 251,072 to 251,413 _5501 Pf1N1B4_5502 - hypothetical protein, at 251,379 to 252,542 _5502 Position (kb) 249 250 251Strain fitness (log2 ratio) -2 -1 0 1 2at 248.900 kb on + strand, within Pf1N1B4_5499at 248.971 kb on + strand, within Pf1N1B4_5499at 248.972 kb on - strand, within Pf1N1B4_5499at 248.972 kb on - strand, within Pf1N1B4_5499at 248.972 kb on - strand, within Pf1N1B4_5499at 249.004 kb on - strandat 249.070 kb on + strand, within Pf1N1B4_5499at 249.070 kb on + strand, within Pf1N1B4_5499at 249.070 kb on + strand, within Pf1N1B4_5499at 249.071 kb on - strand, within Pf1N1B4_5499at 249.232 kb on + strand, within Pf1N1B4_5499at 249.232 kb on + strandat 249.232 kb on + strand, within Pf1N1B4_5499at 249.233 kb on - strand, within Pf1N1B4_5499at 249.233 kb on - strandat 249.246 kb on - strand, within Pf1N1B4_5499at 249.246 kb on - strand, within Pf1N1B4_5499at 249.274 kb on + strand, within Pf1N1B4_5499at 249.450 kb on - strand, within Pf1N1B4_5499at 249.450 kb on - strand, within Pf1N1B4_5499at 249.450 kb on - strand, within Pf1N1B4_5499at 249.560 kb on + strandat 249.571 kb on + strandat 249.571 kb on + strandat 249.571 kb on + strandat 249.572 kb on - strandat 249.572 kb on - strandat 249.623 kb on + strandat 249.627 kb on + strandat 249.627 kb on + strandat 249.627 kb on + strandat 249.628 kb on - strandat 249.682 kb on + strandat 249.683 kb on - strandat 249.683 kb on - strandat 249.742 kb on - strandat 249.840 kb on - strandat 249.840 kb on - strandat 249.880 kb on + strandat 249.880 kb on + strandat 249.880 kb on + strandat 249.880 kb on + strandat 249.880 kb on + strandat 249.880 kb on + strandat 249.881 kb on - strandat 249.881 kb on - strandat 249.881 kb on - strandat 249.881 kb on - strandat 249.881 kb on - strandat 249.881 kb on - strandat 249.881 kb on - strandat 249.881 kb on - strandat 249.881 kb on - strandat 249.882 kb on + strandat 249.883 kb on - strandat 249.883 kb on - strandat 249.883 kb on - strandat 250.144 kb on + strand, within Pf1N1B4_5500at 250.145 kb on - strand, within Pf1N1B4_5500at 250.224 kb on + strand, within Pf1N1B4_5500at 250.224 kb on + strandat 250.224 kb on + strand, within Pf1N1B4_5500at 250.224 kb on + strandat 250.224 kb on + strand, within Pf1N1B4_5500at 250.224 kb on + strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.225 kb on - strand, within Pf1N1B4_5500at 250.268 kb on - strand, within Pf1N1B4_5500at 250.365 kb on + strand, within Pf1N1B4_5500at 250.365 kb on + strand, within Pf1N1B4_5500at 250.366 kb on - strand, within Pf1N1B4_5500at 250.366 kb on - strand, within Pf1N1B4_5500at 250.366 kb on - strand, within Pf1N1B4_5500at 250.366 kb on - strand, within Pf1N1B4_5500at 250.429 kb on + strand, within Pf1N1B4_5500at 250.429 kb on + strand, within Pf1N1B4_5500at 250.430 kb on - strand, within Pf1N1B4_5500at 250.430 kb on - strand, within Pf1N1B4_5500at 250.430 kb on - strand, within Pf1N1B4_5500at 250.430 kb on - strand, within Pf1N1B4_5500at 250.689 kb on - strand, within Pf1N1B4_5500at 250.689 kb on - strand, within