Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_34 and Pf1N1B4_35 are separated by 432 nucleotides Pf1N1B4_35 and Pf1N1B4_36 are separated by 3 nucleotides Pf1N1B4_36 and Pf1N1B4_37 are separated by 81 nucleotides
Pf1N1B4_34 - Xanthine permease, at 39,575 to 41,029
_34
Pf1N1B4_35 - hypothetical protein, at 41,462 to 41,629
_35
Pf1N1B4_36 - hypothetical protein, at 41,633 to 41,977
_36
Pf1N1B4_37 - N-hydroxyarylamine O-acetyltransferase (EC 2.3.1.118), at 42,059 to 42,898
_37
Position (kb) 41
42 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.995 kb on + strand at 40.995 kb on + strand at 40.996 kb on - strand at 40.996 kb on - strand at 40.996 kb on - strand at 41.030 kb on - strand at 41.133 kb on - strand at 41.167 kb on - strand at 41.169 kb on - strand at 41.249 kb on + strand at 41.250 kb on - strand at 41.250 kb on - strand at 41.250 kb on - strand at 41.330 kb on - strand at 41.336 kb on - strand at 41.336 kb on - strand at 41.543 kb on + strand, within Pf1N1B4_35 at 41.544 kb on - strand at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.549 kb on + strand at 41.615 kb on + strand at 41.615 kb on + strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.618 kb on - strand at 41.618 kb on - strand at 41.646 kb on - strand at 41.731 kb on - strand, within Pf1N1B4_36 at 41.791 kb on + strand, within Pf1N1B4_36 at 41.792 kb on - strand, within Pf1N1B4_36 at 41.975 kb on + strand at 41.976 kb on - strand at 42.233 kb on - strand, within Pf1N1B4_37 at 42.275 kb on + strand, within Pf1N1B4_37 at 42.315 kb on + strand, within Pf1N1B4_37 at 42.316 kb on - strand, within Pf1N1B4_37 at 42.316 kb on - strand, within Pf1N1B4_37 at 42.369 kb on + strand, within Pf1N1B4_37 at 42.369 kb on + strand, within Pf1N1B4_37 at 42.369 kb on + strand, within Pf1N1B4_37 at 42.369 kb on + strand, within Pf1N1B4_37 at 42.370 kb on - strand, within Pf1N1B4_37 at 42.370 kb on - strand, within Pf1N1B4_37 at 42.370 kb on - strand, within Pf1N1B4_37 at 42.370 kb on - strand, within Pf1N1B4_37 at 42.411 kb on + strand, within Pf1N1B4_37 at 42.528 kb on + strand, within Pf1N1B4_37 at 42.529 kb on - strand, within Pf1N1B4_37
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 40,501 + Pf1N1B4_34 0.64 +0.9 40,501 + Pf1N1B4_34 0.64 +1.8 40,501 + Pf1N1B4_34 0.64 -0.0 40,995 + -0.2 40,995 + -1.0 40,996 - -0.5 40,996 - -1.0 40,996 - +0.4 41,030 - -1.2 41,133 - -0.6 41,167 - +2.1 41,169 - -0.6 41,249 + -0.2 41,250 - -0.1 41,250 - +0.4 41,250 - +1.8 41,330 - -0.2 41,336 - -0.6 41,336 - +0.1 41,543 + Pf1N1B4_35 0.48 -0.8 41,544 - +1.8 41,544 - Pf1N1B4_35 0.49 -2.6 41,544 - Pf1N1B4_35 0.49 -0.2 41,544 - Pf1N1B4_35 0.49 -2.6 41,544 - Pf1N1B4_35 0.49 -2.2 41,544 - Pf1N1B4_35 0.49 -0.8 41,544 - Pf1N1B4_35 0.49 -0.2 41,544 - Pf1N1B4_35 0.49 +0.1 41,549 + +0.2 41,615 + -1.2 41,615 + -0.2 41,616 - +0.9 41,616 - -0.2 41,616 - +1.2 41,616 - +2.8 41,616 - -0.0 41,616 - +0.4 41,616 - -2.0 41,618 - +0.8 41,618 - -0.2 41,646 - +0.4 41,731 - Pf1N1B4_36 0.28 +1.4 41,791 + Pf1N1B4_36 0.46 +1.1 41,792 - Pf1N1B4_36 0.46 +1.4 41,975 + -1.8 41,976 - -1.2 42,233 - Pf1N1B4_37 0.21 -0.0 42,275 + Pf1N1B4_37 0.26 +0.4 42,315 + Pf1N1B4_37 0.30 -0.5 42,316 - Pf1N1B4_37 0.31 -1.2 42,316 - Pf1N1B4_37 0.31 -1.8 42,369 + Pf1N1B4_37 0.37 +1.6 42,369 + Pf1N1B4_37 0.37 -1.8 42,369 + Pf1N1B4_37 0.37 +0.6 42,369 + Pf1N1B4_37 0.37 -2.2 42,370 - Pf1N1B4_37 0.37 -0.4 42,370 - Pf1N1B4_37 0.37 +0.8 42,370 - Pf1N1B4_37 0.37 -1.2 42,370 - Pf1N1B4_37 0.37 -0.8 42,411 + Pf1N1B4_37 0.42 +0.5 42,528 + Pf1N1B4_37 0.56 -0.2 42,529 - Pf1N1B4_37 0.56 -0.2
Or see this region's nucleotide sequence