Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4991 and Pf1N1B4_4992 are separated by 769 nucleotides Pf1N1B4_4992 and Pf1N1B4_4993 are separated by 140 nucleotides
Pf1N1B4_4991 - Transcriptional regulator, AraC family, at 5,537,349 to 5,537,834
_4991
Pf1N1B4_4992 - Dienelactone hydrolase and related enzymes, at 5,538,604 to 5,539,563
_4992
Pf1N1B4_4993 - Arabinose efflux permease, at 5,539,704 to 5,540,849
_4993
Position (kb)
5538
5539
5540 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5537.700 kb on + strand, within Pf1N1B4_4991 at 5537.701 kb on - strand at 5538.280 kb on + strand at 5538.302 kb on - strand at 5538.360 kb on - strand at 5538.360 kb on - strand at 5538.449 kb on + strand at 5538.450 kb on - strand at 5538.450 kb on - strand at 5538.515 kb on + strand at 5538.516 kb on - strand at 5538.677 kb on - strand at 5538.797 kb on - strand, within Pf1N1B4_4992 at 5538.901 kb on + strand, within Pf1N1B4_4992 at 5539.033 kb on - strand at 5539.061 kb on - strand, within Pf1N1B4_4992 at 5539.061 kb on - strand, within Pf1N1B4_4992 at 5539.061 kb on - strand at 5539.061 kb on - strand at 5539.238 kb on - strand at 5539.343 kb on - strand, within Pf1N1B4_4992 at 5539.343 kb on - strand, within Pf1N1B4_4992 at 5539.436 kb on - strand, within Pf1N1B4_4992 at 5539.436 kb on - strand, within Pf1N1B4_4992 at 5539.633 kb on - strand at 5539.636 kb on - strand at 5539.679 kb on + strand at 5539.679 kb on + strand at 5539.781 kb on - strand at 5539.914 kb on - strand, within Pf1N1B4_4993 at 5539.953 kb on + strand, within Pf1N1B4_4993 at 5539.954 kb on - strand, within Pf1N1B4_4993 at 5539.996 kb on + strand, within Pf1N1B4_4993 at 5540.198 kb on - strand, within Pf1N1B4_4993 at 5540.343 kb on + strand, within Pf1N1B4_4993
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 5,537,700 + Pf1N1B4_4991 0.72 -1.1 5,537,701 - -0.5 5,538,280 + +0.8 5,538,302 - -0.0 5,538,360 - +1.8 5,538,360 - +0.1 5,538,449 + -1.5 5,538,450 - +0.1 5,538,450 - -0.5 5,538,515 + -0.2 5,538,516 - -1.1 5,538,677 - -0.5 5,538,797 - Pf1N1B4_4992 0.20 -0.9 5,538,901 + Pf1N1B4_4992 0.31 -1.0 5,539,033 - -0.1 5,539,061 - Pf1N1B4_4992 0.48 -2.5 5,539,061 - Pf1N1B4_4992 0.48 +0.2 5,539,061 - +2.1 5,539,061 - -0.5 5,539,238 - +1.8 5,539,343 - Pf1N1B4_4992 0.77 -2.7 5,539,343 - Pf1N1B4_4992 0.77 +0.1 5,539,436 - Pf1N1B4_4992 0.87 -0.2 5,539,436 - Pf1N1B4_4992 0.87 -0.9 5,539,633 - -0.5 5,539,636 - +0.1 5,539,679 + -0.1 5,539,679 + -0.5 5,539,781 - +1.1 5,539,914 - Pf1N1B4_4993 0.18 -0.5 5,539,953 + Pf1N1B4_4993 0.22 +0.1 5,539,954 - Pf1N1B4_4993 0.22 +0.2 5,539,996 + Pf1N1B4_4993 0.25 -0.5 5,540,198 - Pf1N1B4_4993 0.43 -0.5 5,540,343 + Pf1N1B4_4993 0.56 -0.1
Or see this region's nucleotide sequence