Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3465 and Pf1N1B4_3466 are separated by 63 nucleotides Pf1N1B4_3466 and Pf1N1B4_3467 are separated by 88 nucleotides Pf1N1B4_3467 and Pf1N1B4_3468 are separated by 47 nucleotides
Pf1N1B4_3465 - Transporter, MFS superfamily, at 3,871,796 to 3,873,178
_3465
Pf1N1B4_3466 - FIG00955605: hypothetical protein, at 3,873,242 to 3,873,988
_3466
Pf1N1B4_3467 - Flagellar biosynthesis protein FlgN, at 3,874,077 to 3,874,544
_3467
Pf1N1B4_3468 - Negative regulator of flagellin synthesis FlgM, at 3,874,592 to 3,874,918
_3468
Position (kb)
3873
3874 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3872.410 kb on + strand, within Pf1N1B4_3465 at 3872.447 kb on + strand, within Pf1N1B4_3465 at 3872.448 kb on - strand, within Pf1N1B4_3465 at 3872.465 kb on + strand, within Pf1N1B4_3465 at 3872.465 kb on + strand, within Pf1N1B4_3465 at 3872.465 kb on + strand, within Pf1N1B4_3465 at 3872.466 kb on - strand, within Pf1N1B4_3465 at 3872.466 kb on - strand, within Pf1N1B4_3465 at 3872.515 kb on + strand, within Pf1N1B4_3465 at 3872.516 kb on - strand at 3872.574 kb on - strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.792 kb on + strand, within Pf1N1B4_3465 at 3872.793 kb on - strand, within Pf1N1B4_3465 at 3872.793 kb on - strand, within Pf1N1B4_3465 at 3872.793 kb on - strand, within Pf1N1B4_3465 at 3872.793 kb on - strand at 3872.793 kb on - strand, within Pf1N1B4_3465 at 3872.793 kb on - strand, within Pf1N1B4_3465 at 3872.793 kb on - strand at 3872.793 kb on - strand, within Pf1N1B4_3465 at 3872.793 kb on - strand, within Pf1N1B4_3465 at 3872.864 kb on + strand, within Pf1N1B4_3465 at 3872.865 kb on - strand, within Pf1N1B4_3465 at 3872.865 kb on - strand at 3872.865 kb on - strand, within Pf1N1B4_3465 at 3872.865 kb on - strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.980 kb on + strand, within Pf1N1B4_3465 at 3872.981 kb on - strand, within Pf1N1B4_3465 at 3872.981 kb on - strand, within Pf1N1B4_3465 at 3873.240 kb on + strand at 3873.288 kb on + strand at 3873.288 kb on + strand at 3873.289 kb on - strand at 3873.289 kb on - strand at 3873.289 kb on - strand at 3873.385 kb on - strand, within Pf1N1B4_3466 at 3873.624 kb on + strand at 3873.625 kb on - strand, within Pf1N1B4_3466 at 3873.654 kb on + strand, within Pf1N1B4_3466 at 3873.716 kb on + strand, within Pf1N1B4_3466 at 3873.837 kb on + strand, within Pf1N1B4_3466 at 3873.838 kb on - strand, within Pf1N1B4_3466 at 3873.934 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.012 kb on - strand at 3874.012 kb on - strand at 3874.012 kb on - strand at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.136 kb on - strand, within Pf1N1B4_3467 at 3874.136 kb on - strand, within Pf1N1B4_3467 at 3874.220 kb on + strand, within Pf1N1B4_3467 at 3874.527 kb on + strand at 3874.527 kb on + strand at 3874.532 kb on + strand at 3874.532 kb on + strand at 3874.533 kb on - strand at 3874.649 kb on - strand, within Pf1N1B4_3468 at 