Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1442 and Pf1N1B4_1443 are separated by 87 nucleotides Pf1N1B4_1443 and Pf1N1B4_1444 overlap by 10 nucleotides Pf1N1B4_1444 and Pf1N1B4_1445 are separated by 228 nucleotides
Pf1N1B4_1442 - 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), at 1,654,182 to 1,655,021
_1442
Pf1N1B4_1443 - FIG074102: hypothetical protein, at 1,655,109 to 1,655,561
_1443
Pf1N1B4_1444 - ADP-ribose pyrophosphatase (EC 3.6.1.13), at 1,655,552 to 1,656,169
_1444
Pf1N1B4_1445 - lipoprotein, putative, at 1,656,398 to 1,657,144
_1445
Position (kb)
1655
1656 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1654.133 kb on + strand at 1654.133 kb on + strand at 1654.133 kb on + strand at 1654.133 kb on + strand at 1654.133 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.139 kb on - strand at 1654.139 kb on - strand at 1654.169 kb on + strand at 1654.201 kb on + strand at 1655.030 kb on + strand at 1655.030 kb on + strand at 1655.031 kb on - strand at 1655.031 kb on - strand at 1655.043 kb on + strand at 1655.043 kb on + strand at 1655.044 kb on - strand at 1655.044 kb on - strand at 1655.044 kb on - strand at 1655.097 kb on + strand at 1655.097 kb on + strand at 1655.097 kb on + strand at 1655.097 kb on + strand at 1655.098 kb on - strand at 1655.098 kb on - strand at 1655.098 kb on - strand at 1655.125 kb on + strand at 1655.125 kb on + strand at 1655.125 kb on + strand at 1655.126 kb on - strand at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.227 kb on + strand, within Pf1N1B4_1443 at 1655.228 kb on - strand, within Pf1N1B4_1443 at 1655.228 kb on - strand, within Pf1N1B4_1443 at 1655.290 kb on + strand, within Pf1N1B4_1443 at 1655.291 kb on - strand, within Pf1N1B4_1443 at 1655.365 kb on + strand, within Pf1N1B4_1443 at 1655.365 kb on + strand, within Pf1N1B4_1443 at 1655.365 kb on + strand, within Pf1N1B4_1443 at 1655.489 kb on - strand, within Pf1N1B4_1443 at 1655.489 kb on - strand, within Pf1N1B4_1443 at 1655.553 kb on + strand at 1655.629 kb on + strand, within Pf1N1B4_1444 at 1655.629 kb on + strand, within Pf1N1B4_1444 at 1655.629 kb on + strand, within Pf1N1B4_1444 at 1655.630 kb on - strand, within Pf1N1B4_1444 at 1655.630 kb on - strand, within Pf1N1B4_1444 at 1655.630 kb on - strand, within Pf1N1B4_1444 at 1655.630 kb on - strand, within Pf1N1B4_1444 at 1655.630 kb on - strand, within Pf1N1B4_1444 at 1655.687 kb on - strand, within Pf1N1B4_1444 at 1655.687 kb on - strand, within Pf1N1B4_1444 at 1655.751 kb on + strand, within Pf1N1B4_1444 at 1655.752 kb on - strand, within Pf1N1B4_1444 at 1655.752 kb on - strand at 1655.752 kb on - strand, within Pf1N1B4_1444 at 1655.794 kb on - strand, within Pf1N1B4_1444 at 1655.822 kb on + strand, within Pf1N1B4_1444 at 1655.871 kb on - strand, within Pf1N1B4_1444 at 1655.988 kb on + strand, within Pf1N1B4_1444 at 1656.096 kb on + strand, within Pf1N1B4_1444 at 1656.096 kb on + strand, within Pf1N1B4_1444 at 1656.096 kb on + strand, within Pf1N1B4_1444 at 1656.096 kb on + strand, within Pf1N1B4_1444 at 1656.097 kb on - strand, within Pf1N1B4_1444 at 1656.097 kb on - strand, within Pf1N1B4_1444 at 1656.097 kb on - strand, within Pf1N1B4_1444 at 1656.108 kb on + strand at 1656.108 kb on + strand at 1656.215 kb on - strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.313 