Pf1N1B4_5500at 250.737 kb on - strand, within Pf1N1B4_5500at 250.757 kb on - strandat 250.758 kb on + strand, within Pf1N1B4_5500at 250.758 kb on + strandat 250.758 kb on + strand, within Pf1N1B4_5500at 250.758 kb on + strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.759 kb on - strand, within Pf1N1B4_5500at 250.771 kb on - strandat 250.805 kb on - strandat 250.807 kb on + strandat 250.807 kb on + strandat 250.807 kb on + strandat 250.870 kb on + strandat 250.870 kb on + strandat 250.871 kb on - strandat 250.871 kb on - strandat 250.871 kb on - strandat 250.976 kb on + strandat 250.976 kb on + strandat 250.976 kb on + strandat 250.977 kb on - strandat 250.977 kb on - strandat 251.024 kb on + strandat 251.024 kb on + strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.025 kb on - strandat 251.067 kb on + strandat 251.067 kb on + strandat 251.067 kb on + strandat 251.068 kb on - strandat 251.260 kb on + strand, within Pf1N1B4_5501at 251.261 kb on - strand, within Pf1N1B4_5501at 251.261 kb on - strand, within Pf1N1B4_5501at 251.279 kb on + strand, within Pf1N1B4_5501at 251.302 kb on + strand, within Pf1N1B4_5501at 251.302 kb on + strand, within Pf1N1B4_5501at 251.302 kb on + strand, within Pf1N1B4_5501at 251.302 kb on + strand, within Pf1N1B4_5501at 251.303 kb on - strand, within Pf1N1B4_5501at 251.303 kb on - strand, within Pf1N1B4_5501at 251.303 kb on - strand, within Pf1N1B4_5501at 251.411 kb on + strandat 251.411 kb on + strandat 251.412 kb on - strandat 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.517 kb on + strand, within Pf1N1B4_5502at 251.518 kb on - strand, within Pf1N1B4_5502at 251.518 kb on - strand, within Pf1N1B4_5502at 251.534 kb on + strand, within Pf1N1B4_5502at 251.590 kb on + strandat 251.739 kb on + strand, within Pf1N1B4_5502at 251.739 kb on + strand, within Pf1N1B4_5502at 251.739 kb on + strand, within Pf1N1B4_5502at 251.740 kb on - strand, within Pf1N1B4_5502at 251.798 kb on + strand, within Pf1N1B4_5502

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Per-strain Table

Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol%
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248,900 + Pf1N1B4_5499 0.56 +1.0
248,971 + Pf1N1B4_5499 0.60 -0.3
248,972 - Pf1N1B4_5499 0.60 -0.7
248,972 - Pf1N1B4_5499 0.60 +0.4
248,972 - Pf1N1B4_5499 0.60 -1.0
249,004 - +2.0
249,070 + Pf1N1B4_5499 0.65 -2.3
249,070 + Pf1N1B4_5499 0.65 -1.8
249,070 + Pf1N1B4_5499 0.65 -1.4
249,071 - Pf1N1B4_5499 0.65 -0.6
249,232 + Pf1N1B4_5499 0.74 -1.6
249,232 + -0.6
249,232 + Pf1N1B4_5499 0.74 +0.3
249,233 - Pf1N1B4_5499 0.74 +1.0
249,233 - -1.0
249,246 - Pf1N1B4_5499 0.75 -0.3
249,246 - Pf1N1B4_5499 0.75 +1.4
249,274 + Pf1N1B4_5499 0.77 +0.1
249,450 - Pf1N1B4_5499 0.86 +0.6
249,450 - Pf1N1B4_5499 0.86 +0.5
249,450 - Pf1N1B4_5499 0.86 +1.3
249,560 + -0.7
249,571 + +1.6
249,571 + -0.0
249,571 + -0.0
249,572 - -0.6
249,572 - -0.0
249,623 + -0.4
249,627 + +1.0
249,627 + -0.0
249,627 + +0.7
249,628 - +0.4
249,682 + +2.3
249,683 - +0.6
249,683 - +0.4
249,742 - -0.6
