Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2440 and Pf1N1B4_2441 overlap by 1 nucleotides Pf1N1B4_2441 and Pf1N1B4_2442 are separated by 15 nucleotides Pf1N1B4_2442 and Pf1N1B4_2443 are separated by 188 nucleotides
Pf1N1B4_2440 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 2,764,157 to 2,766,106
_2440
Pf1N1B4_2441 - Electron transfer flavoprotein, alpha subunit, at 2,766,106 to 2,767,326
_2441
Pf1N1B4_2442 - Electron transfer flavoprotein, beta subunit, at 2,767,342 to 2,768,112
_2442
Pf1N1B4_2443 - acetyltransferase, GNAT family, at 2,768,301 to 2,768,867
_2443
Position (kb)
2766
2767
2768 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2765.552 kb on + strand, within Pf1N1B4_2440 at 2765.552 kb on + strand, within Pf1N1B4_2440 at 2765.553 kb on - strand, within Pf1N1B4_2440 at 2765.613 kb on - strand, within Pf1N1B4_2440 at 2765.634 kb on - strand, within Pf1N1B4_2440 at 2765.634 kb on - strand, within Pf1N1B4_2440 at 2765.657 kb on + strand, within Pf1N1B4_2440 at 2765.657 kb on + strand, within Pf1N1B4_2440 at 2765.657 kb on + strand, within Pf1N1B4_2440 at 2765.657 kb on + strand, within Pf1N1B4_2440 at 2765.658 kb on - strand, within Pf1N1B4_2440 at 2765.658 kb on - strand, within Pf1N1B4_2440 at 2765.658 kb on - strand, within Pf1N1B4_2440 at 2765.658 kb on - strand, within Pf1N1B4_2440 at 2765.658 kb on - strand, within Pf1N1B4_2440 at 2765.658 kb on - strand, within Pf1N1B4_2440 at 2765.723 kb on + strand, within Pf1N1B4_2440 at 2765.723 kb on + strand, within Pf1N1B4_2440 at 2765.723 kb on + strand, within Pf1N1B4_2440 at 2765.723 kb on + strand, within Pf1N1B4_2440 at 2765.739 kb on - strand, within Pf1N1B4_2440 at 2765.739 kb on - strand, within Pf1N1B4_2440 at 2765.762 kb on + strand, within Pf1N1B4_2440 at 2765.781 kb on + strand, within Pf1N1B4_2440 at 2765.782 kb on - strand, within Pf1N1B4_2440 at 2766.253 kb on - strand, within Pf1N1B4_2441 at 2766.373 kb on + strand, within Pf1N1B4_2441 at 2766.373 kb on + strand, within Pf1N1B4_2441 at 2766.373 kb on + strand, within Pf1N1B4_2441 at 2766.374 kb on - strand, within Pf1N1B4_2441 at 2766.374 kb on - strand, within Pf1N1B4_2441 at 2766.374 kb on - strand, within Pf1N1B4_2441 at 2766.483 kb on - strand, within Pf1N1B4_2441 at 2766.483 kb on - strand at 2766.568 kb on + strand, within Pf1N1B4_2441 at 2767.120 kb on + strand, within Pf1N1B4_2441 at 2767.121 kb on - strand, within Pf1N1B4_2441 at 2767.286 kb on + strand at 2767.286 kb on + strand at 2767.342 kb on - strand at 2767.510 kb on + strand, within Pf1N1B4_2442 at 2767.511 kb on - strand, within Pf1N1B4_2442 at 2767.588 kb on + strand, within Pf1N1B4_2442 at 2767.588 kb on + strand, within Pf1N1B4_2442 at 2767.589 kb on - strand, within Pf1N1B4_2442 at 2767.589 kb on - strand, within Pf1N1B4_2442 at 2767.589 kb on - strand, within Pf1N1B4_2442 at 2767.790 kb on + strand at 2767.790 kb on + strand, within Pf1N1B4_2442 at 2767.790 kb on + strand, within Pf1N1B4_2442 at 2767.791 kb on - strand, within Pf1N1B4_2442 at 2767.872 kb on - strand, within Pf1N1B4_2442 at 2768.290 kb on - strand at 2768.299 kb on + strand at 2768.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 2,765,552 + Pf1N1B4_2440 0.72 +0.7 2,765,552 + Pf1N1B4_2440 0.72 -1.9 2,765,553 - Pf1N1B4_2440 0.72 +0.3 2,765,613 - Pf1N1B4_2440 0.75 +1.8 2,765,634 - Pf1N1B4_2440 0.76 +0.5 2,765,634 - Pf1N1B4_2440 0.76 -0.7 2,765,657 + Pf1N1B4_2440 0.77 -0.0 2,765,657 + Pf1N1B4_2440 0.77 +2.3 2,765,657 + Pf1N1B4_2440 0.77 -0.2 2,765,657 + Pf1N1B4_2440 0.77 +0.8 2,765,658 - Pf1N1B4_2440 0.77 -0.2 2,765,658 - Pf1N1B4_2440 0.77 -0.5 2,765,658 - Pf1N1B4_2440 0.77 -1.5 2,765,658 - Pf1N1B4_2440 0.77 -0.2 2,765,658 - Pf1N1B4_2440 0.77 +0.3 2,765,658 - Pf1N1B4_2440 0.77 +1.3 2,765,723 + Pf1N1B4_2440 0.80 -0.3 2,765,723 + Pf1N1B4_2440 0.80 +0.0 2,765,723 + Pf1N1B4_2440 0.80 -1.7 2,765,723 + Pf1N1B4_2440 0.80 -1.0 2,765,739 - Pf1N1B4_2440 0.81 -0.2 2,765,739 - Pf1N1B4_2440 0.81 -0.7 2,765,762 + Pf1N1B4_2440 0.82 -0.7 2,765,781 + Pf1N1B4_2440 0.83 -1.3 2,765,782 - Pf1N1B4_2440 0.83 -1.1 2,766,253 - Pf1N1B4_2441 0.12 +1.1 2,766,373 + Pf1N1B4_2441 0.22 -0.7 2,766,373 + Pf1N1B4_2441 0.22 -1.1 2,766,373 + Pf1N1B4_2441 0.22 -0.5 2,766,374 - Pf1N1B4_2441 0.22 -1.5 2,766,374 - Pf1N1B4_2441 0.22 -0.3 2,766,374 - Pf1N1B4_2441 0.22 -0.4 2,766,483 - Pf1N1B4_2441 0.31 -1.1 2,766,483 - +0.3 2,766,568 + Pf1N1B4_2441 0.38 -0.9 2,767,120 + Pf1N1B4_2441 0.83 -0.0 2,767,121 - Pf1N1B4_2441 0.83 -1.0 2,767,286 + -0.2 2,767,286 + +0.5 2,767,342 - +2.3 2,767,510 + Pf1N1B4_2442 0.22 -1.1 2,767,511 - Pf1N1B4_2442 0.22 +1.6 2,767,588 + Pf1N1B4_2442 0.32 -0.0 2,767,588 + Pf1N1B4_2442 0.32 +1.0 2,767,589 - Pf1N1B4_2442 0.32 +0.3 2,767,589 - Pf1N1B4_2442 0.32 +1.3 2,767,589 - Pf1N1B4_2442 0.32 -0.7 2,767,790 + +1.3 2,767,790 + Pf1N1B4_2442 0.58 +2.6 2,767,790 + Pf1N1B4_2442 0.58 -0.2 2,767,791 - Pf1N1B4_2442 0.58 +1.8 2,767,872 - Pf1N1B4_2442 0.69 -0.6 2,768,290 - -0.7 2,768,299 + -0.1 2,768,300 - +0.5
Or see this region's nucleotide sequence