3874.650 kb on + strand, within Pf1N1B4_3468 at 3874.650 kb on + strand, within Pf1N1B4_3468 at 3874.861 kb on + strand, within Pf1N1B4_3468 at 3874.936 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 3,872,410 + Pf1N1B4_3465 0.44 -0.7 3,872,447 + Pf1N1B4_3465 0.47 +0.4 3,872,448 - Pf1N1B4_3465 0.47 +0.2 3,872,465 + Pf1N1B4_3465 0.48 +0.4 3,872,465 + Pf1N1B4_3465 0.48 -0.1 3,872,465 + Pf1N1B4_3465 0.48 -0.1 3,872,466 - Pf1N1B4_3465 0.48 +0.1 3,872,466 - Pf1N1B4_3465 0.48 -1.7 3,872,515 + Pf1N1B4_3465 0.52 +0.5 3,872,516 - -1.1 3,872,574 - Pf1N1B4_3465 0.56 +0.4 3,872,792 + Pf1N1B4_3465 0.72 +1.9 3,872,792 + Pf1N1B4_3465 0.72 +1.0 3,872,792 + Pf1N1B4_3465 0.72 -0.7 3,872,792 + Pf1N1B4_3465 0.72 -0.7 3,872,792 + Pf1N1B4_3465 0.72 -0.6 3,872,792 + Pf1N1B4_3465 0.72 -0.9 3,872,792 + Pf1N1B4_3465 0.72 +1.1 3,872,792 + Pf1N1B4_3465 0.72 +1.2 3,872,792 + Pf1N1B4_3465 0.72 +0.3 3,872,793 - Pf1N1B4_3465 0.72 -0.7 3,872,793 - Pf1N1B4_3465 0.72 +0.3 3,872,793 - Pf1N1B4_3465 0.72 -1.7 3,872,793 - -0.1 3,872,793 - Pf1N1B4_3465 0.72 +0.2 3,872,793 - Pf1N1B4_3465 0.72 +1.4 3,872,793 - -1.1 3,872,793 - Pf1N1B4_3465 0.72 +1.3 3,872,793 - Pf1N1B4_3465 0.72 +1.9 3,872,864 + Pf1N1B4_3465 0.77 -1.7 3,872,865 - Pf1N1B4_3465 0.77 +1.9 3,872,865 - +0.4 3,872,865 - Pf1N1B4_3465 0.77 -0.1 3,872,865 - Pf1N1B4_3465 0.77 -1.1 3,872,980 + Pf1N1B4_3465 0.86 +0.3 3,872,980 + Pf1N1B4_3465 0.86 -2.1 3,872,980 + Pf1N1B4_3465 0.86 -0.9 3,872,980 + Pf1N1B4_3465 0.86 -1.7 3,872,980 + Pf1N1B4_3465 0.86 -0.0 3,872,980 + Pf1N1B4_3465 0.86 -0.0 3,872,980 + Pf1N1B4_3465 0.86 -0.1 3,872,980 + Pf1N1B4_3465 0.86 +0.6 3,872,980 + Pf1N1B4_3465 0.86 -0.8 3,872,981 - Pf1N1B4_3465 0.86 -1.1 3,872,981 - Pf1N1B4_3465 0.86 -2.7 3,873,240 + -0.1 3,873,288 + -1.5 3,873,288 + +0.3 3,873,289 - -1.1 3,873,289 - -0.3 3,873,289 - -0.4 3,873,385 - Pf1N1B4_3466 0.19 -1.5 3,873,624 + -1.1 3,873,625 - Pf1N1B4_3466 0.51 -2.5 3,873,654 + Pf1N1B4_3466 0.55 -1.7 3,873,716 + Pf1N1B4_3466 0.63 +0.6 3,873,837 + Pf1N1B4_3466 0.80 -0.9 3,873,838 - Pf1N1B4_3466 0.80 +3.0 3,873,934 + -0.2 3,874,011 + -0.1 3,874,011 + -1.0 3,874,011 + +1.2 3,874,011 + -1.1 3,874,011 + -0.1 3,874,011 + +0.4 3,874,011 + -0.4 3,874,011 + +1.4 3,874,011 + +0.2 3,874,011 + -0.3 3,874,011 + -0.3 3,874,011 + +0.3 3,874,011 + +0.4 3,874,011 + +1.2 3,874,011 + +0.4 3,874,011 + -2.6 3,874,011 + +2.2 3,874,011 + -1.7 3,874,011 + -0.1 3,874,011 + +0.9 3,874,011 + +1.2 3,874,012 - -0.1 3,874,012 - +1.4 3,874,012 - +1.2 3,874,135 + Pf1N1B4_3467 0.12 +0.2 3,874,135 + Pf1N1B4_3467 0.12 -0.1 3,874,135 + Pf1N1B4_3467 0.12 -1.5 3,874,135 + Pf1N1B4_3467 0.12 +1.4 3,874,136 - Pf1N1B4_3467 0.13 +2.2 3,874,136 - Pf1N1B4_3467 0.13 -0.1 3,874,220 + Pf1N1B4_3467 0.31 -0.6 3,874,527 + +0.9 3,874,527 + +0.9 3,874,532 + -1.3 3,874,532 + -0.5 3,874,533 - +0.9 3,874,649 - Pf1N1B4_3468 0.17 +0.3 3,874,650 + Pf1N1B4_3468 0.18 +0.3 3,874,650 + Pf1N1B4_3468 0.18 +0.6 3,874,861 + Pf1N1B4_3468 0.82 -1.1 3,874,936 + -1.7
Or see this region's nucleotide sequence