Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3241 and Pf1N1B4_3242 are separated by 192 nucleotides Pf1N1B4_3242 and Pf1N1B4_3243 are separated by 15 nucleotides
Pf1N1B4_3241 - hypothetical protein, at 3,628,149 to 3,628,412
_3241
Pf1N1B4_3242 - GCN5-related N-acetyltransferase, at 3,628,605 to 3,629,000
_3242
Pf1N1B4_3243 - hypothetical protein, at 3,629,016 to 3,630,176
_3243
Position (kb)
3628
3629
3630 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3627.685 kb on + strand at 3627.685 kb on + strand at 3627.686 kb on - strand at 3627.767 kb on + strand at 3627.807 kb on + strand at 3627.812 kb on - strand at 3628.158 kb on - strand at 3628.228 kb on + strand, within Pf1N1B4_3241 at 3628.273 kb on + strand, within Pf1N1B4_3241 at 3628.273 kb on + strand, within Pf1N1B4_3241 at 3628.274 kb on - strand, within Pf1N1B4_3241 at 3628.277 kb on + strand, within Pf1N1B4_3241 at 3628.478 kb on + strand at 3628.478 kb on + strand at 3628.478 kb on + strand at 3628.481 kb on + strand at 3628.481 kb on + strand at 3628.481 kb on + strand at 3628.482 kb on - strand at 3628.482 kb on - strand at 3628.565 kb on + strand at 3628.566 kb on - strand at 3628.747 kb on + strand, within Pf1N1B4_3242 at 3628.800 kb on - strand, within Pf1N1B4_3242 at 3628.800 kb on - strand at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.804 kb on - strand, within Pf1N1B4_3242 at 3628.804 kb on - strand, within Pf1N1B4_3242 at 3628.948 kb on + strand, within Pf1N1B4_3242 at 3628.949 kb on - strand, within Pf1N1B4_3242 at 3629.174 kb on - strand, within Pf1N1B4_3243 at 3629.174 kb on - strand, within Pf1N1B4_3243 at 3629.182 kb on + strand, within Pf1N1B4_3243 at 3629.235 kb on - strand, within Pf1N1B4_3243 at 3629.237 kb on - strand, within Pf1N1B4_3243 at 3629.271 kb on - strand, within Pf1N1B4_3243 at 3629.433 kb on + strand, within Pf1N1B4_3243 at 3629.457 kb on - strand, within Pf1N1B4_3243 at 3629.787 kb on + strand, within Pf1N1B4_3243 at 3629.787 kb on + strand, within Pf1N1B4_3243 at 3629.787 kb on + strand, within Pf1N1B4_3243 at 3629.788 kb on - strand, within Pf1N1B4_3243 at 3629.788 kb on - strand, within Pf1N1B4_3243 at 3629.789 kb on + strand, within Pf1N1B4_3243 at 3629.789 kb on + strand, within Pf1N1B4_3243 at 3629.789 kb on + strand, within Pf1N1B4_3243 at 3629.790 kb on - strand, within Pf1N1B4_3243 at 3629.798 kb on - strand, within Pf1N1B4_3243 at 3629.798 kb on - strand, within Pf1N1B4_3243 at 3629.798 kb on - strand at 3629.846 kb on + strand, within Pf1N1B4_3243 at 3629.846 kb on + strand, within Pf1N1B4_3243 at 3629.846 kb on + strand, within Pf1N1B4_3243 at 3629.847 kb on - strand at 3629.847 kb on - strand, within Pf1N1B4_3243 at 3629.880 kb on + strand, within Pf1N1B4_3243 at 3629.881 kb on - strand, within Pf1N1B4_3243 at 3629.881 kb on - strand, within Pf1N1B4_3243 at 3629.925 kb on + strand, within Pf1N1B4_3243 at 3629.925 kb on + strand, within Pf1N1B4_3243 at 3629.925 kb on + strand, within Pf1N1B4_3243 at 3629.926 kb on - strand, within Pf1N1B4_3243 at 3629.926 kb on - strand, within Pf1N1B4_3243 at 3629.926 kb on - strand, within Pf1N1B4_3243 at 3629.926 kb on - strand, within Pf1N1B4_3243 at 3629.926 kb on - strand, within Pf1N1B4_3243 at 3629.926 kb on - strand, within Pf1N1B4_3243
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 3,627,685 + +3.1 3,627,685 + -1.1 3,627,686 - +0.9 3,627,767 + +0.3 3,627,807 + -2.4 3,627,812 - -0.6 3,628,158 - -2.1 3,628,228 + Pf1N1B4_3241 0.30 -0.3 3,628,273 + Pf1N1B4_3241 0.47 -1.6 3,628,273 + Pf1N1B4_3241 0.47 -0.1 3,628,274 - Pf1N1B4_3241 0.47 -1.1 3,628,277 + Pf1N1B4_3241 0.48 +1.5 3,628,478 + +1.5 3,628,478 + +0.5 3,628,478 + +0.9 3,628,481 + -0.3 3,628,481 + -0.6 3,628,481 + +0.3 3,628,482 - -1.2 3,628,482 - -2.6 3,628,565 + -0.8 3,628,566 - -1.6 3,628,747 + Pf1N1B4_3242 0.36 -2.6 3,628,800 - Pf1N1B4_3242 0.49 -1.1 3,628,800 - +0.5 3,628,803 + Pf1N1B4_3242 0.50 -1.1 3,628,803 + Pf1N1B4_3242 0.50 +0.2 3,628,803 + Pf1N1B4_3242 0.50 -1.1 3,628,803 + Pf1N1B4_3242 0.50 -0.1 3,628,803 + Pf1N1B4_3242 0.50 +2.5 3,628,803 + Pf1N1B4_3242 0.50 -3.1 3,628,804 - Pf1N1B4_3242 0.50 +0.7 3,628,804 - Pf1N1B4_3242 0.50 -0.1 3,628,948 + Pf1N1B4_3242 0.87 -1.4 3,628,949 - Pf1N1B4_3242 0.87 -1.6 3,629,174 - Pf1N1B4_3243 0.14 +0.8 3,629,174 - Pf1N1B4_3243 0.14 -0.1 3,629,182 + Pf1N1B4_3243 0.14 -1.0 3,629,235 - Pf1N1B4_3243 0.19 +0.5 3,629,237 - Pf1N1B4_3243 0.19 +0.4 3,629,271 - Pf1N1B4_3243 0.22 +0.5 3,629,433 + Pf1N1B4_3243 0.36 -1.1 3,629,457 - Pf1N1B4_3243 0.38 -0.1 3,629,787 + Pf1N1B4_3243 0.66 -0.1 3,629,787 + Pf1N1B4_3243 0.66 -0.1 3,629,787 + Pf1N1B4_3243 0.66 +0.1 3,629,788 - Pf1N1B4_3243 0.66 +0.2 3,629,788 - Pf1N1B4_3243 0.66 -0.8 3,629,789 + Pf1N1B4_3243 0.67 -1.4 3,629,789 + Pf1N1B4_3243 0.67 +0.1 3,629,789 + Pf1N1B4_3243 0.67 +0.4 3,629,790 - Pf1N1B4_3243 0.67 -0.5 3,629,798 - Pf1N1B4_3243 0.67 -0.1 3,629,798 - Pf1N1B4_3243 0.67 -0.5 3,629,798 - -1.1 3,629,846 + Pf1N1B4_3243 0.71 -0.4 3,629,846 + Pf1N1B4_3243 0.71 -1.4 3,629,846 + Pf1N1B4_3243 0.71 -1.6 3,629,847 - -0.1 3,629,847 - Pf1N1B4_3243 0.72 -1.1 3,629,880 + Pf1N1B4_3243 0.74 -1.3 3,629,881 - Pf1N1B4_3243 0.75 -2.1 3,629,881 - Pf1N1B4_3243 0.75 +1.9 3,629,925 + Pf1N1B4_3243 0.78 +0.9 3,629,925 + Pf1N1B4_3243 0.78 -1.6 3,629,925 + Pf1N1B4_3243 0.78 +0.3 3,629,926 - Pf1N1B4_3243 0.78 -0.5 3,629,926 - Pf1N1B4_3243 0.78 -1.4 3,629,926 - Pf1N1B4_3243 0.78 -0.1 3,629,926 - Pf1N1B4_3243 0.78 -0.4 3,629,926 - Pf1N1B4_3243 0.78 +0.7 3,629,926 - Pf1N1B4_3243 0.78 -0.6
Or see this region's nucleotide sequence