Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3123 and Pf1N1B4_3124 are separated by 67 nucleotides Pf1N1B4_3124 and Pf1N1B4_3125 overlap by 4 nucleotides Pf1N1B4_3125 and Pf1N1B4_3126 are separated by 127 nucleotides
Pf1N1B4_3123 - Bacteriophage tail sheath protein, at 3,508,588 to 3,510,084
_3123
Pf1N1B4_3124 - Phage tail tube protein, at 3,510,152 to 3,510,499
_3124
Pf1N1B4_3125 - FIG00954242: hypothetical protein, at 3,510,496 to 3,510,792
_3125
Pf1N1B4_3126 - Phage tail length tape-measure protein, at 3,510,920 to 3,512,692
_3126
Position (kb)
3510
3511 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3509.633 kb on - strand, within Pf1N1B4_3123 at 3509.633 kb on - strand, within Pf1N1B4_3123 at 3509.650 kb on + strand, within Pf1N1B4_3123 at 3509.650 kb on + strand, within Pf1N1B4_3123 at 3509.651 kb on - strand, within Pf1N1B4_3123 at 3509.651 kb on - strand, within Pf1N1B4_3123 at 3509.653 kb on + strand, within Pf1N1B4_3123 at 3509.653 kb on + strand, within Pf1N1B4_3123 at 3509.666 kb on - strand, within Pf1N1B4_3123 at 3509.666 kb on - strand, within Pf1N1B4_3123 at 3509.695 kb on + strand, within Pf1N1B4_3123 at 3509.695 kb on + strand, within Pf1N1B4_3123 at 3509.695 kb on + strand, within Pf1N1B4_3123 at 3509.696 kb on - strand, within Pf1N1B4_3123 at 3509.696 kb on - strand, within Pf1N1B4_3123 at 3509.731 kb on + strand, within Pf1N1B4_3123 at 3509.732 kb on - strand, within Pf1N1B4_3123 at 3509.732 kb on - strand, within Pf1N1B4_3123 at 3509.799 kb on + strand, within Pf1N1B4_3123 at 3509.803 kb on + strand, within Pf1N1B4_3123 at 3509.803 kb on + strand, within Pf1N1B4_3123 at 3509.803 kb on + strand, within Pf1N1B4_3123 at 3509.804 kb on - strand, within Pf1N1B4_3123 at 3509.804 kb on - strand, within Pf1N1B4_3123 at 3510.016 kb on + strand at 3510.016 kb on + strand at 3510.016 kb on + strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.068 kb on - strand at 3510.132 kb on + strand at 3510.132 kb on + strand at 3510.133 kb on - strand at 3510.133 kb on - strand at 3510.179 kb on + strand at 3510.179 kb on + strand at 3510.179 kb on + strand at 3510.180 kb on - strand at 3510.209 kb on - strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.398 kb on + strand, within Pf1N1B4_3124 at 3510.416 kb on + strand, within Pf1N1B4_3124 at 3510.611 kb on - strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.805 kb on - strand at 3510.805 kb on - strand at 3510.805 kb on - strand at 3511.063 kb on + strand at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 3,509,633 - Pf1N1B4_3123 0.70 -1.0 3,509,633 - Pf1N1B4_3123 0.70 +2.0 3,509,650 + Pf1N1B4_3123 0.71 -1.2 3,509,650 + Pf1N1B4_3123 0.71 -0.0 3,509,651 - Pf1N1B4_3123 0.71 -1.0 3,509,651 - Pf1N1B4_3123 0.71 +0.7 3,509,653 + Pf1N1B4_3123 0.71 -0.2 3,509,653 + Pf1N1B4_3123 0.71 +1.0 3,509,666 - Pf1N1B4_3123 0.72 -1.0 3,509,666 - Pf1N1B4_3123 0.72 +0.3 3,509,695 + Pf1N1B4_3123 0.74 +1.0 3,509,695 + Pf1N1B4_3123 0.74 -1.0 3,509,695 + Pf1N1B4_3123 0.74 -0.0 3,509,696 - Pf1N1B4_3123 0.74 +3.2 3,509,696 - Pf1N1B4_3123 0.74 +0.4 3,509,731 + Pf1N1B4_3123 0.76 -1.6 3,509,732 - Pf1N1B4_3123 0.76 +0.2 3,509,732 - Pf1N1B4_3123 0.76 -1.6 3,509,799 + Pf1N1B4_3123 0.81 -0.6 3,509,803 + Pf1N1B4_3123 0.81 -2.0 3,509,803 + Pf1N1B4_3123 0.81 +1.2 3,509,803 + Pf1N1B4_3123 0.81 +1.0 3,509,804 - Pf1N1B4_3123 0.81 -0.4 3,509,804 - Pf1N1B4_3123 0.81 -0.0 3,510,016 + +0.4 3,510,016 + +2.8 3,510,016 + -1.0 3,510,017 - -0.6 3,510,017 - +1.0 3,510,017 - -1.0 3,510,017 - +0.6 3,510,017 - -0.0 3,510,067 + -0.6 3,510,067 + -0.4 3,510,067 + -1.6 3,510,067 + -1.0 3,510,068 - +1.6 3,510,132 + -0.0 3,510,132 + -0.0 3,510,133 - -0.4 3,510,133 - +0.6 3,510,179 + +1.3 3,510,179 + -0.0 3,510,179 + -1.6 3,510,180 - -0.4 3,510,209 - Pf1N1B4_3124 0.16 +1.6 3,510,272 + Pf1N1B4_3124 0.34 +1.6 3,510,272 + Pf1N1B4_3124 0.34 +0.4 3,510,272 + Pf1N1B4_3124 0.34 -1.0 3,510,273 - Pf1N1B4_3124 0.35 -0.0 3,510,273 - Pf1N1B4_3124 0.35 +0.7 3,510,273 - Pf1N1B4_3124 0.35 -0.0 3,510,273 - Pf1N1B4_3124 0.35 +0.5 3,510,273 - Pf1N1B4_3124 0.35 -1.0 3,510,273 - Pf1N1B4_3124 0.35 +0.4 3,510,273 - Pf1N1B4_3124 0.35 +0.4 3,510,398 + Pf1N1B4_3124 0.71 +1.3 3,510,416 + Pf1N1B4_3124 0.76 +0.8 3,510,611 - Pf1N1B4_3125 0.39 -0.9 3,510,664 + Pf1N1B4_3125 0.57 -1.6 3,510,664 + Pf1N1B4_3125 0.57 -0.7 3,510,664 + Pf1N1B4_3125 0.57 -1.3 3,510,664 + Pf1N1B4_3125 0.57 -0.6 3,510,664 + Pf1N1B4_3125 0.57 -1.4 3,510,664 + Pf1N1B4_3125 0.57 +0.7 3,510,665 - Pf1N1B4_3125 0.57 -0.4 3,510,665 - Pf1N1B4_3125 0.57 +0.4 3,510,665 - Pf1N1B4_3125 0.57 +2.3 3,510,805 - -0.6 3,510,805 - +2.0 3,510,805 - -1.0 3,511,063 + -0.6 3,511,422 + Pf1N1B4_3126 0.28 -1.0 3,511,422 + Pf1N1B4_3126 0.28 -0.6 3,511,422 + Pf1N1B4_3126 0.28 +0.4 3,511,756 + Pf1N1B4_3126 0.47 +1.0 3,511,756 + Pf1N1B4_3126 0.47 -0.3 3,511,756 + Pf1N1B4_3126 0.47 +2.3
Or see this region's nucleotide sequence