Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3329 and Pf1N1B4_3330 are separated by 112 nucleotides Pf1N1B4_3330 and Pf1N1B4_3331 are separated by 10 nucleotides Pf1N1B4_3331 and Pf1N1B4_3332 are separated by 326 nucleotides
Pf1N1B4_3329 - Putative permease PerM (= YfgO), at 3,717,239 to 3,718,309
_3329
Pf1N1B4_3330 - Thiol peroxidase, Bcp-type (EC 1.11.1.15), at 3,718,422 to 3,718,895
_3330
Pf1N1B4_3331 - Glycine cleavage system transcriptional antiactivator GcvR, at 3,718,906 to 3,719,466
_3331
Pf1N1B4_3332 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 3,719,793 to 3,720,671
_3332
Position (kb)
3718
3719
3720 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand at 3717.920 kb on + strand at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.950 kb on + strand, within Pf1N1B4_3329 at 3717.950 kb on + strand, within Pf1N1B4_3329 at 3717.951 kb on - strand, within Pf1N1B4_3329 at 3718.076 kb on + strand, within Pf1N1B4_3329 at 3718.076 kb on + strand, within Pf1N1B4_3329 at 3718.221 kb on + strand at 3718.307 kb on + strand at 3718.307 kb on + strand at 3718.352 kb on - strand at 3718.375 kb on - strand at 3718.375 kb on - strand at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.706 kb on + strand, within Pf1N1B4_3330 at 3718.719 kb on - strand, within Pf1N1B4_3330 at 3718.787 kb on - strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.793 kb on - strand at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.907 kb on + strand at 3718.907 kb on + strand at 3718.908 kb on - strand at 3718.908 kb on - strand at 3718.908 kb on - strand at 3719.069 kb on + strand, within Pf1N1B4_3331 at 3719.069 kb on + strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.165 kb on + strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.174 kb on + strand, within Pf1N1B4_3331 at 3719.175 kb on - strand at 3719.496 kb on + strand at 3719.658 kb on + strand at 3719.658 kb on + strand at 3719.659 kb on - strand at 3719.725 kb on - strand at 3719.755 kb on + strand at 3720.111 kb on + strand at 3720.138 kb on + strand, within Pf1N1B4_3332 at 3720.189 kb on + strand at 3720.398 kb on + strand, within Pf1N1B4_3332 at 3720.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 3,717,920 + Pf1N1B4_3329 0.64 -0.1 3,717,920 + Pf1N1B4_3329 0.64 -2.1 3,717,920 + -1.1 3,717,920 + +1.1 3,717,920 + Pf1N1B4_3329 0.64 +1.2 3,717,920 + Pf1N1B4_3329 0.64 -0.7 3,717,920 + Pf1N1B4_3329 0.64 +0.7 3,717,920 + Pf1N1B4_3329 0.64 +0.3 3,717,950 + Pf1N1B4_3329 0.66 -1.1 3,717,950 + Pf1N1B4_3329 0.66 -0.5 3,717,951 - Pf1N1B4_3329 0.66 -0.1 3,718,076 + Pf1N1B4_3329 0.78 -1.0 3,718,076 + Pf1N1B4_3329 0.78 -2.4 3,718,221 + -0.7 3,718,307 + +0.9 3,718,307 + +0.5 3,718,352 - +1.5 3,718,375 - -1.1 3,718,375 - -1.7 3,718,552 + Pf1N1B4_3330 0.27 +0.5 3,718,552 + Pf1N1B4_3330 0.27 -0.1 3,718,552 + Pf1N1B4_3330 0.27 -1.7 3,718,552 + Pf1N1B4_3330 0.27 -0.1 3,718,553 - Pf1N1B4_3330 0.28 +0.8 3,718,553 - Pf1N1B4_3330 0.28 +0.9 3,718,553 - Pf1N1B4_3330 0.28 +0.1 3,718,706 + Pf1N1B4_3330 0.60 -0.1 3,718,719 - Pf1N1B4_3330 0.63 +0.6 3,718,787 - Pf1N1B4_3330 0.77 +1.9 3,718,792 + Pf1N1B4_3330 0.78 +0.4 3,718,792 + Pf1N1B4_3330 0.78 +0.3 3,718,792 + Pf1N1B4_3330 0.78 -0.4 3,718,792 + Pf1N1B4_3330 0.78 -0.1 3,718,793 - +0.9 3,718,793 - Pf1N1B4_3330 0.78 +0.5 3,718,793 - Pf1N1B4_3330 0.78 -0.3 3,718,793 - -0.1 3,718,793 - Pf1N1B4_3330 0.78 -0.1 3,718,793 - Pf1N1B4_3330 0.78 +0.5 3,718,793 - Pf1N1B4_3330 0.78 -2.1 3,718,907 + +0.5 3,718,907 + -0.1 3,718,908 - -0.7 3,718,908 - +0.3 3,718,908 - -1.7 3,719,069 + Pf1N1B4_3331 0.29 -1.3 3,719,069 + Pf1N1B4_3331 0.29 +0.9 3,719,070 - Pf1N1B4_3331 0.29 -0.7 3,719,070 - Pf1N1B4_3331 0.29 -2.1 3,719,070 - Pf1N1B4_3331 0.29 -0.1 3,719,165 + Pf1N1B4_3331 0.46 -0.9 3,719,166 - Pf1N1B4_3331 0.46 -1.7 3,719,166 - Pf1N1B4_3331 0.46 -1.4 3,719,166 - Pf1N1B4_3331 0.46 -2.4 3,719,174 + Pf1N1B4_3331 0.48 +1.5 3,719,175 - +1.9 3,719,496 + -1.1 3,719,658 + +0.9 3,719,658 + -1.7 3,719,659 - -2.7 3,719,725 - -0.1 3,719,755 + -0.8 3,720,111 + +0.9 3,720,138 + Pf1N1B4_3332 0.39 -1.1 3,720,189 + -0.1 3,720,398 + Pf1N1B4_3332 0.69 +1.2 3,720,398 + -1.1
Or see this region's nucleotide sequence