Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4548 and Pf1N1B4_4549 are separated by 58 nucleotides Pf1N1B4_4549 and Pf1N1B4_4550 are separated by 1 nucleotides
Pf1N1B4_4548 - Acyl carrier protein, putative, at 5,016,496 to 5,017,068
_4548
Pf1N1B4_4549 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41), at 5,017,127 to 5,018,347
_4549
Pf1N1B4_4550 - beta-ketoacyl synthase, putative, at 5,018,349 to 5,019,431
_4550
Position (kb)
5017
5018
5019 Strain fitness (log2 ratio)
-1
0
1 at 5016.283 kb on - strand at 5016.407 kb on + strand at 5016.408 kb on - strand at 5016.457 kb on + strand at 5016.460 kb on + strand at 5016.461 kb on - strand at 5016.461 kb on - strand at 5016.464 kb on + strand at 5016.465 kb on - strand at 5016.465 kb on - strand at 5016.494 kb on - strand at 5016.528 kb on + strand at 5016.594 kb on + strand, within Pf1N1B4_4548 at 5016.594 kb on + strand, within Pf1N1B4_4548 at 5016.667 kb on + strand, within Pf1N1B4_4548 at 5016.667 kb on + strand, within Pf1N1B4_4548 at 5017.097 kb on + strand at 5017.098 kb on - strand at 5017.098 kb on - strand at 5017.236 kb on - strand at 5017.403 kb on - strand, within Pf1N1B4_4549 at 5017.703 kb on + strand at 5018.073 kb on + strand, within Pf1N1B4_4549 at 5018.074 kb on - strand, within Pf1N1B4_4549 at 5018.213 kb on + strand at 5018.214 kb on - strand at 5018.355 kb on - strand at 5018.363 kb on + strand at 5018.572 kb on - strand, within Pf1N1B4_4550 at 5018.657 kb on - strand, within Pf1N1B4_4550 at 5018.716 kb on + strand, within Pf1N1B4_4550 at 5018.738 kb on + strand, within Pf1N1B4_4550 at 5018.739 kb on - strand, within Pf1N1B4_4550 at 5018.930 kb on + strand, within Pf1N1B4_4550 at 5018.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 5,016,283 - +0.4 5,016,407 + -0.5 5,016,408 - -0.5 5,016,457 + +1.1 5,016,460 + +1.5 5,016,461 - +0.0 5,016,461 - -0.6 5,016,464 + +0.4 5,016,465 - +0.8 5,016,465 - -0.0 5,016,494 - -1.0 5,016,528 + -1.5 5,016,594 + Pf1N1B4_4548 0.17 -0.5 5,016,594 + Pf1N1B4_4548 0.17 +0.1 5,016,667 + Pf1N1B4_4548 0.30 -0.5 5,016,667 + Pf1N1B4_4548 0.30 -0.5 5,017,097 + -0.5 5,017,098 - +1.0 5,017,098 - +0.8 5,017,236 - -0.5 5,017,403 - Pf1N1B4_4549 0.23 -0.3 5,017,703 + +0.3 5,018,073 + Pf1N1B4_4549 0.77 -0.9 5,018,074 - Pf1N1B4_4549 0.78 -0.8 5,018,213 + -1.5 5,018,214 - -0.5 5,018,355 - -0.5 5,018,363 + +0.8 5,018,572 - Pf1N1B4_4550 0.21 -1.2 5,018,657 - Pf1N1B4_4550 0.28 -1.0 5,018,716 + Pf1N1B4_4550 0.34 +0.7 5,018,738 + Pf1N1B4_4550 0.36 +0.5 5,018,739 - Pf1N1B4_4550 0.36 -0.5 5,018,930 + Pf1N1B4_4550 0.54 -0.2 5,018,950 - -0.5
Or see this region's nucleotide sequence