Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4584 and Pf1N1B4_4585 are separated by 1 nucleotides Pf1N1B4_4585 and Pf1N1B4_4586 are separated by 73 nucleotides Pf1N1B4_4586 and Pf1N1B4_4587 are separated by 35 nucleotides
Pf1N1B4_4584 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 5,054,442 to 5,056,610
_4584
Pf1N1B4_4585 - Putative Isoquinoline 1-oxidoreductase subunit, Mll3835 protein, at 5,056,612 to 5,057,190
_4585
Pf1N1B4_4586 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 5,057,264 to 5,057,872
_4586
Pf1N1B4_4587 - probable dioxygenase, at 5,057,908 to 5,058,333
_4587
Position (kb)
5056
5057
5058 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5055.682 kb on - strand, within Pf1N1B4_4584 at 5055.927 kb on + strand, within Pf1N1B4_4584 at 5055.928 kb on - strand, within Pf1N1B4_4584 at 5055.928 kb on - strand, within Pf1N1B4_4584 at 5056.221 kb on + strand, within Pf1N1B4_4584 at 5056.221 kb on + strand at 5056.221 kb on + strand, within Pf1N1B4_4584 at 5056.222 kb on - strand, within Pf1N1B4_4584 at 5056.367 kb on + strand, within Pf1N1B4_4584 at 5056.420 kb on - strand at 5056.758 kb on + strand, within Pf1N1B4_4585 at 5056.860 kb on + strand, within Pf1N1B4_4585 at 5056.861 kb on - strand, within Pf1N1B4_4585 at 5057.185 kb on - strand at 5057.236 kb on - strand at 5057.236 kb on - strand at 5057.759 kb on + strand, within Pf1N1B4_4586 at 5057.842 kb on + strand at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.127 kb on - strand, within Pf1N1B4_4587
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 5,055,682 - Pf1N1B4_4584 0.57 -0.8 5,055,927 + Pf1N1B4_4584 0.68 +1.6 5,055,928 - Pf1N1B4_4584 0.69 -0.6 5,055,928 - Pf1N1B4_4584 0.69 +0.6 5,056,221 + Pf1N1B4_4584 0.82 -0.0 5,056,221 + -1.5 5,056,221 + Pf1N1B4_4584 0.82 -1.8 5,056,222 - Pf1N1B4_4584 0.82 +1.5 5,056,367 + Pf1N1B4_4584 0.89 -0.0 5,056,420 - +1.5 5,056,758 + Pf1N1B4_4585 0.25 +0.3 5,056,860 + Pf1N1B4_4585 0.43 -0.3 5,056,861 - Pf1N1B4_4585 0.43 -2.8 5,057,185 - -0.5 5,057,236 - +0.5 5,057,236 - -0.9 5,057,759 + Pf1N1B4_4586 0.81 -2.0 5,057,842 + +0.9 5,058,126 + Pf1N1B4_4587 0.51 +0.1 5,058,126 + Pf1N1B4_4587 0.51 +0.5 5,058,126 + Pf1N1B4_4587 0.51 -0.9 5,058,127 - Pf1N1B4_4587 0.51 +0.2
Or see this region's nucleotide sequence