Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_200 and Pf1N1B4_201 overlap by 4 nucleotides Pf1N1B4_201 and Pf1N1B4_202 overlap by 1 nucleotides Pf1N1B4_202 and Pf1N1B4_203 are separated by 0 nucleotides
Pf1N1B4_200 - FIG014801: Cation ABC transporter, periplasmic cation-binding protein, at 247,240 to 248,169
_200
Pf1N1B4_201 - ABC transporter in pyoverdin gene cluster, ATP-binding component, at 248,166 to 249,017
_201
Pf1N1B4_202 - ABC transporter in pyoverdin gene cluster, permease component, at 249,017 to 249,916
_202
Pf1N1B4_203 - ABC transporter in pyoverdin gene cluster, periplasmic component, at 249,917 to 250,888
_203
Position (kb) 249
250 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 248.020 kb on + strand, within Pf1N1B4_200 at 248.020 kb on + strand, within Pf1N1B4_200 at 248.021 kb on - strand, within Pf1N1B4_200 at 248.524 kb on - strand, within Pf1N1B4_201 at 248.558 kb on - strand, within Pf1N1B4_201 at 248.624 kb on + strand, within Pf1N1B4_201 at 248.625 kb on - strand, within Pf1N1B4_201 at 248.632 kb on - strand, within Pf1N1B4_201 at 249.023 kb on + strand at 249.024 kb on - strand at 249.251 kb on + strand at 249.251 kb on + strand, within Pf1N1B4_202 at 249.252 kb on - strand at 249.264 kb on - strand at 249.311 kb on + strand, within Pf1N1B4_202 at 249.311 kb on + strand at 249.311 kb on + strand at 249.311 kb on + strand, within Pf1N1B4_202 at 249.311 kb on + strand, within Pf1N1B4_202 at 249.312 kb on - strand, within Pf1N1B4_202 at 249.315 kb on + strand, within Pf1N1B4_202 at 249.315 kb on + strand, within Pf1N1B4_202 at 249.316 kb on - strand, within Pf1N1B4_202 at 249.316 kb on - strand, within Pf1N1B4_202 at 249.326 kb on - strand, within Pf1N1B4_202 at 249.533 kb on + strand, within Pf1N1B4_202 at 249.533 kb on + strand, within Pf1N1B4_202 at 249.534 kb on - strand, within Pf1N1B4_202 at 249.608 kb on + strand, within Pf1N1B4_202 at 250.103 kb on + strand, within Pf1N1B4_203 at 250.103 kb on + strand, within Pf1N1B4_203 at 250.104 kb on - strand, within Pf1N1B4_203 at 250.194 kb on - strand, within Pf1N1B4_203 at 250.226 kb on - strand, within Pf1N1B4_203 at 250.313 kb on - strand, within Pf1N1B4_203 at 250.500 kb on + strand, within Pf1N1B4_203 at 250.501 kb on - strand, within Pf1N1B4_203 at 250.737 kb on + strand, within Pf1N1B4_203 at 250.737 kb on + strand, within Pf1N1B4_203 at 250.737 kb on + strand, within Pf1N1B4_203 at 250.738 kb on - strand, within Pf1N1B4_203
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 248,020 + Pf1N1B4_200 0.84 +0.8 248,020 + Pf1N1B4_200 0.84 +0.9 248,021 - Pf1N1B4_200 0.84 -0.2 248,524 - Pf1N1B4_201 0.42 -1.2 248,558 - Pf1N1B4_201 0.46 +2.8 248,624 + Pf1N1B4_201 0.54 -1.2 248,625 - Pf1N1B4_201 0.54 +0.4 248,632 - Pf1N1B4_201 0.55 +0.2 249,023 + -0.2 249,024 - +0.8 249,251 + -1.8 249,251 + Pf1N1B4_202 0.26 -1.7 249,252 - -1.2 249,264 - +1.8 249,311 + Pf1N1B4_202 0.33 -0.2 249,311 + -0.2 249,311 + +0.8 249,311 + Pf1N1B4_202 0.33 -0.5 249,311 + Pf1N1B4_202 0.33 -1.2 249,312 - Pf1N1B4_202 0.33 +2.1 249,315 + Pf1N1B4_202 0.33 +1.0 249,315 + Pf1N1B4_202 0.33 -0.8 249,316 - Pf1N1B4_202 0.33 -2.5 249,316 - Pf1N1B4_202 0.33 +0.8 249,326 - Pf1N1B4_202 0.34 -2.2 249,533 + Pf1N1B4_202 0.57 -1.8 249,533 + Pf1N1B4_202 0.57 +1.1 249,534 - Pf1N1B4_202 0.57 -2.5 249,608 + Pf1N1B4_202 0.66 -0.2 250,103 + Pf1N1B4_203 0.19 -0.0 250,103 + Pf1N1B4_203 0.19 -0.5 250,104 - Pf1N1B4_203 0.19 -0.2 250,194 - Pf1N1B4_203 0.28 +0.5 250,226 - Pf1N1B4_203 0.32 +0.5 250,313 - Pf1N1B4_203 0.41 -1.8 250,500 + Pf1N1B4_203 0.60 -0.6 250,501 - Pf1N1B4_203 0.60 +0.5 250,737 + Pf1N1B4_203 0.84 -1.2 250,737 + Pf1N1B4_203 0.84 -0.8 250,737 + Pf1N1B4_203 0.84 -1.2 250,738 - Pf1N1B4_203 0.84 -0.2
Or see this region's nucleotide sequence