Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4328 and Pf1N1B4_4329 are separated by 267 nucleotides Pf1N1B4_4329 and Pf1N1B4_4330 are separated by 21 nucleotides Pf1N1B4_4330 and Pf1N1B4_4331 are separated by 28 nucleotides Pf1N1B4_4331 and Pf1N1B4_4332 are separated by 188 nucleotides
Pf1N1B4_4328 - Agmatine deiminase (EC 3.5.3.12), at 4,788,812 to 4,789,936
_4328
Pf1N1B4_4329 - Acyl dehydratase, at 4,790,204 to 4,790,689
_4329
Pf1N1B4_4330 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 4,790,711 to 4,791,553
_4330
Pf1N1B4_4331 - Transporter, LysE family, at 4,791,582 to 4,792,199
_4331
Pf1N1B4_4332 - Transcriptional regulator, AsnC family, at 4,792,388 to 4,792,840
_4332
Position (kb)
4790
4791
4792 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4789.755 kb on - strand, within Pf1N1B4_4328 at 4789.810 kb on + strand, within Pf1N1B4_4328 at 4789.811 kb on - strand, within Pf1N1B4_4328 at 4789.860 kb on + strand at 4789.861 kb on - strand at 4790.121 kb on - strand at 4790.210 kb on - strand at 4790.527 kb on + strand, within Pf1N1B4_4329 at 4790.575 kb on - strand, within Pf1N1B4_4329 at 4790.605 kb on + strand, within Pf1N1B4_4329 at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.867 kb on + strand, within Pf1N1B4_4330 at 4790.909 kb on - strand, within Pf1N1B4_4330 at 4791.423 kb on + strand, within Pf1N1B4_4330 at 4791.423 kb on + strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.554 kb on + strand at 4791.575 kb on + strand at 4791.575 kb on + strand at 4791.715 kb on + strand, within Pf1N1B4_4331 at 4791.759 kb on - strand, within Pf1N1B4_4331 at 4791.831 kb on + strand, within Pf1N1B4_4331 at 4791.831 kb on + strand, within Pf1N1B4_4331 at 4791.832 kb on - strand, within Pf1N1B4_4331 at 4792.053 kb on + strand at 4792.147 kb on - strand at 4792.148 kb on + strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.199 kb on + strand at 4792.200 kb on - strand at 4792.214 kb on + strand at 4792.214 kb on + strand at 4792.215 kb on - strand at 4792.215 kb on - strand at 4792.240 kb on + strand at 4792.300 kb on - strand at 4792.318 kb on - strand at 4792.348 kb on + strand at 4792.474 kb on + strand, within Pf1N1B4_4332
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 4,789,755 - Pf1N1B4_4328 0.84 -0.4 4,789,810 + Pf1N1B4_4328 0.89 -0.9 4,789,811 - Pf1N1B4_4328 0.89 -0.4 4,789,860 + -1.4 4,789,861 - +1.1 4,790,121 - -1.8 4,790,210 - -1.4 4,790,527 + Pf1N1B4_4329 0.66 -1.0 4,790,575 - Pf1N1B4_4329 0.76 -0.0 4,790,605 + Pf1N1B4_4329 0.83 +0.1 4,790,641 + +0.8 4,790,641 + -1.4 4,790,641 + +1.1 4,790,641 + -0.4 4,790,867 + Pf1N1B4_4330 0.19 -0.2 4,790,909 - Pf1N1B4_4330 0.23 +0.6 4,791,423 + Pf1N1B4_4330 0.84 -1.2 4,791,423 + Pf1N1B4_4330 0.84 -1.4 4,791,424 - Pf1N1B4_4330 0.85 -0.4 4,791,424 - Pf1N1B4_4330 0.85 -0.2 4,791,424 - Pf1N1B4_4330 0.85 -2.0 4,791,424 - Pf1N1B4_4330 0.85 -0.8 4,791,424 - -1.4 4,791,424 - Pf1N1B4_4330 0.85 -1.4 4,791,424 - Pf1N1B4_4330 0.85 -1.2 4,791,554 + -0.4 4,791,575 + +0.6 4,791,575 + -1.0 4,791,715 + Pf1N1B4_4331 0.22 -0.4 4,791,759 - Pf1N1B4_4331 0.29 -0.4 4,791,831 + Pf1N1B4_4331 0.40 +0.6 4,791,831 + Pf1N1B4_4331 0.40 +0.6 4,791,832 - Pf1N1B4_4331 0.40 -2.0 4,792,053 + -0.4 4,792,147 - -1.4 4,792,148 + -1.4 4,792,149 - +0.8 4,792,149 - +0.6 4,792,149 - -1.0 4,792,149 - +0.9 4,792,199 + -0.9 4,792,200 - -0.4 4,792,214 + -0.9 4,792,214 + +1.9 4,792,215 - +1.8 4,792,215 - -1.4 4,792,240 + -0.2 4,792,300 - -0.1 4,792,318 - -1.0 4,792,348 + +0.3 4,792,474 + Pf1N1B4_4332 0.19 -2.4
Or see this region's nucleotide sequence