Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5281 and Pf1N1B4_5282 are separated by 212 nucleotides Pf1N1B4_5282 and Pf1N1B4_5283 are separated by 47 nucleotides Pf1N1B4_5283 and Pf1N1B4_5284 are separated by 327 nucleotides
Pf1N1B4_5281 - hypothetical protein, at 35,503 to 36,150
_5281
Pf1N1B4_5282 - Phage protein, at 36,363 to 36,533
_5282
Pf1N1B4_5283 - hypothetical protein, at 36,581 to 36,943
_5283
Pf1N1B4_5284 - hypothetical protein, at 37,271 to 37,441
_5284
Position (kb) 36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 35.609 kb on + strand, within Pf1N1B4_5281 at 35.609 kb on + strand at 35.610 kb on - strand, within Pf1N1B4_5281 at 35.610 kb on - strand, within Pf1N1B4_5281 at 35.610 kb on - strand, within Pf1N1B4_5281 at 35.610 kb on - strand, within Pf1N1B4_5281 at 35.632 kb on + strand, within Pf1N1B4_5281 at 35.632 kb on + strand, within Pf1N1B4_5281 at 35.633 kb on - strand, within Pf1N1B4_5281 at 35.633 kb on - strand, within Pf1N1B4_5281 at 35.633 kb on - strand, within Pf1N1B4_5281 at 35.633 kb on - strand, within Pf1N1B4_5281 at 35.700 kb on + strand, within Pf1N1B4_5281 at 35.700 kb on + strand, within Pf1N1B4_5281 at 35.701 kb on - strand at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.715 kb on + strand, within Pf1N1B4_5281 at 35.715 kb on + strand, within Pf1N1B4_5281 at 35.715 kb on + strand at 35.716 kb on - strand, within Pf1N1B4_5281 at 35.716 kb on - strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.793 kb on - strand, within Pf1N1B4_5281 at 35.857 kb on + strand, within Pf1N1B4_5281 at 35.858 kb on - strand, within Pf1N1B4_5281 at 35.858 kb on - strand, within Pf1N1B4_5281 at 35.897 kb on + strand, within Pf1N1B4_5281 at 35.897 kb on + strand, within Pf1N1B4_5281 at 35.897 kb on + strand, within Pf1N1B4_5281 at 35.898 kb on - strand, within Pf1N1B4_5281 at 35.898 kb on - strand, within Pf1N1B4_5281 at 36.150 kb on + strand at 36.151 kb on - strand at 36.151 kb on - strand at 36.171 kb on + strand at 36.171 kb on + strand at 36.223 kb on + strand at 36.223 kb on + strand at 36.223 kb on + strand at 36.223 kb on + strand at 36.223 kb on + strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.342 kb on - strand at 36.342 kb on - strand at 36.342 kb on - strand at 36.342 kb on - strand at 36.389 kb on - strand, within Pf1N1B4_5282 at 36.417 kb on + strand, within Pf1N1B4_5282 at 36.417 kb on + strand at 36.417 kb on + strand, within Pf1N1B4_5282 at 36.417 kb on + strand, within Pf1N1B4_5282 at 36.446 kb on + strand, within Pf1N1B4_5282 at 36.446 kb on + strand, within Pf1N1B4_5282 at 36.446 kb on + strand, within Pf1N1B4_5282 at 36.447 kb on - strand, within Pf1N1B4_5282 at 36.447 kb on - strand, within Pf1N1B4_5282 at 36.531 kb on + strand at 36.633 kb on - strand at 36.635 kb on + strand, within Pf1N1B4_5283 at 36.635 kb on + strand, within Pf1N1B4_5283 at 36.635 kb on + strand, within Pf1N1B4_5283 at 36.635 kb on + strand, within Pf1N1B4_5283 at 36.890 kb on - strand, within Pf1N1B4_5283 at 36.941 kb on + strand at 36.941 kb on + strand at 36.942 kb on - strand at 36.942 