Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4959

Experiment: Dimethyl Sulfoxide 6.5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4958 and Pf1N1B4_4959 are separated by 3 nucleotidesPf1N1B4_4959 and Pf1N1B4_4960 overlap by 4 nucleotidesPf1N1B4_4960 and Pf1N1B4_4961 are separated by 98 nucleotides Pf1N1B4_4958 - Protein containing domains DUF404, DUF407, at 5,498,657 to 5,499,988 _4958 Pf1N1B4_4959 - Protein containing domains DUF403, at 5,499,992 to 5,500,942 _4959 Pf1N1B4_4960 - Protein containing transglutaminase-like domain, putative cysteine protease, at 5,500,939 to 5,501,736 _4960 Pf1N1B4_4961 - FIG00441227: hypothetical protein, at 5,501,835 to 5,502,554 _4961 Position (kb) 5499 5500 5501Strain fitness (log2 ratio) -2 -1 0 1at 5499.039 kb on - strand, within Pf1N1B4_4958at 5499.041 kb on + strand, within Pf1N1B4_4958at 5499.042 kb on - strand, within Pf1N1B4_4958at 5499.042 kb on - strand, within Pf1N1B4_4958at 5499.084 kb on - strand, within Pf1N1B4_4958at 5499.084 kb on - strand, within Pf1N1B4_4958at 5499.084 kb on - strand, within Pf1N1B4_4958at 5499.257 kb on + strand, within Pf1N1B4_4958at 5499.258 kb on - strandat 5499.258 kb on - strand, within Pf1N1B4_4958at 5499.258 kb on - strandat 5499.341 kb on + strand, within Pf1N1B4_4958at 5499.341 kb on + strand, within Pf1N1B4_4958at 5499.341 kb on + strandat 5499.341 kb on + strand, within Pf1N1B4_4958at 5499.341 kb on + strandat 5499.341 kb on + strand, within Pf1N1B4_4958at 5499.342 kb on - strandat 5499.342 kb on - strand, within Pf1N1B4_4958at 5499.533 kb on + strandat 5499.534 kb on - strand, within Pf1N1B4_4958at 5499.563 kb on + strand, within Pf1N1B4_4958at 5499.564 kb on - strand, within Pf1N1B4_4958at 5499.564 kb on - strand, within Pf1N1B4_4958at 5499.770 kb on + strand, within Pf1N1B4_4958at 5499.771 kb on - strand, within Pf1N1B4_4958at 5499.771 kb on - strand, within Pf1N1B4_4958at 5499.771 kb on - strand, within Pf1N1B4_4958at 5499.842 kb on - strand, within Pf1N1B4_4958at 5499.861 kb on + strandat 5499.986 kb on + strandat 5499.987 kb on - strandat 5499.987 kb on - strandat 5499.987 kb on - strandat 5500.170 kb on - strand, within Pf1N1B4_4959at 5500.250 kb on + strand, within Pf1N1B4_4959at 5500.250 kb on + strand, within Pf1N1B4_4959at 5500.652 kb on + strand, within Pf1N1B4_4959at 5500.652 kb on + strand, within Pf1N1B4_4959at 5500.652 kb on + strand, within Pf1N1B4_4959at 5500.653 kb on - strand, within Pf1N1B4_4959at 5500.925 kb on + strandat 5500.925 kb on + strandat 5500.938 kb on + strandat 5500.938 kb on + strandat 5500.969 kb on + strandat 5500.969 kb on + strandat 5500.970 kb on - strandat 5500.970 kb on - strandat 5500.970 kb on - strandat 5501.353 kb on + strand, within Pf1N1B4_4960at 5501.479 kb on + strand, within Pf1N1B4_4960at 5501.479 kb on + strandat 5501.480 kb on - strand, within Pf1N1B4_4960at 5501.480 kb on - strand, within Pf1N1B4_4960at 5501.563 kb on + strand, within Pf1N1B4_4960at 5501.564 kb on - strand, within Pf1N1B4_4960at 5501.824 kb on - strandat 5501.824 kb on - strandat 5501.824 kb on - strandat 5501.835 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol%
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5,499,039 - Pf1N1B4_4958 0.29 -0.1
5,499,041 + Pf1N1B4_4958 0.29 +0.1
5,499,042 - Pf1N1B4_4958 0.29 -0.5
5,499,042 - Pf1N1B4_4958 0.29 -2.3
5,499,084 - Pf1N1B4_4958 0.32 -0.9
5,499,084 - Pf1N1B4_4958 0.32 +0.5
5,499,084 - Pf1N1B4_4958 0.32 +1.1
5,499,257 + Pf1N1B4_4958 0.45 +0.1
5,499,258 - -1.5
5,499,258 - Pf1N1B4_4958 0.45 -0.5
5,499,258 - +1.1
5,499,341 + Pf1N1B4_4958 0.51 -0.5
5,499,341 + Pf1N1B4_4958 0.51 -1.8
5,499,341 + -0.5
5,499,341 + Pf1N1B4_4958 0.51 -0.5
5,499,341 + -0.5
5,499,341 + Pf1N1B4_4958 0.51 -2.1
5,499,342 - +0.1
5,499,342 - Pf1N1B4_4958 0.51 -0.3
5,499,533 + -0.5
5,499,534 - Pf1N1B4_4958 0.66 -0.1
5,499,563 + Pf1N1B4_4958 0.68 -0.5
5,499,564 - Pf1N1B4_4958 0.68 -0.8
5,499,564 - Pf1N1B4_4958 0.68 -0.7
5,499,770 + Pf1N1B4_4958 0.84 -0.2
5,499,771 - Pf1N1B4_4958 0.84 -1.1
5,499,771 - Pf1N1B4_4958 0.84 +0.1
5,499,771 - Pf1N1B4_4958 0.84 -0.0
5,499,842 - Pf1N1B4_4958 0.89 +0.5
5,499,861 + -0.5
5,499,986 + -1.5
5,499,987 - -0.9
5,499,987 - -1.8
5,499,987 - +1.1
5,500,170 - Pf1N1B4_4959 0.19 -1.0
5,500,250 + Pf1N1B4_4959 0.27 -0.8
5,500,250 + Pf1N1B4_4959 0.27 -1.5
5,500,652 + Pf1N1B4_4959 0.69 +0.1
5,500,652 + Pf1N1B4_4959 0.69 +1.1
5,500,652 + Pf1N1B4_4959 0.69 +0.1
5,500,653 - Pf1N1B4_4959 0.70 -0.5
5,500,925 + +0.5
5,500,925 + -1.3
5,500,938 + +0.3
5,500,938 + +0.3
5,500,969 + +1.1
5,500,969 + +1.5
5,500,970 - -1.5
5,500,970 - +0.1
5,500,970 - +0.1
5,501,353 + Pf1N1B4_4960 0.52 -0.5
5,501,479 + Pf1N1B4_4960 0.68 -0.5
5,501,479 + +0.5
5,501,480 - Pf1N1B4_4960 0.68 +1.5
5,501,480 - Pf1N1B4_4960 0.68 +0.7
5,501,563 + Pf1N1B4_4960 0.78 -0.9
5,501,564 - Pf1N1B4_4960 0.78 -0.5
5,501,824 - +0.5
5,501,824 - +0.8
5,501,824 - -0.5
5,501,835 - +1.4

Or see this region's nucleotide sequence