kb on + strand at 1656.314 kb on - strand at 1656.314 kb on - strand at 1656.317 kb on + strand at 1656.317 kb on + strand at 1656.318 kb on - strand at 1656.318 kb on - strand at 1656.318 kb on - strand at 1656.318 kb on - strand at 1656.318 kb on - strand at 1656.322 kb on + strand at 1656.322 kb on + strand at 1656.322 kb on + strand at 1656.387 kb on - strand at 1656.477 kb on - strand, within Pf1N1B4_1445 at 1656.477 kb on - strand, within Pf1N1B4_1445 at 1656.496 kb on + strand, within Pf1N1B4_1445 at 1656.496 kb on + strand, within Pf1N1B4_1445
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 1,654,133 + +0.5 1,654,133 + +2.7 1,654,133 + -0.8 1,654,133 + +1.2 1,654,133 + -0.0 1,654,138 + +0.1 1,654,138 + -1.3 1,654,138 + +1.0 1,654,138 + -0.3 1,654,138 + -1.2 1,654,138 + -2.6 1,654,139 - -1.3 1,654,139 - +0.2 1,654,169 + -0.8 1,654,201 + -2.6 1,655,030 + +1.2 1,655,030 + +2.8 1,655,031 - -0.8 1,655,031 - +1.8 1,655,043 + -1.1 1,655,043 + -0.1 1,655,044 - -1.3 1,655,044 - +1.5 1,655,044 - -0.1 1,655,097 + -2.6 1,655,097 + +0.0 1,655,097 + +0.4 1,655,097 + -0.2 1,655,098 - +0.2 1,655,098 - -0.6 1,655,098 - -0.9 1,655,125 + -0.8 1,655,125 + -0.3 1,655,125 + +0.2 1,655,126 - +0.2 1,655,227 + Pf1N1B4_1443 0.26 +0.2 1,655,227 + Pf1N1B4_1443 0.26 -0.8 1,655,227 + Pf1N1B4_1443 0.26 -2.0 1,655,227 + Pf1N1B4_1443 0.26 -0.8 1,655,227 + Pf1N1B4_1443 0.26 -1.1 1,655,227 + Pf1N1B4_1443 0.26 -0.8 1,655,227 + Pf1N1B4_1443 0.26 -1.8 1,655,227 + Pf1N1B4_1443 0.26 +0.1 1,655,227 + Pf1N1B4_1443 0.26 +0.2 1,655,228 - Pf1N1B4_1443 0.26 +0.7 1,655,228 - Pf1N1B4_1443 0.26 -1.6 1,655,290 + Pf1N1B4_1443 0.40 +2.8 1,655,291 - Pf1N1B4_1443 0.40 -0.2 1,655,365 + Pf1N1B4_1443 0.57 -1.5 1,655,365 + Pf1N1B4_1443 0.57 -0.8 1,655,365 + Pf1N1B4_1443 0.57 -1.3 1,655,489 - Pf1N1B4_1443 0.84 -0.1 1,655,489 - Pf1N1B4_1443 0.84 +0.8 1,655,553 + +0.5 1,655,629 + Pf1N1B4_1444 0.12 +0.2 1,655,629 + Pf1N1B4_1444 0.12 -1.0 1,655,629 + Pf1N1B4_1444 0.12 -0.1 1,655,630 - Pf1N1B4_1444 0.13 -1.5 1,655,630 - Pf1N1B4_1444 0.13 -0.3 1,655,630 - Pf1N1B4_1444 0.13 +3.4 1,655,630 - Pf1N1B4_1444 0.13 +0.8 1,655,630 - Pf1N1B4_1444 0.13 -2.1 1,655,687 - Pf1N1B4_1444 0.22 +0.2 1,655,687 - Pf1N1B4_1444 0.22 +0.2 1,655,751 + Pf1N1B4_1444 0.32 +0.2 1,655,752 - Pf1N1B4_1444 0.32 +0.2 1,655,752 - +1.2 1,655,752 - Pf1N1B4_1444 0.32 +0.2 1,655,794 - Pf1N1B4_1444 0.39 +0.4 1,655,822 + Pf1N1B4_1444 0.44 -1.8 1,655,871 - Pf1N1B4_1444 0.52 -0.8 1,655,988 + Pf1N1B4_1444 0.71 +0.2 1,656,096 + Pf1N1B4_1444 0.88 +0.2 1,656,096 + Pf1N1B4_1444 0.88 -0.8 1,656,096 + Pf1N1B4_1444 0.88 -1.8 1,656,096 + Pf1N1B4_1444 0.88 -0.3 1,656,097 - Pf1N1B4_1444 0.88 -0.8 1,656,097 - Pf1N1B4_1444 0.88 -0.1 1,656,097 - Pf1N1B4_1444 0.88 -0.2 1,656,108 + -0.3 1,656,108 + -0.3 1,656,215 - +1.0 1,656,313 + -0.1 1,656,313 + +0.2 1,656,313 + +0.2 1,656,313 + +0.0 1,656,313 + -1.8 1,656,313 + +0.2 1,656,313 + -0.8 1,656,314 - -1.1 1,656,314 - -1.3 1,656,317 + -0.6 1,656,317 + -0.8 1,656,318 - -0.0 1,656,318 - +1.5 1,656,318 - +0.6 1,656,318 - +0.7 1,656,318 - +0.2 1,656,322 + -0.6 1,656,322 + +0.2 1,656,322 + +3.2 1,656,387 - -1.3 1,656,477 - Pf1N1B4_1445 0.11 +1.0 1,656,477 - Pf1N1B4_1445 0.11 -0.3 1,656,496 + Pf1N1B4_1445 0.13 +0.1 1,656,496 + Pf1N1B4_1445 0.13 -0.8
Or see this region's nucleotide sequence