249,840 - -0.0
249,840 - +1.0
249,880 + +0.2
249,880 + +1.0
249,880 + -0.9
249,880 + -0.7
249,880 + -0.4
249,880 + +0.7
249,881 - -0.6
249,881 - +0.2
249,881 - +1.0
249,881 - -0.3
249,881 - -0.0
249,881 - -0.3
249,881 - -1.6
249,881 - +0.6
249,881 - -0.0
249,882 + -1.0
249,883 - +2.0
249,883 - -0.2
249,883 - -0.4
250,144 + Pf1N1B4_5500 0.27 +0.3
250,145 - Pf1N1B4_5500 0.27 -1.6
250,224 + Pf1N1B4_5500 0.35 -0.0
250,224 + -0.0
250,224 + Pf1N1B4_5500 0.35 -1.6
250,224 + -0.6
250,224 + Pf1N1B4_5500 0.35 -1.6
250,224 + Pf1N1B4_5500 0.35 -0.3
250,225 - Pf1N1B4_5500 0.36 -0.5
250,225 - Pf1N1B4_5500 0.36 -0.3
250,225 - Pf1N1B4_5500 0.36 +2.0
250,225 - Pf1N1B4_5500 0.36 -0.6
250,225 - Pf1N1B4_5500 0.36 +1.6
250,225 - Pf1N1B4_5500 0.36 -0.7
250,225 - Pf1N1B4_5500 0.36 -0.3
250,268 - Pf1N1B4_5500 0.40 -0.0
250,365 + Pf1N1B4_5500 0.50 -1.3
250,365 + Pf1N1B4_5500 0.50 +0.7
250,366 - Pf1N1B4_5500 0.50 -0.6
250,366 - Pf1N1B4_5500 0.50 +1.0
250,366 - Pf1N1B4_5500 0.50 +0.6
250,366 - Pf1N1B4_5500 0.50 -0.0
250,429 + Pf1N1B4_5500 0.56 -0.3
250,429 + Pf1N1B4_5500 0.56 -2.0
250,430 - Pf1N1B4_5500 0.56 -0.5
250,430 - Pf1N1B4_5500 0.56 +0.4
250,430 - Pf1N1B4_5500 0.56 +2.0
250,430 - Pf1N1B4_5500 0.56 +1.0
250,689 - Pf1N1B4_5500 0.82 -2.0
250,689 - Pf1N1B4_5500 0.82 +0.1
250,737 - Pf1N1B4_5500 0.87 -0.0
250,757 - -0.0
250,758 + Pf1N1B4_5500 0.89 +1.3
250,758 + -1.6
250,758 + Pf1N1B4_5500 0.89 +2.0
250,758 + Pf1N1B4_5500 0.89 -0.6
250,759 - Pf1N1B4_5500 0.89 -1.0
250,759 - Pf1N1B4_5500 0.89 +1.0
250,759 - Pf1N1B4_5500 0.89 -0.5
250,759 - Pf1N1B4_5500 0.89 +0.7
250,759 - Pf1N1B4_5500 0.89 +0.6
250,759 - Pf1N1B4_5500 0.89 +0.8
250,759 - Pf1N1B4_5500 0.89 +0.6
250,759 - Pf1N1B4_5500 0.89 +0.4
250,771 - +0.6
250,805 - -0.3
250,807 + -1.2
250,807 + +2.3
250,807 + -1.0
250,870 + -0.0
250,870 + -1.0
250,871 - -0.0
250,871 - -0.4
250,871 - -0.0
250,976 + -0.0
250,976 + -0.4
250,976 + -1.6
250,977 - -1.6
250,977 - -0.2
251,024 + -0.2
251,024 + -0.7
251,025 - -0.0
251,025 - -0.3
251,025 - +0.7
251,025 - +0.3
251,025 - -0.0
251,025 - +1.0
251,067 + +0.4
251,067 + -1.0
251,067 + +1.3
251,068 - +1.3
251,260 + Pf1N1B4_5501 0.55 -0.6
251,261 - Pf1N1B4_5501 0.55 +0.4
251,261 - Pf1N1B4_5501 0.55 +0.7
251,279 + Pf1N1B4_5501 0.61 +0.0
251,302 + Pf1N1B4_5501 0.67 -1.6
251,302 + Pf1N1B4_5501 0.67 +0.6
251,302 + Pf1N1B4_5501 0.67 -0.7
251,302 + Pf1N1B4_5501 0.67 -0.0
251,303 - Pf1N1B4_5501 0.68 +0.4
251,303 - Pf1N1B4_5501 0.68 -0.0
251,303 - Pf1N1B4_5501 0.68 +1.4
251,411 + -0.0
251,411 + -2.0
251,412 - +0.8
251,517 + Pf1N1B4_5502 0.12 +0.4
251,517 + Pf1N1B4_5502 0.12 -0.7
251,517 + Pf1N1B4_5502 0.12 +0.5
251,517 + Pf1N1B4_5502 0.12 +0.4
251,517 + Pf1N1B4_5502 0.12 +0.3
251,517 + Pf1N1B4_5502 0.12 -1.0
251,518 - Pf1N1B4_5502 0.12 -0.0
251,518 - Pf1N1B4_5502 0.12 +0.2
251,534 + Pf1N1B4_5502 0.13 +0.3
251,590 + -0.0
251,739 + Pf1N1B4_5502 0.31 +0.6
251,739 + Pf1N1B4_5502 0.31 -1.3
251,739 + Pf1N1B4_5502 0.31 -1.4
251,740 - Pf1N1B4_5502 0.31 +0.4
251,798 + Pf1N1B4_5502 0.36 -0.5

Or see this region's nucleotide sequence