Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4433 and Pf1N1B4_4434 are separated by 18 nucleotides Pf1N1B4_4434 and Pf1N1B4_4435 overlap by 4 nucleotides Pf1N1B4_4435 and Pf1N1B4_4436 are separated by 229 nucleotides Pf1N1B4_4436 and Pf1N1B4_4437 are separated by 74 nucleotides
Pf1N1B4_4433 - FIG035246: DoxX family protein, at 4,894,669 to 4,895,184
_4433
Pf1N1B4_4434 - hypothetical protein, at 4,895,203 to 4,895,325
_4434
Pf1N1B4_4435 - Inner membrane protein, at 4,895,322 to 4,895,951
_4435
Pf1N1B4_4436 - Glutaminase (EC 3.5.1.2), at 4,896,181 to 4,897,089
_4436
Pf1N1B4_4437 - hypothetical protein, at 4,897,164 to 4,898,282
_4437
Position (kb)
4896
4897
4898 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4895.429 kb on - strand at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.653 kb on + strand, within Pf1N1B4_4435 at 4895.659 kb on + strand, within Pf1N1B4_4435 at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4895.660 kb on - strand at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4896.087 kb on + strand at 4896.087 kb on + strand at 4896.250 kb on + strand at 4896.250 kb on + strand at 4896.251 kb on - strand at 4896.302 kb on - strand, within Pf1N1B4_4436 at 4896.646 kb on + strand, within Pf1N1B4_4436 at 4896.736 kb on + strand, within Pf1N1B4_4436 at 4896.892 kb on + strand at 4896.892 kb on + strand, within Pf1N1B4_4436 at 4897.437 kb on + strand, within Pf1N1B4_4437 at 4897.455 kb on + strand, within Pf1N1B4_4437 at 4897.712 kb on + strand, within Pf1N1B4_4437 at 4897.712 kb on + strand, within Pf1N1B4_4437 at 4897.960 kb on + strand, within Pf1N1B4_4437 at 4897.960 kb on + strand, within Pf1N1B4_4437 at 4897.960 kb on + strand, within Pf1N1B4_4437 at 4897.961 kb on - strand, within Pf1N1B4_4437 at 4897.961 kb on - strand, within Pf1N1B4_4437
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 4,895,429 - +0.5 4,895,429 - Pf1N1B4_4435 0.17 +2.3 4,895,429 - Pf1N1B4_4435 0.17 -0.5 4,895,429 - Pf1N1B4_4435 0.17 +0.1 4,895,429 - Pf1N1B4_4435 0.17 +2.3 4,895,653 + Pf1N1B4_4435 0.53 +0.3 4,895,659 + Pf1N1B4_4435 0.53 -2.5 4,895,660 - Pf1N1B4_4435 0.54 +0.0 4,895,660 - -0.5 4,895,660 - Pf1N1B4_4435 0.54 +1.1 4,895,660 - Pf1N1B4_4435 0.54 +0.5 4,896,087 + -0.5 4,896,087 + +1.1 4,896,250 + +1.7 4,896,250 + -0.5 4,896,251 - +0.3 4,896,302 - Pf1N1B4_4436 0.13 -1.3 4,896,646 + Pf1N1B4_4436 0.51 -0.2 4,896,736 + Pf1N1B4_4436 0.61 -0.5 4,896,892 + +0.5 4,896,892 + Pf1N1B4_4436 0.78 -0.5 4,897,437 + Pf1N1B4_4437 0.24 -1.5 4,897,455 + Pf1N1B4_4437 0.26 -1.5 4,897,712 + Pf1N1B4_4437 0.49 +0.5 4,897,712 + Pf1N1B4_4437 0.49 -2.1 4,897,960 + Pf1N1B4_4437 0.71 -0.7 4,897,960 + Pf1N1B4_4437 0.71 -0.5 4,897,960 + Pf1N1B4_4437 0.71 -0.9 4,897,961 - Pf1N1B4_4437 0.71 -1.5 4,897,961 - Pf1N1B4_4437 0.71 -0.7
Or see this region's nucleotide sequence