Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4896 and Pf1N1B4_4897 are separated by 224 nucleotides Pf1N1B4_4897 and Pf1N1B4_4898 are separated by 99 nucleotides Pf1N1B4_4898 and Pf1N1B4_4899 are separated by 654 nucleotides
Pf1N1B4_4896 - N-ethylmaleimide reductase, at 5,418,216 to 5,419,238
_4896
Pf1N1B4_4897 - Transcriptional regulator, LysR family, at 5,419,463 to 5,420,347
_4897
Pf1N1B4_4898 - Alcohol dehydrogenase (EC 1.1.1.1), at 5,420,447 to 5,420,560
_4898
Pf1N1B4_4899 - Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog, at 5,421,215 to 5,422,672
_4899
Position (kb)
5419
5420
5421 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5418.510 kb on + strand, within Pf1N1B4_4896 at 5418.539 kb on - strand, within Pf1N1B4_4896 at 5418.558 kb on + strand, within Pf1N1B4_4896 at 5418.608 kb on + strand, within Pf1N1B4_4896 at 5418.681 kb on + strand, within Pf1N1B4_4896 at 5418.682 kb on - strand, within Pf1N1B4_4896 at 5418.727 kb on + strand, within Pf1N1B4_4896 at 5418.728 kb on - strand, within Pf1N1B4_4896 at 5418.728 kb on - strand, within Pf1N1B4_4896 at 5418.738 kb on - strand, within Pf1N1B4_4896 at 5418.984 kb on + strand at 5419.016 kb on - strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.097 kb on - strand, within Pf1N1B4_4896 at 5419.097 kb on - strand, within Pf1N1B4_4896 at 5419.097 kb on - strand at 5419.179 kb on - strand at 5419.179 kb on - strand at 5419.179 kb on - strand at 5419.189 kb on + strand at 5419.190 kb on - strand at 5419.192 kb on + strand at 5419.287 kb on + strand at 5419.288 kb on - strand at 5419.301 kb on + strand at 5419.329 kb on + strand at 5419.330 kb on - strand at 5419.330 kb on - strand at 5419.354 kb on + strand at 5419.361 kb on - strand at 5419.441 kb on + strand at 5419.600 kb on - strand, within Pf1N1B4_4897 at 5419.600 kb on - strand at 5419.762 kb on - strand at 5420.145 kb on - strand at 5420.145 kb on - strand, within Pf1N1B4_4897 at 5420.240 kb on + strand at 5420.241 kb on - strand, within Pf1N1B4_4897 at 5420.241 kb on - strand, within Pf1N1B4_4897 at 5420.321 kb on + strand at 5420.321 kb on + strand at 5420.322 kb on - strand at 5420.495 kb on + strand at 5420.496 kb on - strand, within Pf1N1B4_4898 at 5420.496 kb on - strand, within Pf1N1B4_4898 at 5420.496 kb on - strand, within Pf1N1B4_4898 at 5420.496 kb on - strand, within Pf1N1B4_4898 at 5420.562 kb on - strand at 5420.562 kb on - strand at 5420.562 kb on - strand at 5420.610 kb on - strand at 5420.610 kb on - strand at 5420.610 kb on - strand at 5420.610 kb on - strand at 5420.610 kb on - strand at 5420.614 kb on - strand at 5420.615 kb on + strand at 5420.665 kb on - strand at 5420.694 kb on - strand at 5420.911 kb on - strand at 5421.019 kb on + strand at 5421.021 kb on + strand at 5421.299 kb on + strand at 5421.329 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 5,418,510 + Pf1N1B4_4896 0.29 +0.5 5,418,539 - Pf1N1B4_4896 0.32 -2.1 5,418,558 + Pf1N1B4_4896 0.33 +2.1 5,418,608 + Pf1N1B4_4896 0.38 -0.5 5,418,681 + Pf1N1B4_4896 0.45 -1.5 5,418,682 - Pf1N1B4_4896 0.46 -1.5 5,418,727 + Pf1N1B4_4896 0.50 +0.8 5,418,728 - Pf1N1B4_4896 0.50 +0.1 5,418,728 - Pf1N1B4_4896 0.50 -0.8 5,418,738 - Pf1N1B4_4896 0.51 +1.3 5,418,984 + -0.5 5,419,016 - Pf1N1B4_4896 0.78 +2.1 5,419,096 + Pf1N1B4_4896 0.86 +1.1 5,419,096 + Pf1N1B4_4896 0.86 +0.1 5,419,096 + Pf1N1B4_4896 0.86 -1.2 5,419,097 - Pf1N1B4_4896 0.86 +0.1 5,419,097 - Pf1N1B4_4896 0.86 -0.7 5,419,097 - -0.5 5,419,179 - +0.1 5,419,179 - +0.5 5,419,179 - +1.1 5,419,189 + +0.5 5,419,190 - -0.5 5,419,192 + +0.1 5,419,287 + +0.2 5,419,288 - +1.3 5,419,301 + -0.5 5,419,329 + -1.5 5,419,330 - +1.5 5,419,330 - +1.3 5,419,354 + -0.5 5,419,361 - -0.5 5,419,441 + +1.1 5,419,600 - Pf1N1B4_4897 0.15 -0.9 5,419,600 - -2.1 5,419,762 - -1.5 5,420,145 - -1.1 5,420,145 - Pf1N1B4_4897 0.77 -1.2 5,420,240 + +0.1 5,420,241 - Pf1N1B4_4897 0.88 -0.3 5,420,241 - Pf1N1B4_4897 0.88 -1.1 5,420,321 + -0.5 5,420,321 + -0.8 5,420,322 - -0.3 5,420,495 + -2.5 5,420,496 - Pf1N1B4_4898 0.43 +1.1 5,420,496 - Pf1N1B4_4898 0.43 +0.8 5,420,496 - Pf1N1B4_4898 0.43 +0.1 5,420,496 - Pf1N1B4_4898 0.43 -0.2 5,420,562 - -0.2 5,420,562 - +0.0 5,420,562 - +0.1 5,420,610 - +1.1 5,420,610 - -0.5 5,420,610 - -2.5 5,420,610 - -1.1 5,420,610 - -1.1 5,420,614 - -0.5 5,420,615 + +3.0 5,420,665 - -2.1 5,420,694 - -1.5 5,420,911 - +0.1 5,421,019 + -1.5 5,421,021 + -1.5 5,421,299 + +1.4 5,421,329 + -1.0
Or see this region's nucleotide sequence