kb on - strand at 36.960 kb on + strand at 36.961 kb on - strand at 37.001 kb on + strand at 37.001 kb on + strand at 37.001 kb on + strand at 37.052 kb on - strand at 37.079 kb on + strand at 37.080 kb on - strand at 37.080 kb on - strand at 37.170 kb on + strand at 37.171 kb on - strand at 37.198 kb on + strand at 37.199 kb on - strand at 37.201 kb on + strand at 37.201 kb on + strand at 37.201 kb on + strand at 37.202 kb on - strand at 37.202 kb on - strand at 37.202 kb on - strand at 37.202 kb on - strand at 37.248 kb on - strand at 37.295 kb on + strand, within Pf1N1B4_5284 at 37.316 kb on + strand, within Pf1N1B4_5284 at 37.356 kb on + strand, within Pf1N1B4_5284 at 37.356 kb on + strand, within Pf1N1B4_5284 at 37.357 kb on - strand, within Pf1N1B4_5284 at 37.368 kb on + strand, within Pf1N1B4_5284 at 37.369 kb on - strand at 37.369 kb on - strand, within Pf1N1B4_5284 at 37.476 kb on - strand at 37.484 kb on - strand at 37.519 kb on - strand at 37.535 kb on + strand at 37.536 kb on - strand at 37.552 kb on + strand at 37.599 kb on + strand at 37.599 kb on + strand at 37.599 kb on + strand at 37.600 kb on - strand at 37.600 kb on - strand at 37.686 kb on + strand at 37.687 kb on - strand at 37.714 kb on - strand at 37.765 kb on + strand at 37.766 kb on - strand at 37.766 kb on - strand at 37.796 kb on + strand at 37.796 kb on + strand at 37.797 kb on - strand at 37.817 kb on + strand at 37.817 kb on + strand at 37.818 kb on - strand at 37.821 kb on + strand at 37.822 kb on - strand at 37.822 kb on - strand at 37.837 kb on + strand at 37.837 kb on + strand at 37.837 kb on + strand at 37.837 kb on + strand at 37.838 kb on - strand at 37.878 kb on + strand at 37.878 kb on + strand at 37.878 kb on + strand at 37.878 kb on + strand at 37.878 kb on + strand at 37.879 kb on - strand at 37.883 kb on + strand at 37.895 kb on + strand at 37.896 kb on - strand at 37.901 kb on + strand at 37.901 kb on + strand at 37.902 kb on - strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.938 kb on + strand at 37.938 kb on + strand at 37.938 kb on + strand at 37.938 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 35,609 + Pf1N1B4_5281 0.16 -0.6 35,609 + +0.4 35,610 - Pf1N1B4_5281 0.17 -1.4 35,610 - Pf1N1B4_5281 0.17 -0.2 35,610 - Pf1N1B4_5281 0.17 -0.3 35,610 - Pf1N1B4_5281 0.17 +0.8 35,632 + Pf1N1B4_5281 0.20 -1.0 35,632 + Pf1N1B4_5281 0.20 +1.8 35,633 - Pf1N1B4_5281 0.20 +0.6 35,633 - Pf1N1B4_5281 0.20 +1.4 35,633 - Pf1N1B4_5281 0.20 -0.4 35,633 - Pf1N1B4_5281 0.20 +0.7 35,700 + Pf1N1B4_5281 0.30 +0.6 35,700 + Pf1N1B4_5281 0.30 -1.6 35,701 - -1.0 35,701 - Pf1N1B4_5281 0.31 +1.6 35,701 - Pf1N1B4_5281 0.31 +0.4 35,701 - Pf1N1B4_5281 0.31 -0.4 35,701 - Pf1N1B4_5281 0.31 +1.0 35,701 - Pf1N1B4_5281 0.31 +0.6 35,715 + Pf1N1B4_5281 0.33 -1.0 35,715 + Pf1N1B4_5281 0.33 -0.0 35,715 + +0.4 35,716 - Pf1N1B4_5281 0.33 -0.0 35,716 - Pf1N1B4_5281 0.33 -0.6 35,747 + Pf1N1B4_5281 0.38 -2.3 35,747 + Pf1N1B4_5281 0.38 -0.7 35,747 + Pf1N1B4_5281 0.38 -0.3 35,747 + Pf1N1B4_5281 0.38 +0.4 35,747 + Pf1N1B4_5281 0.38 -0.0 35,747 + Pf1N1B4_5281 0.38 -2.8 35,748 - Pf1N1B4_5281 0.38 +0.7 35,748 - Pf1N1B4_5281 0.38 +0.6 35,748 - Pf1N1B4_5281 0.38 -0.6 35,748 - -0.0 35,748 - Pf1N1B4_5281 0.38 +0.4 35,748 - Pf1N1B4_5281 0.38 -0.4 35,748 - Pf1N1B4_5281 0.38 -0.6 35,748 - Pf1N1B4_5281 0.38 -0.3 35,748 - Pf1N1B4_5281 0.38 -0.0 35,748 - Pf1N1B4_5281 0.38 -0.5 35,793 - Pf1N1B4_5281 0.45 -0.7 35,857 + Pf1N1B4_5281 0.55 -1.0 35,858 - Pf1N1B4_5281 0.55 -0.0 35,858 - Pf1N1B4_5281 0.55 +0.7 35,897 + Pf1N1B4_5281 0.61 -0.4 35,897 + Pf1N1B4_5281 0.61 +1.3 35,897 + Pf1N1B4_5281 0.61 +0.2 35,898 - Pf1N1B4_5281 0.61 -1.0 35,898 - Pf1N1B4_5281 0.61 +1.0 36,150 + -1.6 36,151 - -0.2 36,151 - -0.0 36,171 + -0.0 36,171 + +1.8 36,223 + +0.9 36,223 + -0.0 36,223 + -0.6 36,223 + -1.0 36,223 + -0.0 36,224 - -0.0 36,224 - -0.2 36,224 - -1.6 36,224 - -1.6 36,224 - -1.6 36,224 - -0.2 36,224 - -1.0 36,224 - +0.7 36,224 - -1.0 36,341 + +0.4 36,341 + -0.0 36,341 + -1.3 36,341 + -0.8 36,341 + +0.4 36,341 + +1.2 36,341 + +2.0 36,342 - -0.4 36,342 - -0.2 36,342 - +0.9 36,342 - -0.0 36,389 - Pf1N1B4_5282 0.15 +1.6 36,417 + Pf1N1B4_5282 0.32 +1.8 36,417 + -0.0 36,417 + Pf1N1B4_5282 0.32 -0.0 36,417 + Pf1N1B4_5282 0.32 -0.7 36,446 + Pf1N1B4_5282 0.49 -0.5 36,446 + Pf1N1B4_5282 0.49 -0.0 36,446 + Pf1N1B4_5282 0.49 +0.6 36,447 - Pf1N1B4_5282 0.49 -0.0 36,447 - Pf1N1B4_5282 0.49 +2.3 36,531 + +1.0 36,633 - -1.0 36,635 + Pf1N1B4_5283 0.15 -0.0 36,635 + Pf1N1B4_5283 0.15 +0.8 36,635 + Pf1N1B4_5283 0.15 +0.8 36,635 + Pf1N1B4_5283 0.15 +1.2 36,890 - Pf1N1B4_5283 0.85 +1.6 36,941 + +1.0 36,941 + -0.2 36,942 - -0.0 36,942 - -0.0 36,960 + +0.6 36,961 - -1.6 37,001 + -0.0 37,001 + -0.3 37,001 + -0.0 37,052 - +1.0 37,079 + -1.0 37,080 - -0.0 37,080 - -0.3 37,170 + -0.8 37,171 - -1.0 37,198 + -0.6 37,199 - +1.0 37,201 + -0.4 37,201 + +0.6 37,201 + -0.9 37,202 - -0.0 37,202 - -0.7 37,202 - +0.7 37,202 - -1.0 37,248 - +0.7 37,295 + Pf1N1B4_5284 0.14 -0.5 37,316 + Pf1N1B4_5284 0.26 +1.0 37,356 + Pf1N1B4_5284 0.50 -0.0 37,356 + Pf1N1B4_5284 0.50 -0.0 37,357 - Pf1N1B4_5284 0.50 -1.0 37,368 + Pf1N1B4_5284 0.57 -0.0 37,369 - -0.0 37,369 - Pf1N1B4_5284 0.57 -0.6 37,476 - -0.6 37,484 - -0.0 37,519 - +1.7 37,535 + -0.0 37,536 - +0.7 37,552 + -0.1 37,599 + +0.3 37,599 + -0.2 37,599 + -1.0 37,600 - +1.3 37,600 - +1.8 37,686 + +0.4 37,687 - -0.0 37,714 - -0.3 37,765 + -0.6 37,766 - +1.8 37,766 - +0.3 37,796 + +0.3 37,796 + -0.7 37,797 - +1.6 37,817 + +0.1 37,817 + -1.2 37,818 - -1.3 37,821 + -0.0 37,822 - +0.3 37,822 - +3.0 37,837 + +1.0 37,837 + -0.0 37,837 + +1.8 37,837 + -1.6 37,838 - -1.3 37,878 + +0.7 37,878 + -0.7 37,878 + -1.6 37,878 + +0.6 37,878 + -0.6 37,879 - +0.6 37,883 + -0.0 37,895 + -1.0 37,896 - +0.3 37,901 + +1.0 37,901 + -1.8 37,902 - -0.0 37,903 + +1.0 37,903 + -0.3 37,903 + +0.7 37,903 + -1.0 37,903 + +0.6 37,903 + +1.6 37,903 + -1.0 37,903 + +0.1 37,903 + +0.9 37,903 + +0.5 37,903 + +0.3 37,903 + +0.6 37,903 + -0.5 37,903 + -1.0 37,903 + +0.6 37,903 + +1.9 37,904 - -1.0 37,904 - +0.4 37,904 - +1.2 37,904 - +1.4 37,904 - +1.6 37,904 - +1.0 37,938 + +1.0 37,938 + +0.4 37,938 + -1.3 37,938 + -1.6
Or see this region's nucleotide sequence