Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4110 and Pf1N1B4_4111 are separated by 173 nucleotides Pf1N1B4_4111 and Pf1N1B4_4112 are separated by 141 nucleotides Pf1N1B4_4112 and Pf1N1B4_4113 overlap by 4 nucleotides Pf1N1B4_4113 and Pf1N1B4_4114 overlap by 2 nucleotides Pf1N1B4_4114 and Pf1N1B4_4115 are separated by 97 nucleotides
Pf1N1B4_4110 - hypothetical protein, at 4,556,372 to 4,556,707
_4110
Pf1N1B4_4111 - Panthothenate synthetase, at 4,556,881 to 4,557,171
_4111
Pf1N1B4_4112 - hypothetical protein, at 4,557,313 to 4,557,564
_4112
Pf1N1B4_4113 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 4,557,561 to 4,558,040
_4113
Pf1N1B4_4114 - hypothetical protein, at 4,558,039 to 4,558,152
_4114
Pf1N1B4_4115 - FIG074102: hypothetical protein, at 4,558,250 to 4,558,729
_4115
Position (kb) 4557
4558 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4556.378 kb on + strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.188 kb on - strand at 4557.194 kb on + strand at 4557.231 kb on + strand at 4557.231 kb on + strand at 4557.231 kb on + strand at 4557.232 kb on - strand at 4557.291 kb on - strand at 4557.328 kb on + strand at 4557.329 kb on - strand at 4557.423 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.472 kb on - strand, within Pf1N1B4_4112 at 4557.472 kb on - strand, within Pf1N1B4_4112 at 4557.472 kb on - strand, within Pf1N1B4_4112 at 4557.549 kb on - strand at 4557.971 kb on + strand at 4557.972 kb on - strand, within Pf1N1B4_4113 at 4557.972 kb on - strand, within Pf1N1B4_4113 at 4558.062 kb on - strand, within Pf1N1B4_4114 at 4558.062 kb on - strand, within Pf1N1B4_4114 at 4558.554 kb on + strand, within Pf1N1B4_4115
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 4,556,378 + -0.5 4,556,379 - +1.3 4,556,379 - -1.9 4,556,379 - +2.5 4,556,379 - -0.3 4,556,379 - +0.3 4,556,474 + Pf1N1B4_4110 0.30 -1.9 4,556,474 + Pf1N1B4_4110 0.30 +0.7 4,556,475 - Pf1N1B4_4110 0.31 -0.2 4,556,475 - Pf1N1B4_4110 0.31 -3.3 4,557,020 - Pf1N1B4_4111 0.48 -0.5 4,557,020 - Pf1N1B4_4111 0.48 -0.2 4,557,020 - Pf1N1B4_4111 0.48 -0.3 4,557,020 - Pf1N1B4_4111 0.48 -0.3 4,557,020 - Pf1N1B4_4111 0.48 -1.1 4,557,188 - +0.3 4,557,194 + +0.7 4,557,231 + +0.7 4,557,231 + -2.3 4,557,231 + -0.6 4,557,232 - +0.3 4,557,291 - +1.3 4,557,328 + +0.3 4,557,329 - -0.3 4,557,423 + Pf1N1B4_4112 0.44 +0.3 4,557,425 + Pf1N1B4_4112 0.44 -2.3 4,557,425 + +0.7 4,557,425 + Pf1N1B4_4112 0.44 -1.0 4,557,425 + Pf1N1B4_4112 0.44 +0.9 4,557,425 + Pf1N1B4_4112 0.44 -2.3 4,557,425 + Pf1N1B4_4112 0.44 +1.0 4,557,425 + Pf1N1B4_4112 0.44 +0.3 4,557,425 + Pf1N1B4_4112 0.44 +0.2 4,557,425 + Pf1N1B4_4112 0.44 +0.1 4,557,426 - Pf1N1B4_4112 0.45 +0.3 4,557,426 - Pf1N1B4_4112 0.45 -1.2 4,557,426 - Pf1N1B4_4112 0.45 -1.3 4,557,426 - Pf1N1B4_4112 0.45 -1.7 4,557,426 - Pf1N1B4_4112 0.45 -0.8 4,557,472 - Pf1N1B4_4112 0.63 +0.3 4,557,472 - Pf1N1B4_4112 0.63 -0.7 4,557,472 - Pf1N1B4_4112 0.63 -1.7 4,557,549 - -1.9 4,557,971 + +1.0 4,557,972 - Pf1N1B4_4113 0.86 -0.3 4,557,972 - Pf1N1B4_4113 0.86 +0.7 4,558,062 - Pf1N1B4_4114 0.20 -0.1 4,558,062 - Pf1N1B4_4114 0.20 +2.0 4,558,554 + Pf1N1B4_4115 0.63 -0.8
Or see this region's nucleotide sequence