Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5260 and Pf1N1B4_5261 are separated by 280 nucleotides Pf1N1B4_5261 and Pf1N1B4_5262 are separated by 372 nucleotides
Pf1N1B4_5260 - hypothetical protein, at 19,541 to 20,695
_5260
Pf1N1B4_5261 - hypothetical protein, at 20,976 to 21,173
_5261
Pf1N1B4_5262 - hypothetical protein, at 21,546 to 22,034
_5262
Position (kb) 20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 20.274 kb on - strand, within Pf1N1B4_5260 at 20.277 kb on + strand at 20.281 kb on + strand, within Pf1N1B4_5260 at 20.285 kb on - strand, within Pf1N1B4_5260 at 20.627 kb on + strand at 20.627 kb on + strand at 20.630 kb on + strand at 20.657 kb on + strand at 20.758 kb on + strand at 20.977 kb on + strand at 20.977 kb on + strand at 21.099 kb on + strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.200 kb on + strand at 21.220 kb on + strand at 21.359 kb on + strand at 21.360 kb on - strand at 21.589 kb on + strand at 21.724 kb on + strand, within Pf1N1B4_5262 at 21.725 kb on - strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.943 kb on + strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.961 kb on + strand, within Pf1N1B4_5262 at 22.078 kb on - strand at 22.104 kb on + strand at 22.105 kb on - strand at 22.105 kb on - strand at 22.159 kb on + strand at 22.159 kb on + strand at 22.160 kb on - strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 20,274 - Pf1N1B4_5260 0.63 -1.6 20,277 + -0.0 20,281 + Pf1N1B4_5260 0.64 +3.2 20,285 - Pf1N1B4_5260 0.64 +1.0 20,627 + -0.0 20,627 + +0.6 20,630 + -0.7 20,657 + -0.4 20,758 + -0.0 20,977 + -1.8 20,977 + +0.1 21,099 + Pf1N1B4_5261 0.62 -0.4 21,100 - Pf1N1B4_5261 0.63 -0.2 21,100 - Pf1N1B4_5261 0.63 -0.4 21,100 - Pf1N1B4_5261 0.63 -3.0 21,200 + -1.2 21,220 + -1.0 21,359 + -1.0 21,360 - +0.3 21,589 + -0.7 21,724 + Pf1N1B4_5262 0.36 -0.3 21,725 - Pf1N1B4_5262 0.37 +0.2 21,904 + Pf1N1B4_5262 0.73 -0.5 21,904 + Pf1N1B4_5262 0.73 -0.6 21,904 + Pf1N1B4_5262 0.73 -1.3 21,905 - Pf1N1B4_5262 0.73 +0.6 21,905 - Pf1N1B4_5262 0.73 -1.6 21,905 - Pf1N1B4_5262 0.73 -0.6 21,905 - Pf1N1B4_5262 0.73 -1.0 21,941 + Pf1N1B4_5262 0.81 +0.7 21,941 + Pf1N1B4_5262 0.81 -0.0 21,943 + Pf1N1B4_5262 0.81 -0.6 21,944 - Pf1N1B4_5262 0.81 +2.3 21,944 - Pf1N1B4_5262 0.81 -1.0 21,961 + Pf1N1B4_5262 0.85 +0.6 22,078 - -0.0 22,104 + +0.6 22,105 - +0.4 22,105 - -1.3 22,159 + -0.0 22,159 + +0.3 22,160 - +0.3 22,162 + +0.3 22,162 + -0.5 22,162 + +0.1 22,162 + -1.6 22,162 + +0.5
Or see this region's nucleotide sequence